杉木极性生长素运输增强和油菜素内酯活性降低驱动节间伸长
{"_":"Enhanced Polar Auxin Transport and Reduced Brassinosteroid Activity Drive Internode Elongation in Chinese Fir ().","i":["Cunninghamia lanceolata"]}
摘要(中文译文)
无节材生产关键取决于节间特征，然而调控节间伸长的机制仍知之甚少，这阻碍了培育长节间品种的育种工作。本研究选取了两个节间性状差异明显的无性系（C1无性系节间长度比C11无性系长25.03%）作为材料。采用酶联免疫吸附测定（ELISA）和RNA测序技术研究节间生长中内源激素的动态变化及转录调控。结果表明，顶芽中吲哚-3-乙酸（IAA）节律的差异是C1节间伸长生长的关键因素；C1顶芽中较高水平的IAA和细胞分裂素可能支持节间的持续生长；上部韧皮部中上调的IAA相关基因（和），参与极性运输和信号响应，表明其建立顶端优势的能力更强。激素运输可能受极长链脂肪酸（VLCFAs）调控。与油菜素甾醇活性降低相一致，参与VLCFA生物合成和运输的基因在C1中普遍较低，暗示C11中过量的VLCFA积累可能对IAA运输和节间生长产生负面影响。本研究为激素生物合成和运输影响节间生长机制提供了初步见解，为无节材培育中的遗传改良、种质选择和外源激素应用提供了依据。
Abstract
Knot-free timber production in depends critically on internodal characteristics, yet the mechanisms governing internode elongation remain poorly understood, hindering breeding efforts for longer-internode varieties. In this study, we selected two clones with distinct internodal traits (the C1 clone exhibited a 25.03% longer internodal length than the C11 clone) as materials. Enzyme-linked immunosorbent assay (ELISA) and RNA sequencing were used to investigate dynamics in endogenous hormones and transcriptional regulation in internodal growth. Results showed that the difference in indole-3-acetic acid (IAA) rhythms in apical buds is a key factor of C1's longer internodal growth; higher levels of IAA and cytokinins in the apical buds of C1 may support sustained internodal growth; upregulated IAA-related genes in upper phloem ( and ), which are involved in polar transport and signal response, indicates a stronger capacity to establish apical dominance. Hormone transport may be regulated by very long-chain fatty acids (VLCFAs). Consistent with reduced brassinosteroid activity, genes involved in VLCFA biosynthesis and transport were generally lower in C1, implying excessive VLCFA accumulation in C11 may be negative to IAA transporting and internode growth. This study offers a preliminary insight into internodal growth mechanisms influenced by hormone biosynthesis and transport in ., providing a basis for genetic improvement, germplasm selection, and exogenous hormone applications in knot-free timber cultivation.
如何引用
AMA
Chao Wu, Fang-Fang Wang, Fang-Fang Ma, Ling-Peng Ye, Shi-Yan Mu, Ya-Ting Yang, et al. {"_":"Enhanced Polar Auxin Transport and Reduced Brassinosteroid Activity Drive Internode Elongation in Chinese Fir ().","i":["Cunninghamia lanceolata"]}. Plants (Basel, Switzerland). 2026; doi:10.3390/plants15091411.
APA
Chao Wu, Fang-Fang Wang, Fang-Fang Ma, Ling-Peng Ye, Shi-Yan Mu, Ya-Ting Yang, et al (2026). {"_":"Enhanced Polar Auxin Transport and Reduced Brassinosteroid Activity Drive Internode Elongation in Chinese Fir ().","i":["Cunninghamia lanceolata"]}. Plants (Basel, Switzerland). https://doi.org/10.3390/plants15091411
GB/T 7714
Chao Wu, Fang-Fang Wang, Fang-Fang Ma, Ling-Peng Ye, Shi-Yan Mu, Ya-Ting Yang, et al. {"_":"Enhanced Polar Auxin Transport and Reduced Brassinosteroid Activity Drive Internode Elongation in Chinese Fir ().","i":["Cunninghamia lanceolata"]}[J]. Plants (Basel, Switzerland), 2026 doi:10.3390/plants15091411.
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