燕麦14-3-3基因家族的全基因组鉴定和特征分析
{"_":"Genome-Wide Identification and Characterization of the Gene Family in .","i":["14-3-3","Avena sativa"]}
摘要(中文译文)
14-3-3蛋白是高度保守的调控蛋白，整合了调控植物生长、发育和胁迫响应的信号通路。然而，燕麦（Avena sativa）中的该基因家族尚未被系统研究。本研究对燕麦该基因家族进行了全面分析，包括其理化性质、基因结构、系统发育、保守基序、启动子顺式作用元件以及选择压力。共鉴定出19个该基因家族成员，并将其分为ε组和非ε组。该基因家族主要通过片段重复进行扩张，并在进化过程中经历了强烈的纯化选择。qRT-PCR分析显示，有6个该基因家族成员在干旱胁迫下的一个或多个时间点显著上调。值得注意的是，其中一个成员表现出持续且显著的上调。亚细胞定位分析表明，AsGF14k定位于细胞核和细胞质。此外，酵母双杂交实验表明AsGF14k不形成同源二聚体。本研究结果系统地表征了该基因家族及其干旱响应表达模式，为该基因家族在干旱胁迫下的功能验证奠定了初步基础。
Abstract
14-3-3 proteins are highly conserved regulatory proteins that integrate signaling pathways governing plant growth, development, and stress responses. However, the gene family in oat () has not been systematically investigated. Here, we performed a comprehensive analysis of oat genes, including their physicochemical properties, gene structures, phylogeny, conserved motifs, promoter -elements, and selective pressures. A total of 19 genes were identified and classified into the ε and non-ε groups. The gene family expanded primarily through segmental duplications and has been under strong purifying selection during evolution. qRT-PCR analysis revealed that six genes were significantly upregulated at one or more time points under drought stress. Notably, exhibited sustained and significant upregulation. Subcellular localization analysis showed that AsGF14k localized to both the nucleus and the cytoplasm. Furthermore, Y2H assays indicated that AsGF14k does not form homodimers. Our results provide a systematic characterization of the gene family and their drought-responsive expression patterns, establishing a preliminary basis for the functional validation of genes under drought stress.
如何引用
AMA
Shirui Xu, Mingchuan Ma, Zhang Liu, Lijun Zhang, Longlong Liu. {"_":"Genome-Wide Identification and Characterization of the Gene Family in .","i":["14-3-3","Avena sativa"]}. Plants (Basel, Switzerland). 2026; doi:10.3390/plants15091280.
APA
Shirui Xu, Mingchuan Ma, Zhang Liu, Lijun Zhang, Longlong Liu (2026). {"_":"Genome-Wide Identification and Characterization of the Gene Family in .","i":["14-3-3","Avena sativa"]}. Plants (Basel, Switzerland). https://doi.org/10.3390/plants15091280
GB/T 7714
Shirui Xu, Mingchuan Ma, Zhang Liu, Lijun Zhang, Longlong Liu. {"_":"Genome-Wide Identification and Characterization of the Gene Family in .","i":["14-3-3","Avena sativa"]}[J]. Plants (Basel, Switzerland), 2026 doi:10.3390/plants15091280.
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