绝经后骨质疏松症肠道微生物群的全面分析
Comprehensive profiling of gut microbiota in postmenopausal osteoporosis.
摘要(中文译文)
骨质疏松症是一种多因素骨骼疾病，主要影响绝经后女性，以骨量减少和骨折风险增加为特征。越来越多的证据表明，肠道微生物群通过免疫、内分泌和代谢途径在骨代谢中发挥关键作用，构成了所谓“肠-骨轴”的基础。本研究旨在比较绝经后骨质疏松症女性与健康对照者的肠道微生物群组成特征。根据双能X线吸收法T值，将40名年龄55~65岁的绝经后女性分为骨质疏松组（n = 20）和健康对照组（n = 20）。采集粪便样本，并采用Oxford Nanopore Technologies进行全长16S rRNA基因测序以评估肠道微生物群组成。除骨质疏松组骨密度（BMD）如预期降低外，两组间临床或人口学特征均无显著差异。α多样性指数未显示统计学显著差异，但在骨质疏松参与者中观察到微生物丰富度降低的趋势。β多样性分析通过加权UniFrac分析揭示了适度但显著的系统发育差异（p < 0.05）。产短链脂肪酸的菌种，包括 、 和 ，在骨质疏松组中显著减少。 丰度与股骨颈T值呈正相关（r = 0.41，p = 0.018），而 与腰椎BMD呈负相关（r = -0.43，p = 0.015）。绝经后骨质疏松症与一种独特的肠道微生物群谱相关，其特征为抗炎和雌激素调节类群减少，同时促炎菌种增加。这些改变可能通过破坏钙吸收、系统性炎症和激素失调导致骨代谢受损。这些发现进一步支持了肠-骨轴，并突出了肠道微生物群作为骨质疏松症生物标志物和治疗靶点的潜力。
Abstract
Osteoporosis is a multifactorial skeletal disease that predominantly affects postmenopausal women and is characterized by reduced bone mass and an increased risk of fractures. Growing evidence suggests that the gut microbiota plays a pivotal role in bone metabolism through immunological, endocrine, and metabolic pathways, forming the basis of the so-called gut-bone axis. This study aimed to characterize gut microbiota composition in postmenopausal women with osteoporosis compared with healthy controls. Forty postmenopausal women aged 55-65 years were classified into osteoporosis (n = 20) and healthy control (n = 20) groups based on dual-energy X-ray absorptiometry T-scores. Fecal samples were collected, and gut microbiota composition was evaluated with full-length 16S rRNA gene sequencing using Oxford Nanopore Technologies. No significant differences were observed in clinical or demographic characteristics between groups, except for the expected lower bone mineral density (BMD) in the osteoporosis group. Alpha-diversity indices showed no statistically significant differences, although a trend toward reduced microbial richness was observed in the osteoporotic participants. Beta-diversity analysis revealed a modest but significant phylogenetic distinction via weighted UniFrac analysis (p < 0.05). Short-chain fatty acid-producing species, including , , and , were significantly reduced in the osteoporosis group. abundance was positively correlated with the femoral neck T-score (r = 0.41, p = 0.018), whereas showed a negative correlation with lumbar spine BMD (r = -0.43, p = 0.015). Postmenopausal osteoporosis is associated with a distinct gut microbiota profile marked by reduced antiinflammatory and estrogen-modulating taxa alongside increased proinflammatory species. These alterations may contribute to impaired bone metabolism through disrupted calcium absorption, systemic inflammation, and hormonal dysregulation. These findings further support the gut-bone axis and highlight the potential of gut microbiota as a biomarker and therapeutic target in osteoporosis.
如何引用
AMA
Gülsemin Ertürk Çelik, Sedat Yildiz, Münevver Aksoy Bektaş, Yaprak Engin Üstün. Comprehensive profiling of gut microbiota in postmenopausal osteoporosis.. Turkish journal of medical sciences. 2026; doi:10.55730/1300-0144.6187.
APA
Gülsemin Ertürk Çelik, Sedat Yildiz, Münevver Aksoy Bektaş, Yaprak Engin Üstün (2026). Comprehensive profiling of gut microbiota in postmenopausal osteoporosis.. Turkish journal of medical sciences. https://doi.org/10.55730/1300-0144.6187
GB/T 7714
Gülsemin Ertürk Çelik, Sedat Yildiz, Münevver Aksoy Bektaş, Yaprak Engin Üstün. Comprehensive profiling of gut microbiota in postmenopausal osteoporosis.[J]. Turkish journal of medical sciences, 2026 doi:10.55730/1300-0144.6187.
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