人骨巨噬细胞的分离
Isolation of Human Osteal Macrophages.
摘要(中文译文)
骨巨噬细胞（骨巨噬细胞）是驻留于骨组织中的巨噬细胞，支持成骨细胞分化和骨形成。尽管其在骨稳态中具有重要作用，但其在人类骨代谢和骨质疏松症中的功能仍知之甚少，这在很大程度上是由于缺乏标准化的分离方案。在此，我们提出了一种从关节置换术中获取的股骨头标本中分离原代人骨巨噬细胞的方案。在去除骨髓以尽量减少骨髓来源巨噬细胞的污染后，对骨碎片进行酶消化，并使用基于CD14的磁激活细胞分选（MACS）或基于CD14/CD45/ALP的荧光激活细胞分选（FACS）分离骨巨噬细胞。免疫荧光证实了其巨噬细胞身份，并揭示了与M1样和M2样活化状态相关标志物的表达。分离的细胞呈现异质性形态，并可在培养中维持。该方案能够可重复地分离人骨巨噬细胞，并为研究骨疾病和骨质疏松症中骨免疫相互作用的转化研究提供了基础。
Abstract
Osteal macrophages ("osteomacs") are resident bone macrophages that support osteoblast differentiation and bone formation. Despite their importance in bone homeostasis, their function in human bone metabolism and osteoporosis remains poorly understood, largely due to the lack of a standardized isolation protocol. Here, we present a protocol for isolating primary human osteomacs from femoral head specimens obtained during arthroplasty. After the removal of bone marrow to minimize contamination with marrow-derived macrophages, bone fragments were enzymatically digested and osteomacs were isolated using CD14-based MACS or CD14/CD45/ALP-based FACS. Immunofluorescence confirmed macrophage identity and revealed expression of markers associated with both M1-like and M2-like activation states. Isolated cells displayed heterogeneous morphology and could be maintained in culture. This protocol enables reproducible isolation of human osteomacs and provides a foundation for translational studies investigating osteoimmune interactions in bone disease and osteoporosis.
如何引用
AMA
Juliana Franziska Bousch, Stefanie Lichtenberg, Matthis Schnitker, Jenny Schlösser, Christoph Viktor Suschek, Uwe Maus, et al. Isolation of Human Osteal Macrophages.. Life (Basel, Switzerland). 2026; doi:10.3390/life16030376.
APA
Juliana Franziska Bousch, Stefanie Lichtenberg, Matthis Schnitker, Jenny Schlösser, Christoph Viktor Suschek, Uwe Maus, et al (2026). Isolation of Human Osteal Macrophages.. Life (Basel, Switzerland). https://doi.org/10.3390/life16030376
GB/T 7714
Juliana Franziska Bousch, Stefanie Lichtenberg, Matthis Schnitker, Jenny Schlösser, Christoph Viktor Suschek, Uwe Maus, et al. Isolation of Human Osteal Macrophages.[J]. Life (Basel, Switzerland), 2026 doi:10.3390/life16030376.
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