基因型柔性植物遗传转化：进展与展望

摘要(中文译文)

优良作物品种的遗传转化仍受限于基因型特异性的顽拗性和对组织培养的依赖。本综述对当前的转化平台及克服这些瓶颈的新兴策略进行了全面分析。我们考察了传统的农杆菌介导法和基因枪法，继而批判性地评估了免组织培养方法，包括花器官递送、切浸芽、注射以及病毒载体介导的转化系统。一个主要关注点是发育调节因子、嵌合体、转录因子等的应用，这些因子可提高不同基因型的再生效率。我们详细阐述了其分子机制，从染色质重塑和生长素梯度建立到伤口响应性细胞重编程。重要的是，我们探讨了发育调节因子错表达的多效性发育代价，并综述了精准控制技术，包括启动子优化和自动切除系统，这些技术能够在再生过程中实现发育调节因子的瞬时活性，同时确保植物正常发育。我们提出了一个整合这些进展的路线图，以实现适用于分子设计育种、基因型灵活的高通量转化。

Abstract

Genetic transformation of elite crop varieties remains limited by genotype-specific recalcitrance and dependence on tissue culture. This review provides a comprehensive analysis of current transformation platforms and emerging strategies to overcome these bottlenecks. We examine traditional -mediated and biolistic methods, then critically assess tissue culture-free approaches including floral-based delivery, cut-dip-budding, injection, and viral vector-mediated transformation systems. A major focus is the deployment of developmental regulators-, chimeras, transcription factors, , , and -that enhance regeneration efficiency across genotypes. We detail their molecular mechanisms, from chromatin remodeling and auxin gradient establishment to wound-responsive cell reprogramming. Importantly, we address the pleiotropic developmental costs of DR misexpression and review precision control technologies, including promoter optimization and auto-excision systems, that enable transient DRs activity during regeneration while ensuring normal plant development. We propose a roadmap for integrating these advances to achieve genotype-flexible, high-throughput transformation applicable to molecular design breeding.(1).

如何引用

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