沙棘果皮多糖：通过调节肠道微生物群的结构和改善溃疡性结肠炎
A sea buckthorn pericarp polysaccharide: Structure and amelioration of ulcerative colitis by modulating gut microbiota.
摘要(中文译文)
溃疡性结肠炎（UC）以结肠黏膜损伤、上皮屏障破坏和肠道菌群失调为特征，但目前仍缺乏有效的治疗干预手段。多糖正逐渐成为干预UC的有前景的天然候选物。沙棘作为一种传统药用植物，已被记载具有肠道保护作用；然而，其果皮来源的生物活性成分在很大程度上仍未被探索。为实现该植物资源的增值利用，本研究从沙棘果皮中分离纯化出一种具有抗UC潜在功效的生物活性均一多糖，命名为SBPP-BI（分子量=62.0 kDa）。结构分析表明，SBPP-BI的主链由T-α-Galp-(1→6)-α-Galp-(1→6)-α-Galp-(1→[4)-α-GalpA-(1→4)-α-GalpA-OMe-(1→]→4)-α-GalpA-3-OAc-(1→[2)-α-Rhap-(1→4)-α-GalpA-(1→构成，支链包括→3)-α-Araf-(1→、→5)-α-Araf-(1→和T-α-Araf-(1→。体内评价表明，SBPP-BI可显著改善葡聚糖硫酸钠诱导的结肠炎，恢复肠道屏障功能，并调节UC模型小鼠的炎症细胞因子水平。机制方面，16S rRNA测序和气相色谱-质谱分析显示，SBPP-BI可调节肠道菌群组成并恢复短链脂肪酸的产生。此外，Western blot分析表明，SBPP-BI可抑制结肠组织中TLR4/NF-κB信号通路的激活。本研究阐明了沙棘果皮中一种具有抗UC活性的多糖的独特结构，并凸显了其作为UC管理益生元潜在应用的前景。
Abstract
Ulcerative colitis (UC) is characterized by colonic mucosal injury, epithelial barrier disruption, and gut microbiota dysbiosis, yet lacks effective therapeutic interventions. Polysaccharides are emerging as promising natural candidates for UC intervention. Sea buckthorn, a traditional medicinal plant, has documented intestinal protective effects; however, its pericarp-derived bioactive components remain largely unexplored. To valorize this botanical resource, a bioactive homogeneous polysaccharide with potential efficacy against UC, designated SBPP-BI (Mw = 62.0 kDa), was isolated and purified from sea buckthorn pericarps in this study. Structural analysis revealed that SBPP-BI features a backbone of T-α-Galp-(1→6)-α-Galp-(1 → 6)-α-Galp-(1 → [4)-α-GalpA-(1 → 4)-α-GalpA-OMe-(1→] → 4)-α-GalpA-3-OAc-(1 → [2)-α-Rhap-(1 → 4)-α-GalpA-(1→] with branched chains including →3)-α-Araf-(1→, →5)-α-Araf-(1→, and T-α-Araf-(1→. In vivo evaluation demonstrated that SBPP-BI markedly ameliorated dextran sulfate sodium-mediated colitis, restored intestinal barrier function, and modulated inflammatory cytokine levels in UC model mice. Mechanistically, 16S rRNA sequencing and gas chromatography-mass spectrometry analysis showed that SBPP-BI modulated gut microbiota composition and restored short-chain fatty acid production. Furthermore, Western blot analysis demonstrated that SBPP-BI suppressed activation of the TLR4/NF-κB signaling pathway in colon tissues. This research elucidates the distinct structure of a polysaccharide from sea buckthorn pericarp with anti-UC activity and highlights its promise as a prebiotic potential for UC management.
如何引用
AMA
Jing-Chun Sun, Shu-Yao Wang, Qiu-Na Nong, Yuan Guo, Yu-Fan Wang, Wen-Jing Zhao, et al. A sea buckthorn pericarp polysaccharide: Structure and amelioration of ulcerative colitis by modulating gut microbiota.. International journal of biological macromolecules. 2026; doi:10.1016/j.ijbiomac.2026.151310.
APA
Jing-Chun Sun, Shu-Yao Wang, Qiu-Na Nong, Yuan Guo, Yu-Fan Wang, Wen-Jing Zhao, et al (2026). A sea buckthorn pericarp polysaccharide: Structure and amelioration of ulcerative colitis by modulating gut microbiota.. International journal of biological macromolecules. https://doi.org/10.1016/j.ijbiomac.2026.151310
GB/T 7714
Jing-Chun Sun, Shu-Yao Wang, Qiu-Na Nong, Yuan Guo, Yu-Fan Wang, Wen-Jing Zhao, et al. A sea buckthorn pericarp polysaccharide: Structure and amelioration of ulcerative colitis by modulating gut microbiota.[J]. International journal of biological macromolecules, 2026 doi:10.1016/j.ijbiomac.2026.151310.
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