从中华鳖中发现了一种新的辛德比斯病毒
Discovery of a novel Sindbis virus from Chinese soft-shelled turtle, Pelodiscus sinensis.
摘要(中文译文)
中华鳖（Pelodiscus sinensis）是中国水产养殖中一种具有重要经济价值的物种。近期，中华鳖浮水病（CSSTFD）的暴发导致中华鳖幼苗几乎100%死亡。患病鳖偏好浮于水面，并在四天内死亡。在本研究中，我们对这些鳖体内的病毒进行了鉴定，该病毒与辛德毕斯病毒（SINV）具有高度相似性。我们采用序列非依赖性单引物扩增（SISPA）和cDNA末端快速扩增（RACE）技术对病毒基因组进行了测序。除polyA尾外，全基因组长度为11700个核苷酸，G+C含量为51.76%。BlastN分析显示，该分离株（命名为SV-PsXY1）与辛德毕斯病毒株YN222的核苷酸同一性为99.24%。该病毒基因组具有典型的甲病毒组织结构，推测编码两个大的开放阅读框（结构蛋白ORF和非结构蛋白ORF）。其氨基酸序列与SINV相应蛋白的成对同一性在82.86%至100%之间。据我们所知，这是首次在水生爬行动物中报道SINV，这扩展了该病毒的已知宿主范围，并凸显了研究其起源、传播途径及人兽共患潜力的必要性。
Abstract
The Chinese soft-shelled turtle (Pelodiscus sinensis) is an economically important species in Chinese aquaculture. Recently, Chinese soft-shelled turtle water-floating disease (CSSTFD) outbreak caused almost 100% death in Chinese soft shelled turtle seedlings. The diseased turtles preferred to float to the surface of water and then died within four days. In this study, we charaterized the virus,which showed high similarity with Sindbis virus(SINV), in these turtles. We sequenced the viral genome using sequence independent single primer amplification(SISPA) and rapid amplification of cDNA ends(RACE). The whole genome, excluding the polyA tail, is 11700 nts in length and G + C content was 51.76%. BlastN analysis revealed that the isolate, designated SV-PsXY1, shares 99.24% nucleotide identity with Sindbis virus strain YN222. This virus genome contained a typical alphavirus organization and putatively encoded two large open reading frames(structural ORF and nonstructural ORF). Pairwise amino acid identities ranged between 82.86% and 100% with the corresponding proteins of SINV. To our knowledge, this is the first report of SINV in an aquatic reptile, which expands the known host range of this virus and highlights the need to investigate its origin, transmission routes, and zoonotic potential.
如何引用
AMA
Zhigao Zhan, Zhongyi Wu, Jinchang Wang, Hongxiu Wang, Jian Yang, Limei Guan, et al. Discovery of a novel Sindbis virus from Chinese soft-shelled turtle, Pelodiscus sinensis.. Archives of virology. 2026; doi:10.1007/s00705-026-06525-4.
APA
Zhigao Zhan, Zhongyi Wu, Jinchang Wang, Hongxiu Wang, Jian Yang, Limei Guan, et al (2026). Discovery of a novel Sindbis virus from Chinese soft-shelled turtle, Pelodiscus sinensis.. Archives of virology. https://doi.org/10.1007/s00705-026-06525-4
GB/T 7714
Zhigao Zhan, Zhongyi Wu, Jinchang Wang, Hongxiu Wang, Jian Yang, Limei Guan, et al. Discovery of a novel Sindbis virus from Chinese soft-shelled turtle, Pelodiscus sinensis.[J]. Archives of virology, 2026 doi:10.1007/s00705-026-06525-4.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出