ARCHIVES OF VIROLOGY病毒学档案
ARCHIVES OF VIROLOGY(英文缩写 ARCH VIROL),ISSN 0304-8608,eISSN 1432-8798,中文译名:病毒学档案 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.685 | Q3 |
| 2022 | 2.700 | Q3 |
| 2023 | 2.500 | Q3 |
| 2024 | 2.500 | Q3 |
| 2025 | 2.500 | Q3 |
ARCHIVES OF VIROLOGY 最新收录文献
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2. A field deployable duplex RPA-CRISPR/Cas12a assay for rapid and sensitive detection of African swine fever virus.
PMID:日期:2026-09-24African swine fever (ASF) is a notifiable transboundary disease of domestic pigs and wild suids that causes severe haemorrhagic fever, with high mortality, and substantial socio-economic losses worldwide. In the absence of effective vaccines or therapeutics, ASF control relies primarily on early diagnosis, strict biosecurity, and stamping-out strategies. In this study, we developed a rapid, field-deployable recombinase polymerase amplification (RPA)-CRISPR/Cas12a assay for the detection of African swine fever virus using both fluorescence- and lateral flow strip (LFS)-based readouts. The assay operates isothermally at 37 °ºC and enables rapid, equipment-free visual detection suitable for on-site testing. To improve diagnostic sensitivity and robustness, a dual-target approach was employed targeting the highly conserved C-terminal region of the p72 (B646L) gene and the early-expressing p22 (KP177R) gene. In the uniplex format, fluorescence-based assays achieved limits of detection of 10.18 and 13.68 copies/reaction for p72 and KP177R, respectively, whereas the LFS format detected 1,018 and 1,368 copies/reaction. Notably, the duplex RPA-CRISPR/Cas12a assay demonstrated enhanced sensitivity with detection limits of 1.02 copies/reaction in the fluorescence format and 102 copies/reaction in the LFS format. The assay showed 100% analytical specificity against other porcine viral pathogens and strong agreement with real-time PCR. Furthermore, lyophilized reagents formulated in a two-tube format retained their analytical performance after storage at 4 °C for one week, highlighting the platform's potential for decentralized ASF surveillance and outbreak response.
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3. Shotgun metagenomic sequencing and genetic characterization of four enteric viruses in a dairy farm suffering a diarrhea outbreak of undetermined cause in adult cattle.
PMID:日期:2026-09-22Diarrhea is an acute disease of cattle that has been extensively studied in calves; however, it has been far less investigated in adult cattle, despite its significant impact on milk production and animal health. In this study, we investigated an outbreak in a dairy herd in Uruguay for which initial diagnostic testing for the main known pathogens yielded negative results. Therefore, the outbreak was further investigated in depth using shotgun metagenomic sequencing, molecular virology assays and viral isolation. Of the three fecal samples from affected cows that were submitted for diagnostic testing and were negative for the main enteric pathogens, including BCoV, two were positive for bovine torovirus (BToV) by RT-qPCR. Next-generation sequencing-based virome analysis revealed over 3,300 viral operational taxonomic units (vOTUs), predominantly bacteriophages, but also eukaryotic viruses from 11 families. The complete genome of BToV was obtained and a putative novel protoparvovirus species was characterized. In addition, two distinct enteroviruses belonging to the species Enterovirus fitauri and Enterovirus idromi were identified. Notably, Enterovirus idromi had previously been reported exclusively in dromedaries; its detection in cattle expands the known host range of this species. Furthermore, the Enterovirus idromi strain appears to represent an interspecies recombinant enterovirus. These results highlight the importance of emerging approaches such as next-generation sequencing for identifying novel pathogens in cattle diseases and may serve as a basis for evaluating their potential role in disease pathogenesis, as well as for incorporating them into routine initial diagnostic testing.
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4. Molecular detection of feline viruses in free-ranging European wildcats (Felis s. silvestris) of the Harz Mountains, Germany.
PMID:日期:2026-09-21Many infectious diseases commonly affecting domestic cats may pose potential threats to wild felid populations. Several established contagious feline viruses - including feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), feline coronavirus (FCoV), feline calicivirus (FCV), feline herpesvirus (FHV), and feline parvovirus (FPV) - are well-known drivers of disease epidemiology. In addition, recently identified agents such as feline circovirus (FeCV), feline morbillivirus (FeMV) and other novel paramyxoviruses as well as polyomaviruses may represent emerging risks to wild populations. We conducted a comprehensive molecular epidemiological survey to investigate the occurrence of these viruses in free-ranging European wildcats (Felis s. silvestris) from the Harz Mountains, a low mountain range in central Germany. A total of 42 individuals were sampled between 2010 and 2018. Molecular screening was performed using a comprehensive set of conventional PCR, reverse transcription PCR (RT-PCR), and quantitative RT-PCR (qRT-PCR) assays to detect viral nucleic acids. FeLV DNA was detected in 5/42 individuals (11.90%), and FPV DNA was detected in 2/42 wildcats (4.76%). Overall, viral nucleic acid was identified in seven animals (16.67%). No evidence of the remaining target viruses was found using the respective (q)RT-PCR and conventional PCR assays. However, negative PCR results should be interpreted with caution due to the exclusive use of long-term ethanol-preserved spleen samples, which may have reduced analytical sensitivity. The findings provide molecular evidence of FPV and FeLV, in free-ranging European wildcats in the Harz Mountains. Continued surveillance remains essential to better understand pathogen dynamics, evaluate potential spillover from domestic cats, and inform conservation strategies aimed at safeguarding wild felid populations in Central Europe.
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5. Meta-transcriptomic profiling of Ixodes ricinus and Ixodes persulcatus ticks from Finland reveals diverse RNA viromes, community structuring, and emerging viral lineages.
PMID:日期:2026-09-21Tick-borne viruses (TBVs) threaten human and animal health in northern Europe, where Ixodes ricinus and Ixodes persulcatus are expanding their range. In Finland, the genetic diversity, population structure, and tick-associated RNA virome of these species remain insufficiently characterized. Characterizing tick-borne viruses in Finland is essential to detect emerging pathogens at the northern edge of tick expansion, where climate-driven ecological change may facilitate the spread of novel viruses with potential risks to human and animal health. We applied single-tick metatranscriptomic approach to profile and compare the RNA viromes of 92 adult I. ricinus and I. persulcatus collected from two locations in Finland. We identified 42 RNA viruses spanning 12 families, with Nairoviridae, Phenuiviridae, Partitiviridae, Flaviviridae, and Rhabdoviridae being the most represented. Viral community composition differed significantly between tick species, with I. persulcatus exhibiting more constrained but bunyaviridae-dominated viromes, whereas I. ricinus showed greater inter-individual variability and enrichment of partiti-like and iflavirus taxa. Phylogenetic reconstruction of conserved viral proteins supported the presence of several divergent viral lineages and confirmed the first detection of multiple Eurasian tick-associated viruses in Finland, including Nuomin virus, Beiji nairovirus, and Onega tick phlebovirus. Several detected viruses clustered within clades that include known or suspected zoonotic agents, highlighting the need for close monitoring of tick populations, especially in zones of sympatric occurrence where hybridization and associated close contact between the tick species have been observed. These findings reveal substantial, species-structured viral diversity in Finnish Ixodes ticks.
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6. Molecular characterization of a novel polymycovirus isolated from the phytopathogenic fungus Fusarium oxysporum.
PMID:日期:2026-09-21Here, a novel double-stranded RNA (dsRNA) mycovirus, tentatively designated Fusarium oxysporum polymycovirus 1 (FoPmV1), was isolated from Fusarium oxysporum, a phytopathogenic fungus that causes tobacco root rot. The genome of FoPmV1 consists of eight dsRNA segments, referred to as dsRNA1 to dsRNA8, with lengths of 2440 bp, 2276 bp, 2042 bp, 1303 bp, 1144 bp, 1064 bp, 1004 bp, and 913 bp, respectively. Sequence analysis revealed that these dsRNA segments encode 8 open reading frames (ORFs): ORF1 (771 amino acids, aa), ORF2 (699 aa), ORF3 (622 aa), and ORF4 (304 aa) encode an RNA-dependent RNA polymerase (RdRp), scaffold protein, methyltransferase, and coat protein (PASRP), respectively. While ORF5 (304 aa), ORF6 (161 aa), ORF7 (167 aa), and ORF8 (211 aa) encode proteins with unknown functions. BLAST analysis revealed that the RdRp of FoPmV1 shares the maximal sequence identity of 75.75% with the RdRp of Fusarium redolens polymycovirus 1. Phylogenetic analysis based on the RdRp sequence clustered FoPmV1 robustly within the family Polymycoviridae. Overall, this research represents the first report of a polymycovirus infecting F. oxysporum.
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7. Enhanced induction of inflammatory cytokines in human lung cells infected with highly pathogenic recent H5N1/2024 avian influenza virus.
PMID:日期:2026-09-14Highly pathogenic (HP) H5N1 avian influenza viruses are zoonotic pathogens and are endemic in wild birds and poultry around the world. Pro-inflammatory cytokines are supposed to contribute to the severe clinical symptoms in infected animals and humans. In this study, we determined the infectivity and cytokine induction in primary human lung cells infected with HP H5N1 avian influenza viruses and seasonal H3N2 influenza viruses. MDCK cells were used to measure viral titers in the supernatants of infected human lung cells by log10TCID50/ml. Quantitative real-time PCR was used to detect the induction of cytokine genes using mRNA from the infected primary human lung cells. HP H5N1 avian influenza viruses induced higher viral titers than seasonal H3N2 influenza viruses in supernatants of primary human lung cells. In addition, HP H5N1avian influenza viruses induced the genes of pro-inflammatory cytokines such as IL-23, IL-1α, IL-1β, IL-6, and IL-8 at significantly higher levels than seasonal H3N2 influenza viruses in primary human lung cells. Our data suggest that the high viral replication and the high induction of pro-inflammatory cytokines in the human lung cells infected with HP H5N1 avian influenza viruses may enhance pathogenicity in the infected humans as compared to seasonal H3N2 influenza viruses.
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8. {"_":"Immunogenicity of a Vero cell-adapted Japanese encephalitis attenuated virus strain SA14-14-2 VC.","sub":["6"]}
PMID:日期:2026-09-11To develop a Vero cell-adapted Japanese encephalitis (JE) SA14-14-2 strain for the production of JE SA14-14-2 live attenuated vaccine (LAV) using Vero cells as the substrate, the SA14-14-2 PHKC strain was adapted to Vero cells and subjected to plaque purification followed by clone selection. A highly attenuated strain, SA14-14-2 VC, was isolated, whose phenotypic and genotypic characteristics were highly consistent with those of its parental strain SA14-14-2 PHKC. Immunogenicity assays demonstrated that the VC strain exhibited comparable immunogenicity to the parental SA14-14-2 strain. Under the experimental conditions used in this study, the VC strain induced stronger neutralizing antibody (NAb) responses, protection against intraperitoneal (i.p.) or intracerebral (i.c.) challenge with wild-type JE virus (JEV), viremia inhibition, and cellular immune responses than the chimeric JE-CV vaccine. Collectively, these findings indicate that the SA14-14-2 VC retains the immunogenic properties of the parental SA14-14-2 strain after adaptation to Vero cells and warrants further preclinical evaluation before its potential use in Vero cell-based JE vaccine development.
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9. Complete genome sequence of a novel alternavirus infecting Fusarium falciforme.
PMID:日期:2026-09-11We present the complete genome sequence of a novel alternavirus, tentatively named "Fusarium falciforme alternavirus 1 (FfAV1)", isolated from Fusarium falciforme. The host, F. falciforme strain Fod375, was isolated from a soil sample in Spain in 2012 and was found to be infected with a virus containing a tetra-segmented double-stranded (ds) RNA genome. The genome segments, designated as dsRNA1 (3529 bp), dsRNA2 (2641 bp), dsRNA3 (2459 bp), and dsRNA4 (1471 bp), each possess a single open reading frame (ORF). The protein predicted from dsRNA1 contains the typical domains of an RNA-dependent RNA polymerase (RdRP) homologous to those of previously reported alternaviruses, while the protein predicted from dsRNA3 shows homology to alternavirus capsid proteins. The proteins encoded by dsRNA2 and dsRNA4 are of unknown function. All predicted proteins exhibited the highest sequence identity with their counterparts in Hebei alternavirus and Marquandomyces marquandii alternavirus 1. Phylogenetic analysis supported the placement of this FfAV1 isolate within the genus Alternavirus. Considering these results, we propose that FfAV1, along with the two closely related unassigned alternaviruses, represents a new species within the genus.
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10. Isolation and characterization of a novel Schitoviridae phage VipHU7 that infects Vibrio parahaemolyticus.
PMID:日期:2026-09-11In recent years, various bacteriophages that infect Vibrio spp. have been isolated and characterized. However, many characteristics concerning their infection mechanisms remain unknown. Here, we isolated and characterized a novel phage, VipHU7, that infects Vibrio parahaemolyticus. The morphology of VipHU7 was examined using transmission electron microscopy, which demonstrated that it has a short, noncontractile tail characteristic of podoviruses. VipHU7 formed clear plaques with halo zones on a bacterial lawn of V. parahaemolyticus MFS 1101, and host range analysis revealed that it had a limited host range. Analysis of the propagation and one-step growth curve of VipHU7 in liquid medium indicated that its replication rate increases in the presence of divalent cations, which did not affect its adsorption. Genome sequencing revealed that the VipHU7 genome is 76,454 bp long, with a 38.48% GC content and 112 predicted open reading frames. VipHU7 has a genomic structure similar to that of other Varunavirus phages that infect Vibrio spp. VIRIDIC analysis showed that the intergenomic similarity between VipHU7 and Vibrio phage BUCT194 was 83.7%, indicating that VipHU7 is a novel species belonging to the family Schitoviridae and genus Varunavirus.