急性缺血性卒中远程缺血预处理后血浆的定量蛋白质组学分析
Quantitative proteomic analysis of plasma after remote ischemic conditioning in acute ischemic stroke.
摘要(中文译文)
远程缺血预处理（RIC）已被证明对急性缺血性卒中有效，许多生物标志物已被研究；然而，很少有研究同时检测到几种生物标志物并观察其动态变化。我们的目的是确定其变化与卒中后RIC相关的血清生物标志物。这是对RICAMIS试验的探索性分析，在该试验中，收集了血清样本的患者被纳入并分为治疗组。在入院时、随机分组后3天和出院时收集血清样本。确定了组间变化显著不同的血清生物标志物。在25名入选患者中，9名患者被分配到RIC组，16名患者被分到对照组。与对照组相比，RIC组的9种血清生物标志物在入院时与随机分组后3天以及入院时与出院时发生了显著变化。这些包括神经生物标志物（V型质子ATP酶亚基G3和分泌素-3）、血管相关生物标志物、炎症生物标志物和胰蛋白酶-1。这是第一项通过定量蛋白质组学分析确定急性缺血性卒中患者RIC治疗后血清生物标志物变化的研究，这将为未来临床试验中选择靶人群提供指导。已鉴定的生物标志物与RIC疗效的关系值得进一步研究。
Abstract
Remote ischemic conditioning (RIC) has been demonstrated to be effective in acute ischemic stroke, and numerous biomarkers have been investigated; however, few studies have simultaneously detected several biomarkers and observed their dynamic changes. We aimed to identify serum biomarkers whose changes are associated with RIC following stroke. This was an exploratory analysis of the RICAMIS trial, in which patients with serum samples collected were enrolled and divided into treatment groups. Serum samples were collected at admission, 3 days after randomization, and at discharge. Serum biomarkers with significantly different changes between groups were identified. Of the 25 enrolled patients, 9 patients were assigned to the RIC group, while 16 patients were assigned to the Control group. Compared with Control group, 9 serum biomarkers were found to significantlychange at admission versus 3 days after randomization, as well as at admission versus discharge in the RIC group. These included neurological biomarkers (V-type proton ATPase subunit G3 and secretogranin-3), vascular-related biomarkers (angiopoietin-related protein 6, Histone-lysine N-methyltransferase 2D, keratin type I cytoskeletal 18, and mitogen-activated protein kinase 3), inflammatory biomarkers (macrophage receptor and Nonspecific cytotoxic cell receptor protein-1), and trypsin-1. This is the first study to identify changes in serum biomarkers after RIC treatment in patients with acute ischemic stroke by quantitative proteomic analysis, which will provide the guidance for selecting target population in future clinical trials. The relationship of identified biomarkers with RIC efficacy warrants further investigation.
如何引用
AMA
Yu Cui, Fei Liu, Ji-Ru Cai, Hui-Sheng Chen. Quantitative proteomic analysis of plasma after remote ischemic conditioning in acute ischemic stroke.. Scientific reports. 2026; doi:10.1038/s41598-026-36968-3.
APA
Yu Cui, Fei Liu, Ji-Ru Cai, Hui-Sheng Chen (2026). Quantitative proteomic analysis of plasma after remote ischemic conditioning in acute ischemic stroke.. Scientific reports. https://doi.org/10.1038/s41598-026-36968-3
GB/T 7714
Yu Cui, Fei Liu, Ji-Ru Cai, Hui-Sheng Chen. Quantitative proteomic analysis of plasma after remote ischemic conditioning in acute ischemic stroke.[J]. Scientific reports, 2026 doi:10.1038/s41598-026-36968-3.
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