Transboundary and Emerging Diseases跨境与新发疾病
Transboundary and Emerging Diseases(英文缩写 TRANSBOUND EMERG DIS),ISSN 1865-1674,eISSN 1865-1682,中文译名:跨境与新发疾病 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.521 | Q1 |
| 2022 | 4.300 | Q1 |
| 2023 | 3.500 | Q1 |
| 2024 | 3.000 | Q1 |
| 2025 | 2.600 | Q1 |
Transboundary and Emerging Diseases 最新收录文献
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1. Virome Diversity and Zoonotic Risks of Haemaphysalis concinna Ticks in Northeastern China.
PMID:日期:2026-01-01Haemaphysalis concinna is a prevalent tick vector in Northeastern China, exhibiting a broad host range and the capacity to transmit over 40 human pathogens. Although numerous virome studies have investigated this tick species in Northeastern China, individual-level viral prevalence, coinfection dynamics, and the host spectra of the harbored pathogenic viruses remain poorly characterized. In this study, we conducted metatranscriptomic sequencing combined with TaqMan-based real-time quantitative PCR (RT-qPCR) validation on individual ticks to systematically profile the RNA virome of 112 adult H. concinna collected across Northeastern China. A total of 13 RNA viruses spanning six families and one unclassified group were identified, including four confirmed human pathogens, Tick-borne encephalitis virus (TBEV), Dabieshan tick virus (DBSTV), Songling virus (SGLV), and Wetland virus (WELV), alongside one potential human pathogen, Ji'an nairovirus (JANV). Individual-tick RT-qPCR assays revealed an overall viral positivity rate of 46.4%, with prevalence for specific viruses ranging from 0.9% to 13.4%. Notably, multiviral coinfections were detected in 10.7% of ticks (8.9% harboring two viruses and 1.8% harboring three), predominantly involving nairoviruses and rhabdoviruses. Phylogenetic analyses further confirmed that local viral strains clustered closely with endemic pathogenic lineages. Furthermore, literature-based host spectrum analysis demonstrated that these pathogenic viruses possess extensive vertebrate host ranges. Our findings highlight extensive viral diversity, high infection rates, and frequent coinfections in H. concinna, underscoring its pivotal role in maintaining and transmitting emerging tick-borne viruses and emphasizing the heightened risk of zoonotic spillover in Northeastern China.
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2. Rapid Identification and Differential Detection of Infectious Bursal Disease Virus by an Innovative ARMS-qRT-PCR Assay.
PMID:日期:2026-01-01Infectious bursal disease (IBD), caused by IBD virus (IBDV), is a major threat to the poultry industry, with the cocirculation of multiple genotyping strains complicating the diagnosis and control. Since the differences of antigenicity among different genotyping and their differences with the commonly used vaccine strains, rapid and accurate identification and differential detection are crucial for surveillance and control of IBDV. In this study, a novel amplification refractory mutation system quantitative reverse transcription PCR (ARMS-qRT-PCR) assay, for the rapid differential diagnosis of very virulent IBDV (vvIBDV), novel variant IBDV (nvIBDV), and attenuated IBDV (attIBDV) strains that are now popularly cocirculating in China and some other countries, was developed. By combining ARMS technology with qRT-PCR, this method enables accurate differentiation of these genotypes, including the coinfected samples. The developed ARMS-qRT-PCR assay demonstrated high repeatability and specificity, with no cross-reactivity among the three genotyping or other common avian pathogens. The assay exhibited high sensitivity and excellent linear correlation, with detection limits of 65.6, 57.8, and 70.2 copies/μL for vvIBDV, nvIBDV, and attIBDV, respectively. It also showed results that were completely consistent with the sequencing validation in identifying single and coinfected field samples. Crucially, it successfully identified and quantified individual differential detection in complex dual and triple coinfection cases. This high-throughput, user-friendly method offers significant advantages over traditional techniques, making it an ideal tool for real-time diagnosis, epidemiological surveillance, and outbreak management of IBDV.
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3. Andrographolide Inhibits Rabies Virus Replication.
PMID:日期:2026-01-01Rabies is a fatal zoonotic disease caused by infection with the rabies virus (RABV). Anti-RABV serum or antibody is a unique treatment for patients infected by RABV. However, the mortality is almost 100% in the underdeveloped regions due to the high price and the high cost of transportation and preservation of anti-RABV serum or antibody. Therefore, it is urgently needed to develop novel therapies that can effectively fight against RABV infection. Andrographolide (Andro) is a labdane diterpenoid isolated from Andrographis paniculate, which has been used to treat bacterial and viral infections for decades. In this study, we found that Andro dose-dependently inhibited RABV replication in host cells, with a half-maximal effective concentration (EC) of slightly less than 60 μmol/L. The antiviral effects of Andro were independent of viral strains and cell lines. Pretreatment of RABV with Andro in test tubes for 1 h did not affect virus replication in host cells, implying that Andro did not directly reduce RABV viability. Of interest, Andro treatment showed no influence on either RABV binding to cells or RABV invasion of cells. Also, Andro treatment downregulated the expression of RABV genomic RNA and structural genes in the cells. To investigate the therapeutic efficacy of Andro in vivo, mice were administered Andro via intraperitoneal (i.p.) injection or intragastric (i.g.) gavage. Neither the administration route nor the dosage significantly affected body weight. Notably, Andro protected mice against a lethal challenge with RABV strain challenge virus standard 11 (CVS-11). Specifically, i.p. injection of 100 mg/kg and 1 mg/kg Andro increased survival rates from 20% to 60% and 80%, respectively, while i.g. gavage of 250 mg/kg Andro elevated the survival rate from 30% to 60%. Correspondingly, RABV viral loads in mouse brain tissues were also reduced. The results suggest that Andro has great potential as a novel anti-RABV agent for the treatment of rabies.
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4. Longitudinal Molecular Epidemiology and Genomic Evolution of Porcine Reproductive and Respiratory Syndrome Virus in a Large-Scale Swine Production System in Northern China, 2023-2026.
PMID:日期:2026-01-01Porcine reproductive and respiratory syndrome virus (PRRSV) remains a major threat to swine production because of its persistent circulation, genetic diversity, and frequent recombination. However, longitudinal molecular surveillance within large-scale integrated production systems remains limited. In this study, we investigated the epidemiological and genomic characteristics of PRRSV in a large-scale swine production system in Chifeng, Inner Mongolia, northern China, from January 2023 to May 2026. A total of 380,050 PRRSV nucleic acid testing records were analyzed, and 36,474 were positive, yielding an overall positivity rate of 9.60%. PRRSV detection showed marked temporal fluctuations, with increased positivity from late 2025 to early 2026, and substantial heterogeneity among farms. Nursery pigs showed the highest positivity rate among production stages, followed by finishing pigs and gilts, whereas boars had the lowest positivity. Among sample types, castration/tail-docking fluid, tongue-tip fluid, and tissue/organ samples had relatively high positivity rates. ORF5-based phylogenetic analysis revealed that all the study-derived sequences belonged to PRRSV-2, with sublineage 1.8 accounting for 53.74% and sublineage 1.5 accounting for 34.69%, indicating the predominance of lineage 1 strains. Whole-genome phylogenetic analysis of the 11 successfully assembled PRRSV genomes confirmed that they were NADC30-like PRRSVs and carried the characteristic 111 + 1 + 19 discontinuous deletion pattern in Nsp2. GP5 amino acid alignment revealed substitutions in antigenically relevant regions, including epitope A, epitope B, and predicted glycosylation-related sites. Recombination analysis revealed potential recombination events involving lineage 8-related fragments in CF-k141-31, CF-k141-560, and CF-k141-2190. These findings demonstrate that PRRSV circulation in this production system is characterized by temporal clustering, farm-level heterogeneity, lineage 1 predominance, and genomic diversification of NADC30-like strains. These results support the integration of routine real-time reverse transcription quantitative PCR (RT-qPCR) surveillance, ORF5 sequencing, and periodic whole-genome sequencing for targeted PRRSV monitoring and control in large-scale swine production systems.
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5. Field-Collected Belgian Culex pipiens and Culex torrentium Mosquitoes are Competent for West Nile Virus Under Different Temperature Conditions.
PMID:日期:2026-01-01Belgium detected West Nile virus (WNV) for the first time on its territory in August 2025 during a monitoring of wild birds. To support risk assessments, we determined the vector competence of field-collected Belgian Culex pipiens and Culex torrentium mosquitoes, which are considered important WNV vectors. Females fed on a WNV-spiked (lineage 1 Israel 98 strain) blood meal were kept for 14 days at (i) a constant 25°C, (ii) a 25/15°C day/night gradient reflecting current Belgian summers, or (iii) a 25/20°C gradient simulating a climate change scenario. After the 14-day incubation period, abdomens, legs/wings/head, and saliva were collected and tested in RT-qPCR to determine the infection, dissemination, and transmission efficiencies (TEs). Observed infection efficiencies (IEs) (25.5%, 32.7%, and 34.3%) did not significantly differ between temperature conditions, and mostly mosquitoes with the lowest viral loads were dissemination-positive. The dissemination efficiency (DE) at 25/15°C (28.6%) was higher than in both other conditions and statistically significant compared to 25/20°C (10.4%), indicating that WNV more easily passes the midgut barrier under current Belgian summer temperatures. The entry of WNV into the salivary glands did not seem to be altered by temperature, as no significant differences were observed between the obtained TEs (3.9%, 8.2%, and 1.5% at 25°C, 25/15°C, and 25/20°C, respectively). By performing virus isolations on saliva, we proved that Culex pipiens is a competent vector for WNV at 25 and 25/15°C, while this was not confirmed at 25/20°C. Notably, a small percentage of the field-collected mosquitoes in this study were identified as Culex torrentium (7%), which also showed high competence for WNV. To conclude, our results suggest that field-collected Belgian Culex pipiens mosquitoes are competent vectors for WNV at 25°C and under current Belgian temperatures, emphasizing their potential to contribute to local WNV circulation and the importance of ongoing surveillance in birds, horses, and mosquitoes.
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6. An Exploratory One Health Investigation of Mycobacterium tuberculosis Complex at the Camel-Human Interface in Abu Dhabi Emirate, United Arab Emirates.
PMID:日期:2026-01-01This exploratory cross-sectional One Health study characterized LIODetect TB-ST antibody reactivity in dromedary camels and abattoir workers in Abu Dhabi Emirate and investigated tuberculosis-compatible lesions in slaughtered camels. Sera from 356 camels sampled from farms and livestock holdings (izbas), 393 camels presented for slaughter at Al Bawadi Abattoir, and 86 abattoir workers were screened with LIODetect TB-ST. All 25 LIODetect-reactive slaughtered camels underwent detailed postmortem examination; nonreactive slaughtered camels were not examined postmortem. Lesions identified in two reactive camels were evaluated by histopathology, Ziehl-Neelsen staining, and IS6110 polymerase chain reaction (PCR). LIODetect reactivity was detected in 33/356 field camels (9.3%, 95% CI: 6.5%-12.8%), 25/393 slaughtered camels (6.4%, 95% CI: 4.2%-9.2%), and 3/86 workers (3.5%, 95% CI: 0.7%-9.9%). No recorded characteristic was associated with reactivity among field camels. Among slaughtered camels, age group and body condition showed exploratory statistical associations with reactivity, but category-specific estimates were imprecise. Two of the 25 reactive slaughtered camels (8.0%) had granulomatous lymphadenitis with acid-fast bacilli, and lesion tissue from both animals contained IS6110-detectable Mycobacterium tuberculosis complex (MTBC) DNA. LIODetect TB-ST has not been validated for prevalence estimation in dromedaries; therefore, these proportions describe assay reactivity rather than true infection prevalence. Because postmortem examination was restricted to reactive animals, the 2/25 finding cannot estimate lesion prevalence, false-negative frequency, or positive predictive value. The three reactive workers represent an occupational-health surveillance signal, not evidence of active tuberculosis or camel-to-human transmission. Systematic meat inspection, validated camel diagnostics, culture and species-level molecular characterization, traceable animal movements, and linked veterinary and occupational-health surveillance are needed.
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7. Characterization and Evolutionary Analysis of Type E4 Bovine Enterovirus From Clinical Cattle With Diarrhea in Inner Mongolia, China.
PMID:日期:2026-01-01Bovine enterovirus (BEV) is associated with respiratory, intestinal, and reproductive disorders in cattle, but its evolutionary characteristics and pathogenic potential remain insufficiently understood. In this study, a BEV strain was successfully isolated from a diarrheic fecal sample collected from an 8-month-old Holstein calf and was designated BEV NM2407. The virus replicated stably in Madin-Darby bovine kidney (MDBK) cells, reaching a titer of 10 TCID/mL. Its complete genome was 7414 nt in length, and phylogenetic analysis classified the isolate as EV-E4. Recombination analysis identified two putative breakpoints at 1950 and 5850 nt, and the resulting genomic regions showed the highest similarity to EV-C- and EV-D-related reference lineages. VP1 analysis revealed a relatively conserved β-barrel core, whereas the major variations were concentrated in the surface-exposed BC, DE, and GH loops. Experimental infection showed that BEV NM2407 was able to infect calves and induce mild clinical signs. Viral RNA was detected in nasal and rectal swabs as well as multiple tissues, with relatively higher levels in the trachea, lung, cecum, and rectum. Gross pathological changes were mainly characterized by intestinal hyperemia/congestion, most prominently in the jejunum. Histopathological examination revealed epithelial necrosis in the jejunum and rectum, together with lymphocyte depletion and necrosis in ileal lymphoid follicles. Overall, BEV NM2407 demonstrated infectivity in calves under experimental conditions. The observed viral shedding, tissue distribution, and localized pathological lesions provide further evidence of the pathogenic potential of BEV, expand current understanding of its molecular evolution and biological characteristics, and provide a basis for further investigations into the pathogenesis of BEV infection in cattle.
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8. A Natural Enzyme GOx-MOF Cascade Complex Killing Zoonotic Parasite Trichinella spiralis by Energy Utilization Limitation and Generated Free Radicals.
PMID:日期:2026-01-01Nanozymes offer promising alternatives to natural enzymes due to enhanced stability, tunable activity, and cost efficiency, demonstrating therapeutic potential against tumors, infections, and inflammatory disorders. Trichinella spiralis is an important pathogen, which globally prevalent foodborne zoonotic parasite. Chemical drug is commonly used to kill T. spiralis, but there are risks of chemical abuse and drug resistance. Here, we engineered MPG(Fe), a cascade nanozyme formed by covalently conjugating glucose oxidase (GOx) to a metal-organic framework-Fe nanozyme (MOF-525[Fe]) nanozyme via the bifunctional PEG linker. In vitro, MPG(Fe) depleted glucose through GOx-catalyzed conversion to gluconic acid and HO, while MOF-525(Fe) subsequently transformed HO into cytotoxic hydroxyl radicals (•OH). This dual-action mechanism dose-dependently killed intestinal-stage adult worms and newborn larvae (NBL) of T. spiralis. In a BALB/c mouse model of intestinal T. spiralis infection, high-dose MPG(Fe) reduced the survival of intestinal adult worms to 3.53% of the control value through the synergistic actions of glucose deprivation and hydroxyl radical (•OH)-mediated oxidative damage. Critically, MPG(Fe) exhibited no tissue toxicity in uninfected mice. Our findings establish enzyme-mimetic nanozymes as a novel therapeutic platform against zoonotic helminthiases.
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9. Live Attenuated Vaccines for African Swine Fever: Perspectives on Their Use and Strategic Directions.
PMID:日期:2026-01-01African swine fever (ASF) continues to cause substantial economic and production losses in affected countries. Although biosecurity and conventional control measures remain essential, the challenges of ASF control underscore the need for safe and effective vaccines as additional tools, particularly in endemic settings. Live attenuated vaccines (LAVs) are the most advanced ASF vaccine platform, providing the most consistent protection to date and remaining the only ASF vaccines to reach commercial deployment. Field experience with licensed products has shown encouraging safety, immunogenicity, and disease-control outcomes. However, the introduction and spread of recombinant genotype I/II strains may reduce vaccine effectiveness and contribute to limited vaccine uptake. Together with the detection of vaccine-like variants of uncertain origin, these issues have generated contrasting perspectives: a precautionary view focused on risks associated with replication-competent vaccine viruses, and a benefit-risk view emphasizing the consequences of continued ASFV circulation without effective vaccination. This review contrasts these perspectives, draws lessons from other live vaccines, and frames the responsible use of ASF LAVs within a benefit-risk approach. Experience with human and veterinary LAVs shows that persistence, transmission, genetic instability, reversion to virulence, and recombination are inherent biosafety concerns of LAVs, yet such vaccines remain important when alternative platforms do not provide equivalent protection. Their use therefore requires identifying, monitoring and minimizing residual risks in relation to expected benefits. Accordingly, ASF LAVs should be avoided in ASF-free regions but may be justified in endemic settings when licensed products are used within coordinated programs integrating adequate coverage, biosecurity, surveillance, and molecular monitoring. Future vaccine development should focus on improving safety in growing pigs and breeding animals, broadening protection against emerging ASFV strains, validating DIVA tools and developing safer next-generation platforms, while continued reassessment ensures that vaccine use remains appropriate to epidemiological conditions, emerging evidence, and disease-control needs.
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10. Early African Swine Fever Dynamics in Wild Boar After Reemergence in Catalonia, Spain: Surveillance-Based Estimates of Initial Spread.
PMID:日期:2026-01-01African swine fever (ASF) reemerged in free-ranging wild boar in Catalonia, northeastern Spain, in November 2025, representing the first detection in Spain since 1994. We described the initial outbreak phase using surveillance data collected from 25 November 2025 to 23 February 2026. ASF virus (ASFV) positivity by real-time quantitative polymerase chain reaction (qPCR) was estimated overall and by surveillance stream; mortality timing was reconstructed probabilistically from estimated death-date intervals; and early front displacement was analyzed using leading-edge cumulative minimum convex polygons (MCPs), linear and generalized additive models (GAMs), segmented regression, and directional modeling. Among 1483 wild boars with definitive qPCR results, 201 were ASFV qPCR-positive, giving an overall qPCR positivity of 13.6% (95% confidence interval [CI] 11.9-15.4). Positivity was substantially higher in enhanced passive than in active surveillance (28.2% vs. 0.9%; positivity ratio [PR] 32.11, 95% CI 15.21-67.78; p < 0.001). A probabilistic cumulative mortality curve based on 198 positive carcasses indicated that, by the date of the first detected carcass, expected cumulative mortality had already reached 25.8 deaths (13% of reconstructed total mortality). Leading-edge analysis estimated an apparent mean radial front-displacement rate of 0.042 km/day (95% CI 0.029-0.055). The surveillance-derived distance-time relationship was nonlinear, indicating that the apparent front-displacement rate varied over the study period, with a segmented-regression breakpoint at 119 days and higher apparent front-displacement thereafter (0.106 vs. 0.021 km/day). The exploratory directional analysis indicated the fastest predicted advance toward the South-Southwest sector. These surveillance-derived estimates describe the observed leading edge under the surveillance and control conditions operating during the study period and should not be interpreted as direct estimates of ASFV transmission speed. These findings support an adaptive, risk-based response that prioritizes carcass search and removal and incorporates directional spread information to refine surveillance and zoning as the outbreak evolves. The analytical framework, combining reconstruction of estimated dates of death with simple descriptors of early epidemic spread, may support early epidemiological assessment of ASF incursions in other newly affected settings.