BRAZILIAN JOURNAL OF MICROBIOLOGY巴西微生物学杂志
BRAZILIAN JOURNAL OF MICROBIOLOGY(英文缩写 BRAZ J MICROBIOL),ISSN 1517-8382,eISSN 1678-4405,中文译名:巴西微生物学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.214 | Q4 |
| 2022 | 2.200 | Q4 |
| 2023 | 2.100 | Q3 |
| 2024 | 1.900 | Q4 |
| 2025 | 2.100 | Q4 |
BRAZILIAN JOURNAL OF MICROBIOLOGY 最新收录文献
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1. Molecular types, clinical profile, and exploratory virulence-associated phenotypes of clinical Cryptococcus isolates from Amazonas, Brazil.
PMID:期刊:日期:2026-09-30Cryptococcosis remains a severe systemic mycosis, especially among people living with HIV/AIDS, and culture-based molecular data from Amazonas remain limited. The aim was to identify which Cryptococcus molecular types circulated among clinical isolates from Amazonas between 2020 and 2024; what were the main clinical characteristics and outcomes of patients infected with Cryptococcus neoformans molecular type VNI and Cryptococcus gattii molecular type VGII; and whether these molecular types differed in selected susceptibility-related and virulence-associated phenotypes. We analyzed 70 culture-confirmed clinical isolates from patients treated at the Dr. Heitor Vieira Dourado Tropical Medicine Foundation, Manaus, Brazil. Molecular types were assigned by URA5 PCR-RFLP using reference strains. Demographic and clinical variables were retrieved from medical records. A stratified 30-isolate subset comprising 24 VNI and 6 VGII isolates was evaluated for phospholipase activity and melanization. Fluconazole MICs were analyzed for the complete 70-isolate dataset and were determined by broth microdilution according to CLSI M27-A4. VNI predominated (64/70; 91.4%), whereas VGII accounted for 6/70 isolates (8.6%). The series was predominantly composed of men, young adults, and HIV-positive patients. HIV infection was more frequent among VNI cases, whereas VGII included proportionally more HIV-negative patients. In-hospital death occurred in 17/70 patients (24.3%). Fluconazole MICs, phospholipase activity, and melanization were broadly comparable between VNI and VGII, although the small VGII subgroup precluded robust genotype-associated inference. VNI was the dominant molecular type in this severe reference-center cryptococcosis series from Amazonas. Phenotypic findings should be interpreted as exploratory and hypothesis-generating.
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2. Bacilli the most important biocontrol agent in Brazilian agriculture: from mechanisms of action to market adoption.
PMID:期刊:日期:2026-09-28Bacilli have become one of the most commercially significant groups of microorganisms used in agricultural biocontrol products in Brazil. Their ability to form endospores and produce a wide range of metabolites allows them to control plant diseases, promote plant growth, and alleviate stress, supporting their extensive adoption in crop protection systems. This study analyzes the Brazilian market for Bacillus-based products used in plant disease management and summarizes their mechanisms of action. Data on commercial registrations, active strains, product formulations, and target diseases were compiled from Brazilian national regulatory databases. Market indicators, such as potential area treated (PAT) and turnover, were estimated using structured agricultural surveys conducted across Brazil during the 2019/2020 and 2024/2025 crop seasons, the latter encompassing more than 16,000 interviews with farmers and agricultural managers. By December/2025, 297 Bacillus-based products containing 126 strains were commercialized in Brazil, with Bacillus amyloliquefaciens, B. subtilis, B. velezensis, and B. pumilus being the most prominent species. These products are applied via seed treatment, soil drenching, furrow, and foliar spraying to manage fungal, bacterial, and nematode diseases. During the analyzed period, PAT increased from 7.5 to 37.2 million hectares (391% growth) and turnover increased from US$114.31 to US$403.17 million (252% growth). Currently, Bacillus-based products accounted for approximately 48% of the Brazilian biological control market, highlighting the prevalence of this genus in the sector. Overall, these results demonstrate how advances in microbial biotechnology, regulatory development, and farmer adoption have positioned Bacillus spp. as key components of the rapidly expanding biocontrol industry.
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3. Organic acid-associated antimicrobial and antibiofilm activity of Lactobacillus rhamnosus ATCC 7469 against Acinetobacter baumannii and Staphylococcus aureus.
PMID:期刊:日期:2026-09-24Considering the relevance of biofilms in bacterial resistance and the need for innovative therapies against pathogenic microorganisms, alternative strategies using probiotics as antimicrobial agents have emerged. This study aimed to evaluate the antimicrobial and antibiofilm activities of the cell-free supernatant (CFS) of Lactobacillus rhamnosus ATCC 7469 against biofilm-forming strains of Acinetobacter baumannii and Staphylococcus aureus. The activity of CFS was evaluated against A. baumannii ATCC 19606, A. baumannii 301, S. aureus ATCC 12598, and S. aureus ATCC 29213. Antimicrobial activity against planktonic cells, inhibition of biofilm formation, and destruction of mature biofilms were assessed at different CFS dilutions. The corresponding concentrations of lactic and acetic acids were determined by high-performance liquid chromatography (HPLC). CFS showed high antimicrobial activity, with growth inhibition ranging from 88.26% to 100% at the two highest concentrations tested (197.8/108.7 and 107.0/54.5 mM lactic/acetic acid). At 57.8/28.3 mM, growth inhibition ranged from 60.05% to 81.65%, whereas at 27.2/13.5 mM it ranged from 21.12% to 41.03%. CFS also strongly inhibited biofilm formation, with inhibition rates ranging from 66.17% to 100%, whereas destruction of mature biofilms ranged from 24.75% to 88.11%. HPLC analysis demonstrated the presence of lactic and acetic acids in the CFS, with lactic acid being the predominant organic acid detected. Neutralization of the CFS to pH 7.0 markedly reduced its antimicrobial and antibiofilm activities, supporting an important contribution of acidity and acid-associated components to the observed effects. Overall, L. rhamnosus ATCC 7469 CFS exhibited substantial antimicrobial and antibiofilm activity against A. baumannii and S. aureus, supporting further investigation of Lactobacillus-derived CFS as a potential local antimicrobial strategy.
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4. Biocontrol of Staphylococcus aureus in dairy products using lytic bacteriophage UHP46: efficacy in milk, cheese, and yogurt.
4. 利用裂解性噬菌体UHP46对乳制品中金黄色葡萄球菌的生物防治：在牛奶、奶酪和酸奶中的效果PMID:期刊:日期:2026-09-24Staphylococcus aureus is a major public health concern in the dairy industry. It is a well-recognized pathogen responsible for bovine mastitis and raw milk contamination; certain strains produce heat-stable enterotoxin capable of causing staphylococcal food poisoning in humans. The emergence of multiple drug-resistant strains of S. aureus has rendered ineffective the use of antibiotics for control purposes. This study determined the antibacterial activity of lytic bacteriophage UHP46 against a single, previously characterized multidrug-resistant mastitis isolate, S. aureus S46, in three dairy matrices - milk, cheese and yogurt - under storage conditions representing refrigerated storage (4 °C), ambient conditions (25 °C) and a 37 °C mechanistic reference condition. UHP46 significantly reduced S. aureus S46 counts in milk, achieving reductions of 3.2 and 3.7 log₁₀ CFU/mL after 4 and 24 h at 4 °C, 2.1 and 3.1 log₁₀ CFU/mL after 4 and 24 h at 25 °C, and 1.8 log₁₀ CFU/mL after 3 h at 37 °C. The reduction at 37 °C was accompanied by an increase in phage titer from 7.2 to 8.8 log₁₀ PFU/mL. Application of UHP46 to cheese resulted in an immediate reduction of 0.6 log₁₀ CFU/g after 5 min of treatment at room temperature. The maximum reduction observed was 2.0 log₁₀ CFU/g at 24 h, with a sustained reduction of 1.9 log₁₀ CFU/g maintained through 96 h of refrigerated storage at 4 °C. In yogurt, net reductions on day 6 were 2.8, 2.7, and 1.6 log₁₀ CFU/g at 37 °C, 25 °C, and 4 °C, respectively. The findings indicate that UHP46 exhibits significant antibacterial activity against S. aureus S46 within the tested dairy matrices and under the evaluated storage conditions. However, as all challenge experiments utilized a single host strain, its efficacy against a broader panel of S. aureus, including methicillin-resistant S. aureus (MRSA), remains to be determined.
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5. Behavior of surface contamination by Salmonella spp. on broiler carcasses during slaughter stages.
PMID:期刊:日期:2026-09-23Salmonella spp. is a globally relevant pathogen frequently associated with foodborne infections and poultry products. This study aimed to evaluate the occurrence and quantification of Salmonella spp. during different stages of the broiler slaughter process and to assess the association of processing stages with reductions in contamination levels. The study was conducted in a federally inspected broiler slaughterhouse in Paraná, Brazil. A total of 2,100 analyses were performed on chicken carcasses collected at seven points along the slaughter line over 20 weeks. Salmonella detection followed the ISO 6579:2017 methodology, while quantification was performed using the miniaturized most probable number (mMPN) method with molecular confirmation by the invA gene. Subsequently, 211 isolates were serotyped, and 97 Salmonella Heidelberg isolates were analyzed by pulsed-field gel electrophoresis (PFGE). The highest occurrence of Salmonella spp. was observed at the pre-scalding stage (N = 236/78.66%). Significant reductions in Salmonella counts were observed after the scalding stage, after the post-evisceration critical control point for removal of visibly contaminated carcasses (CCP 1B), and after final washing before chilling (P < 0.05). Five serovars were identified, with Salmonella Heidelberg predominating (N = 193/91.5%). PFGE analysis revealed high genetic similarity (90%) among isolates, with highly similar profiles identified at different slaughter stages and sampling weeks. These findings demonstrate that Salmonella spp. was detected throughout the slaughter process, although reductions in contamination levels were observed at specific processing stages. In addition, the detection of highly similar isolates at different sampling points and weeks contributes to the understanding of Salmonella contamination dynamics in poultry slaughterhouses.
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6. Microbiological safety and antimicrobial resistance in cashew-based plant-based cheese analogues.
6. 基于腰果的植物基奶酪类似物的微生物安全性与抗菌药物耐药性PMID:期刊:日期:2026-09-23The demand for plant-based cheese analogues has increased in recent years, driven both by the growing adoption of vegan and vegetarian diets and by consumers seeking plant-based alternatives to animal-derived products. Their production relies on diverse raw materials and often incorporates fermentation processes involving molds, yeasts, and lactic acid bacteria (LAB). Within this context, ensuring food safety, including the antimicrobial resistance (AMR) aspects, is essential due to the complex microbial communities involved in fermentation. Therefore, this study investigated the presence of bacterial species of potential public health relevance and assessed AMR profiles and genes in plant-based cheese analogues acquired in Brazil. None of the samples tested positive for Salmonella spp., coagulase-positive Staphylococcus, or Escherichia coli, while Bacillus cereus was detected in one sample. Two atypical colonies recovered on selective media for Salmonella were identified as Cronobacter malonaticus and Delftia acidovorans. Most samples exhibited high mold and yeast counts, likely associated with fermentation and maturation processes. Among 176 LAB isolates, 11 species were identified by MALDI-TOF, and twenty representative strains were selected for antimicrobial susceptibility testing. Resistance was detected in a limited number of isolates, mainly to ciprofloxacin, tetracycline, and gentamicin, whereas C. malonaticus exhibited resistance to penicillin. Whole-genome sequencing was performed on Lactiplantibacillus plantarum, Levilactobacillus brevis, Leuconostoc mesenteroides, Enterococcus faecium, and C. malonaticus. A total of eight antimicrobial resistance genes were identified, four of which were located on plasmids in C. malonaticus and L. brevis. These findings suggest that microorganisms associated with plant-based cheese analogues may act as reservoirs of antimicrobial resistance determinants, highlighting the importance of safety assessment and microbiological surveillance of strains intended for technological or probiotic applications.
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7. Antimicrobial activity and chemical characterization of Algerian Thymus algeriensis essential oil incorporated in active packaging for chicken meat preservation.
PMID:期刊:日期:2026-09-21Thymus algeriensis (T. algeriensis), a North African thyme species, exhibits notable antimicrobial potential. This study evaluated the antibacterial activity of its essential oil (EO) and assessed its application in active cellophane packaging for chicken meat preservation. The EO was impregnated onto cellophane sheets and applied in situ on chicken thighs artificially contaminated with Escherichia coli ATCC 25,922 (E. coli) and stored at 5 ± 1 °C for 96 h. The EO chemical composition was determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Antimicrobial activity was assessed in vitro using disc-diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) assays against E. coli, Staphylococcus aureus ATCC 25,923 (S. aureus), Candida albicans ATCC 71,453 (C. albicans), and Aspergillus niger ATCC 16,404 (A. niger). Results showed a marked reduction in microbial load in EO-treated samples compared with controls, demonstrating the effectiveness of EO-based active packaging in inhibiting bacterial growth under refrigerated storage. These findings support the potential of T. algeriensis EO as a bioactive natural preservative for active food packaging systems to enhance food safety and extend shelf life.
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8. Industrial-scale optimization of fed-batch fermentation enhances biomass productivity of Pasteurella multocida B:2 for hemorrhagic septicemia vaccine.
PMID:期刊:日期:2026-09-18Hemorrhagic septicemia (HS), caused by Pasteurella multocida serotype B:2, remains a major transboundary disease of cattle and buffalo, particularly in tropical regions. Efficient vaccine production requires high-density biomass generation under controlled and scalable fermentation conditions. This study designed as a sequential process-development and optimization study aimed to optimize industrial-scale cultivation of P. multocida B:2 using a 150-L bioreactor system with S1-S5 samples (n = 5) representing independent fermentation runs from analytical measurements through media selection, fed-batch supplementation, pH control, and thermal preconditioning strategies. Comparative batch cultivation demonstrated superior biomass production in tryptose phosphate broth (TPB) compared to casein sucrose yeast (CSY) medium (OD600 1.57 vs 0.76; p < 0.001). Implementation of fed-batch supplementation significantly enhanced cell density, with a glucose-yeast extract-heart infusion formulation yielding the highest biomass (OD600 3.96; 1.98 × 10⁹ cells/mL) within 8 h of fermentation. Optimal growth was achieved at a controlled pH range of 7.30-7.35, which reduced fermentation time by approximately 33% compared to pH 7.40-7.45. Additionally, pre-heating the culture medium to 40°C prior to inoculation significantly increased biomass yield (p < 0.001). Overall, process intensification through optimized fed-batch strategy and pH control resulted in a > 2.5-fold increase in biomass productivity compared to conventional batch cultivation. These findings provide a scalable and cost-effective fermentation framework for improved commercial production of HS vaccine antigen.
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9. Postbiotic potential of heat-inactivated Limosilactobacillus reuteri and its cell-free supernatant in protecting intestinal health of zebrafish.
PMID:期刊:日期:2026-09-14Postbiotics are non-viable substances derived from probiotics, offering advantages including high stability, strong safety profiles, and broad-spectrum effects. The present study aims to investigate the impact of Limosilactobacillus reuteri postbiotics on the intestinal health of zebrafish. Zebrafish were fed basal diet supplemented with either 10⁷ CFU/g heat-inactivated L. reuteri (hLR) or corresponding cell-free L. reuteri supernatant (LRs) for 14 days. Compared to the control group (CT), villus height in the zebrafish foregut and midgut were increased in LRs group. In addition, intestinal superoxide dismutase activity was significantly enhanced in hLR group and LRs group. Gut microbiota analysis revealed that both the hLR and LRs groups exhibited increased OTUs richness, altered microbial community structure at the phylum and genus levels, and reduced abundance of potential pathogenic bacteria in the gut community structure. Moreover, transcriptome analysis indicated that the functional enrichment of differentially expressed genes in the hLR group was primarily focused on immune regulation, while that in the LRs group was mainly concentrated on cell cycle regulation. The present study found that different postbiotic components of L. reuteri exerted distinct effects on zebrafish intestinal health, providing crucial evidence for the application of L. reuteri postbiotics as an effective feed additive in aquaculture.
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10. Screening and ARTP mutagenesis-based breeding of high-cellulase-producing strains.
PMID:期刊:日期:2026-09-14While lignocellulose is an abundant renewable resource, the discovery of microorganisms capable of degrading lignocellulose is important for its full utilization. In this study, 26 soil samples were collected from Jigongshan National Nature Reserve, Xinyang, China. The Congo red staining method was used to isolate strains capable of degrading lignocellulose from the soil samples. A strain W-6-1, isolated from the soil samples was able to effectively degrade lignocellulose, with the filter paper activity (FPA) in the fermentation broth reaching 3.34 ± 0.32 U/mL. Integrating morphological traits, 16S rRNA phylogeny and physiological-biochemical assays, the isolate was identified as Bacillus sp. W-6-1. Three rounds of atmospheric and room-temperature plasma (ARTP) mutagenesis were employed to further enhance the lignocellulose-degrading ability of this strain. The best mutant (strain M3-35), which was selected from the surviving colonies, achieved an FPA of 6.11 ± 0.27 U/mL, representing an 83% increase over the wild-type strain W-6-1. Moreover, after multiple successive transfers, the FPA of strain M3-35 remained stable. The mutant M3-35 generated in this work provides a promising laboratory-derived candidate for exploring lignocellulose bioconversion.