BIOTECHNOLOGY AND BIOENGINEERING生物技术与生物工程
BIOTECHNOLOGY AND BIOENGINEERING(英文缩写 BIOTECHNOL BIOENG),ISSN 0006-3592,eISSN 1097-0290,中文译名:生物技术与生物工程 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.395 | Q2 |
| 2022 | 3.800 | Q2 |
| 2023 | 3.500 | Q2 |
| 2024 | 3.600 | Q2 |
| 2025 | 3.900 | Q2 |
BIOTECHNOLOGY AND BIOENGINEERING 最新收录文献
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1. Highly Efficient Enzymatic Catalytic Transformation for the Synthesis of Rare Ginsenoside Compound K (CK) and Its Bioactivity Study.
PMID:日期:2026-10-01As a principal metabolite of ginsenosides, compound K (CK) exhibits remarkable antioxidant, anti-inflammatory, antidiabetic, and anticancer bioactivities. However, CK is naturally scarce in ginseng and its industrial application is limited by the lack of efficient deglycosylating enzymes for large-scale CK bioproduction. Here, we report a synthetic biology-driven approach for high-yield CK production via recombinant β-glycosidase-mediated bioconversion of the polar ginsenoside Rb1. The β‑glycosidase-encoding gene was introduced into Escherichia coli BL21 (DE3), and the resulting engineered strain produced 2.1 g/L β‑glycosidase in shake flasks and 10.1 g/L in a 5-L fermentor. The crude β-glycosidase exhibited excellent enzymatic activity; at a concentration of 1.5 g/L, it converted 95% of the Rb1 substrate (initial concentration 10 g/L) to CK within 4 h. In vitro studies demonstrated CK's high cytocompatibility and potent anti-aging effects, including suppression of reactive oxygen species (via upregulation of superoxide dismutase, catalase, and glutathione peroxidase), enhanced collagen I, Ⅲ and elastin synthesis, and matrix metalloproteinase-1 inhibition. These findings highlight CK as a promising candidate for anti-wrinkle formulations, supported by the feasibility of scalable bioproduction.
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2. Challenges and Future Directives of Synthetic Biology in Engineering Plant-Microbe Partnerships for Sustainable Agriculture.
PMID:日期:2026-10-01Synthetic biology has recently proven to be a valuable tool for enhancing agriculture even in the face of environmental and biological stresses. Understanding the challenges that militate synthetic biology will assist in ensuring safe, stable, and scalable crop production. Thus, we examined the challenges limiting synthetic biology applications in engineering plant-microbe partnerships and highlighted future research directions. Ecological, biological, technical, and regulatory barriers to synthetic biology-driven plant-microbe engineering are the challenges examined in this review. Profound insight into these challenges will lead to a shift toward systems-level approaches that integrate multiomics analyses, predictive modeling, and framework-responsive genetic designs. To completely translate synthetic biology from the laboratory to the field, improved delivery methods, monitoring strategies, and harmonized regulatory frameworks should be encouraged. In addition, the development of robust and controllable microbial chassis should be emphasized.
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3. Polycationic Peptide Engineering of Phage Endolysins Expands Host Range and Enhances Antibacterial and Antibiofilm Activities Against Bacillus Species.
PMID:日期:2026-10-01The exponential rise in antimicrobial resistance has highlighted the urgent need for the development of alternative antibacterial strategies, including phage-derived and engineered protein agents such as endolysins and artilysins. In this study, we report the rational design, expression, and functional characterization of novel artilysins derived from P19_358 lysin, a glycoside hydrolase family 24 enzyme. The native P19_358 lysin exhibits limited antibacterial activity, primarily against Bacillus subtilis, and requires EDTA pre-treatment to act against Gram-negative bacteria. To overcome this limitation, C-terminal fusion constructs were engineered using polycationic peptides, including a Cecropin A-derived peptide fragment and polycationic nonapeptide (PCNP), with and without flexible glycine-serine linkers. Structural and biophysical analyses confirmed that peptide fusion did not interfere with the native catalytic domain. Functional assays demonstrated that the engineered artilysins exhibited enhanced antibacterial activity and an expanded host range compared to the native enzyme. Among these constructs, Cecropin A fused artilysins (Art1 and Art2) showed the highest bactericidal activity, achieving up to ~5.4 log reductions in B. subtilis MTCC 121 and ~4.9 log reductions in B. pumilus MTCC 1640 and B. licheniformis MTCC 429 . Additionally, Art2 exhibited pronounced antibiofilm activity, significantly reducing biofilm formation across the tested Bacillus species. Stability studies revealed that Art2 possessed superior thermal, pH, and salinity tolerance compared to the native lysin, retaining activity across a broad range of environmental conditions. These findings demonstrate that rational artilysin engineering can substantially enhance antibacterial potency, spectrum, and stability while preserving enzymatic function. Notably, Art2 emerges as a promising candidate for further development as a robust antimicrobial agent with potential applications in food safety and clinical settings.
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4. Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.
4. 连接芯片上的组织和组学：生物医学研究的多维前沿PMID:日期:2026-10-01Organ-on-a-Chip (OOC) technology offers a powerful platform for replicating human tissue-specific microenvironments, thereby narrowing the translational gap between conventional biomedical models and actual human physiology. Concurrently, omics technologies deliver comprehensive molecular-level insights into biological systems. This review highlights the transformative potential of integrating OOC platforms with high-throughput omics methodologies. We systematically examine the classification, structural configurations, and engineering principles underlying OOC systems, alongside the defining attributes of key omics domains-genomics, transcriptomics, proteomics, and metabolomics. The convergence of dynamic OOC models with advanced omics technologies enables high-resolution, multi-dimensional analyses across numerous biomedical applications, including drug metabolism, disease mechanisms, environmental toxicity assessments, and host-microbiome interactions. This interdisciplinary integration is driving a paradigm shift in precision and translational medicine. However, several challenges remain to be addressed, such as the development of whole-organ mimetics, adaptation of sample collection techniques, and real-time artificial intelligence-based integration of biosensor data with multi-omics datasets. Addressing these hurdles will be vital for unlocking the full potential of this technological synergy in biomedical science.
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5. Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.
PMID:日期:2026-10-01The extracellular polymeric substances (EPS) matrix of microbial biofilms exhibits a complex structural heterogeneity that profoundly influences mass transport and metabolic activity. Conventional biofilm models typically assume a homogeneous matrix, thereby neglecting the localized transport resistance introduced by the bacterial capsule, a distinct, low-diffusivity polysaccharide layer surrounding individual cells. In this theoretical study, we develop a multiscale "cell-capsule" continuum model that represents the capsule as a concentric shell enveloping each microbial cell core within the bulk EPS. Utilizing a one-dimensional reaction-diffusion framework coupled with a geometric characterization of capsule spacing and thickness, we quantify how microscale architecture modulates oxygen transport in developing biofilms. Model simulations demonstrate that incorporating a discrete capsular phase introduces a pronounced "resistance-in-series" effect, reducing local oxygen availability by up to 70% compared to conventional homogeneous models. Furthermore, our analysis indicates that capsule thickness and matrix compaction jointly control the effective diffusivity and oxygen effectiveness factor within the biofilm. These results provide critical mechanistic insights into how microscale organization governs macroscale biofilm function, offering a new framework for integrating structural heterogeneity into multiscale biofilm simulations.
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6. Revisiting the Carbon Footprint of Single-Use Technologies in Biomanufacturing: A Bottom-Up Analysis Reveals a Paradigm Shift.
PMID:日期:2026-10-01The biopharmaceutical industry increasingly relies on single-use technologies (SUT) for operational flexibility. For a long time, SUT was considered more sustainable than stainless-steel (SST) systems due to water and energy savings. This study re-evaluates this paradigm via a detailed bottom-up analysis of the SUT carbon footprint at the 2000 L scale. Our analysis, based on two real-world facility case studies (a full-SUT and a hybrid-SUT model), shows that the CO2 footprint of SUT is significantly higher than previously assumed. This granular analysis, based on physical disassembly and updated "cradle-to-gate" factors, identifies filters and bags as key emission hotspots. A separate comparative analysis also shows that key SST process steps-favored by the progressive decarbonization of electricity grids-can now have a lower carbon footprint than their SUT counterparts. Our data demonstrates that an operationally optimized hybrid facility design, which combines SUT with SST, can significantly reduce plastic waste and associated emissions. These findings compel a reassessment of sustainability strategies in biopharmaceutical manufacturing and highlight the potential of fit-for-purpose hybrid models as an effective lever for reducing the ecological footprint.
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7. Rickettsial Diseases and Vaccines: Is There an Opportunity for Plant-Made Vaccine Technology?
PMID:日期:2026-10-01Rickettsiosis is a zoonotic disease caused by obligate intracellular bacteria of the family Rickettsiae, transmitted by arthropods. No commercial vaccine exists, despite the efficacy of experimental prototypes based on whole cells, DNAs, nanosystems, and recombinant antigens. Plants have been used as a platform for producing and delivering recombinant antigens, allowing the commercial vaccines for COVID-19 and Influenza. This technology can be used for recombinant Rickettsia vaccines for the target host, potentially for vector arthropods. These aspects are reviewed in this work, considering a brief immunobiology of Rickettsiae, experimental vaccines against Rickettsiosis, step-by-step genetic engineering in plants for recombinant antigen production and immunological assessment, representative successful examples of plant-made vaccines, and the analysis of the potential of plant-made vaccines to control arthropod-transmitted Rickettsiae. Perspectives in this field are offered, highlighting hurdles and opportunities.
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8. Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.
PMID:日期:2026-10-01The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum-free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m), pharmaceutical-grade amino acids and recombinant proteins remain major cost drivers. Techno-economic analyses suggest that replacing purified amino acids is essential to achieve price parity with conventional meat. Microbial hydrolysates, also referred to as extracts, offer a promising alternative, as these can supply complex mixtures of amino acids and peptides at lower cost and with greater scalability than chemically defined formulations. In particular, yeast and bacterial extracts combine high protein (and therefore high amino acid) content, rapid growth, and established industrial-scale production. Comparative analyses indicate that microbial amino acid profiles broadly overlap with those of animal cells and traditional meat, but this similarity does not translate into meeting cellular amino acid demand. Consumption data indicate that key amino acids, such as glutamine, cysteine, serine and arginine, would be supplied insufficiently by microbial extracts. This mismatch highlights the need for engineering of microbial biomass and optimization of extraction methods. Extraction methods such as autolysis, enzymatic lysis, or physical disruption strongly influence nutrient release and composition, underscoring the need for standardizing microbial extract preparation. In addition, challenges remain in ensuring consistency, safety, and bioavailability of nutrients, as microbial extracts also contain nucleic acids and potential toxins. This review summarizes current knowledge on microbial hydrolysates for cultivated meat media, including biomass composition, extraction methods, and analytical tools to assess quality and performance. We identify key knowledge gaps, particularly in quantitative amino acid consumption data for relevant cell lines and performance testing in scalable cultures. Addressing these gaps could enable cost-effective media development and accelerate research toward sustainable, commercially viable cultivated meat.
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9. Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.
PMID:日期:2026-10-01Increased prevalence of antimicrobial resistance has led to the urgent need for designing and developing new therapeutic alternatives to conventional antibiotics. Antimicrobial peptides (AMPs) represent a promising class of antimicrobial agents, with broad-spectrum activity and rapid action, and a low potential to promote antibiotic resistance. This review article synthesizes progress in engineering AMPs to optimize effectiveness in terms of enhancing selectivity for treating infections while minimizing cytotoxicity to host cells. Mechanisms by which AMPs exert their effects, such as disrupting membranes, targeting intracellular portions of the cell, inhibiting biofilm formation, and modulating immune response, will be explored in relation to interactions between AMPs and lipid bilayers. The effects of physicochemical properties (e.g., charge and hydrophobicity), peptide length (number of amino acids), and peptide secondary structure on antimicrobial activity and host compatibility are outlined. An overview will be provided of some of the new design approaches for engineering peptides, including peptide modifications, peptide mimetic structures, hybrid peptides, dendritic AMPs, and the use of computational methods and bioengineering techniques. This review highlights current challenges for AMPs, including stability, toxicity, delivery, and drug development for clinical use.
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10. mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.
PMID:日期:2026-10-01Co-occurrence of ammonium (NH -N) and nitrate (NO -N) in wastewaters complicates biological treatment because of opposing oxygen demands and frequent nitrite (NO -N) accumulation. This study evaluates how microscale zero-valent iron (mZVI) influences mixed-nitrogen removal by Klebsiella strain under anaerobic and anoxic conditions. In synthetic mixed-nitrogen tests (NH -N/NO -N = 35/15 mg L), mZVI supplementation accelerated NO -N turnover and increased total nitrogen (TN) removal to ~83% within 48 h; in real wastewater (after C/N adjustment), TN removal rose to ~92%. Electrochemical measurements demonstrated enhanced extracellular electron transfer (EET) in mZVI-amended systems, while fluorescence spectroscopy showed elevated secretion of humic-like substances and flavin-like compounds. Transcriptomic analysis revealed upregulation of pathways and genes associated with nitrate reduction, oxidoreductase activity, cofactor (flavins) biosynthesis, and oxidative phosphorylation, consistent with increased electron flux and energy supply. An integrated mechanism is proposed whereby mZVI stimulates EPS-flavin-mediated EET, coupling extracellular redox enhancement with intracellular metabolic reprogramming to promote efficient denitrification and NH -N assimilation. These results clarify electron-mediated controls on microbial denitrification and support the potential of mZVI as a cost-effective adjunct for treating complex nitrogen-laden wastewaters.