MICROSCOPY RESEARCH AND TECHNIQUE显微研究与技术
MICROSCOPY RESEARCH AND TECHNIQUE(英文缩写 MICROSC RES TECHNIQ),ISSN 1059-910X,eISSN 1097-0029,中文译名:显微研究与技术 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-10-01 至 2026-09-30，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.893 | Q1 |
| 2022 | 2.500 | Q1 |
| 2023 | 2.000 | Q2 |
| 2024 | 2.100 | Q1 |
| 2025 | 2.100 | Q2 |
MICROSCOPY RESEARCH AND TECHNIQUE 最新收录文献
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1. Virtual Staining for Nonlinear Imaging of Cancers.
PMID:日期:2026-10-01Nonlinear imaging such as third-harmonic generation (THG) microscopy can produce label-free images of tissue with histopathological quality, yet the interpretation of label-free images is challenging. Virtual staining can convert label-free images into histology-like images, but its application to nonlinear imaging remains limited even though it has been well established for fluorescence and bright-field imaging. In this review, we summarize the optical principles of several nonlinear imaging modalities, including multiphoton excitation fluorescence, stimulated Raman scattering, and THG microscopy. We then review the recent progression in virtual staining, major network architectures and metrics, and the main challenges for translation into the clinic. In particular, we emphasize the challenges of virtual staining in THG imaging. Unlike molecularly specific contrast mechanisms, THG contrast arises from spatial variations in refractive index and/or third-order nonlinear susceptibility. Consequently, the collected THG signals may reflect multiple structural sources, including water-lipid interfaces and chromatin-associated optical heterogeneity within the nucleus, making it complex and difficult to establish a direct correspondence between THG features and histological labels. By linking imaging to computational methods, this review highlights key directions for advancing THG-oriented virtual staining towards clinical translation.
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2. Deep Learning Assisted-Axial Resolution Improvement for Probe-Based Confocal Laser Endomicroscopy.
PMID:日期:2026-09-17Probe-based confocal laser endomicroscopy (pCLE), as a powerful medical device for detecting digestive tract diseases, can directly conduct real-time and high-resolution histological diagnosis of gastric mucosal epithelium and subcutaneous tissues. It can achieve optical biopsy and has great potential for early diagnosis of gastric cancer. Deep tissue detection and high axial resolution in the depth direction are of great significance for the diagnosis of clinical gastrointestinal diseases. However, improvements in hardware systems will greatly increase the complexity of the system structure, thereby limiting its wide application. Based on the residual channel attention network (RCAN), we developed a deep learning method for axial resolution enhancement. This method, abbreviated as ARE-RCAN, can directly reconstruct images captured by pCLE into ones with higher axial resolution without any additional hardware design. Firstly, high-quality training and testing datasets are generated through numerical simulation, and based on this, the ARE-RCAN is trained and performance tested. The reconstructed results of the testing datasets show that the axial resolution of the reconstructed images of simulated fluorescent beads has doubled, and the lateral resolution has not changed. Finally, a home-built pCLE system was used to collect fluorescent bead and paper fibers data, and the effectiveness of the ARE-RCAN was verified through experiments. The results indicate that the ARE-RCAN method significantly improves the axial resolution of the actual system.
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3. A High-Resolution Transmission Electron Microscopy Image Processing Workflow for Extracting Aromatic Lattice Fringes in High-Rank Coal.
PMID:日期:2026-09-14Quantitative extraction of aromatic lattice fringes from high-resolution transmission electron microscopy (HRTEM) images is essential for the nanoscale characterization of high-rank coal. However, variations in image processing strategies may introduce structural biases into the extracted lattice fringe networks, while the effects of individual processing steps remain insufficiently evaluated. In this study, an image processing workflow was developed for lattice fringe extraction from high-resolution transmission electron microscopy images of high-rank coal. The workflow integrates frequency-domain enhancement, contrast optimization, threshold segmentation, skeletonization, and skeleton refinement. Comparative experiments were performed to evaluate the effects of enhancement strategies, segmentation methods, and skeleton cleaning procedures on the extracted lattice fringe networks. Three topological parameters, including endpoint density, junction density, and average branch length, were introduced to assess skeleton integrity, abnormal connections, and structural continuity. The results demonstrate that selective frequency-domain enhancement is critical for maintaining stable skeleton topology, whereas spatial-domain smoothing increases non-physical connections and fragmentation. Although global Otsu thresholding produces comparable geometric measurements, differences remain in topological characteristics, indicating that geometric parameters alone cannot fully describe lattice fringe networks. Length-based filtering effectively removes artifacts with limited influence on network connectivity, while junction removal disrupts the structural framework of the skeleton. The proposed workflow provides a robust approach for quantitative analysis of lattice fringe networks and offers a methodological basis for reliable microscopic characterization of carbon materials.
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4. Transmission Electron Microscopy-Based Analysis of Silver Nanoparticle-Induced Ultrastructural Damage and Bioaccumulation in Heterakis dispar (Nematoda: Heterakidae) Under In vitro Conditions.
PMID:日期:2026-09-14Helminths cause a wide range of diseases in different components of ecosystems and may induce serious pathological conditions in the host organisms. In recent years, nanoparticles have been increasingly used in ecology, veterinary medicine, and biology. In this study, the effect of silver (Ag) nanoparticles on the waterfowl parasite Heterakis dispar (Schrank, 1790), isolated from domestic geese (Anser anser domesticus L., 1758), was investigated under in vitro conditions, with particular emphasis on nanoparticle accumulation patterns and associated ultrastructural changes, studied at the ultrastructural level. In the experiments, H. dispar nematodes collected from the hosts were exposed in vitro to Ag nanoparticles at concentrations of 10, 50, and 100 μg/mL. Control and experimental groups of nematodes were examined using light and transmission electron microscopes. The sizes of Ag nanoparticles in a free state were 9.03-23.82 nm, and particles detected within the helminth organism were approximately 11-17 nm. It was visually observed that the particles were present in multiple layers of the body wall, in the digestive and reproductive organs of the parasitic worm. Silver nanoparticles enter the helminth through the body wall (from cuticle to the hypodermis, muscular layer, and organs located in the pseudocoelomic or body cavity) and the digestive organs (through the intestinal microvilli). At the ultrastructural level, notable alterations were observed in treated specimens. The results prove that Ag nanoparticles have anthelmintic activity.
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5. Subcellular Localization of Iron in Rhizophora mangle Leaves Revealed by Integrated Perls Reaction, TEM, STEM-HAADF, and EDS Analyses.
PMID:日期:2026-09-07Mangrove ecosystems are frequently exposed to high concentrations of iron (Fe) in sediments, resulting in Fe accumulation in plant tissues. Although Fe is an essential micronutrient involved in several metabolic processes, its excess requires efficient mechanisms of compartmentalization and storage to maintain cellular homeostasis. Histochemical detection using the Perls reaction has usually been applied to identify ferric iron (Fe) in biological tissues; however, the combination of this technique with ultrastructural and elemental analyses remains relatively unexplored in plant cells. In this study, we investigated Fe localization in leaf tissues of Rhizophora mangle L. (Rhizophoraceae), a dominant mangrove species, by combining complementary approaches, including Perls cytochemical reaction, transmission electron microscopy (TEM), scanning transmission electron microscopy coupled with high-angle annular dark-field imaging (STEM-HAADF), and energy-dispersive X-ray spectroscopy (EDS). Perls-positive electron-dense deposits were visualized at the ultrastructural level, and their elemental composition was further characterized by EDS analyses. Fe-containing deposits were detected in the epidermis, mesophyll parenchyma, mucilage cells, and vascular tissues, as well as in multiple cellular compartments, including plastids, mitochondria, vacuoles, cell walls, intercellular spaces, and plasmodesmata, whereas sclerenchyma cells showed no detectable Fe-containing deposits. The combination of Perls reaction with TEM, STEM-HAADF, and EDS provides a complementary approach for high-resolution visualization and elemental characterization of Fe-containing deposits at the subcellular level. This integrated methodology may facilitate the investigation of Fe distribution and compartmentalization in plant tissues under contrasting conditions of Fe availability.
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6. Innovative Method for Evaluating the Optical Properties of Liquid-Liquid Phase Separations Using Laser Scanning Microscopic Spectroscopy.
PMID:日期:2026-09-05Liquid-liquid phase separation (LLPS) plays a central role in intracellular compartmentalization and gene regulation. Although optogenetic optoDroplet systems are widely used to study LLPS in living cells, the mechanisms underlying the transition from dynamic liquid-like condensates to operationally defined gel-like assemblies remain poorly understood because intermediate assembly states are difficult to monitor in situ. Here, we developed a confocal laser microspectroscopy-based approach to characterize the assembly dynamics of the fused sarcomas (FUS)n-mCherry-CRY2 optoDroplet system in living NIH3T3 cells. Quantitative fluorescence analysis enabled the identification and discrimination of three distinct assembly states: monomeric, dimer/oligomer intermediate, and gel-like. Passive micro-rheology, fluorescence recovery after photobleaching (FRAP), and automated morphological analyses further demonstrated progressive reductions in molecular mobility and increasing structural rigidity during condensate maturation. Three-dimensional spatial analysis revealed that mature condensates exhibit a distinct core-shell organization consisting of an operationally defined gel-like core, a dimer/oligomer-rich intermediate layer, and an outer monomer-rich boundary layer with liquid-like properties. Upon cessation of blue-light stimulation, the outer layer rapidly dissolved, whereas the central gel-like core remained intact, indicating the acquisition of physical irreversibility. These findings establish fluorescence microspectroscopy as a quantitative approach for resolving molecular assembly states during condensate maturation and demonstrate that gelation proceeds from the condensate center toward the periphery. This framework provides new insights into pathological liquid-to-gel phase transitions associated with neurodegenerative diseases.
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7. Study on Vessel Morphological Characteristics and Environmental Adaptability of 10 Gentianaceae Species From the Qinghai-Xizang Plateau.
PMID:日期:2026-09-03This study employed scanning electron microscopy (SEM) to conduct a morphological observation of vessel elements from 10 Gentianaceae species collected on the Qinghai-Xizang Plateau. We systematically compared vessel type, end-wall and lateral-wall characteristics, and vessel diameter to explore their adaptive relationship to the extreme plateau environment and the evolutionary trends of vessels. The results indicated that the vessel end walls of all 10 species featured simple perforation plates, while annular, spiral, and scalariform thickenings were observed on the lateral walls of all species, with scalariform vessels constituting the highest proportion in most. The vessel types were primarily annular, spiral, and scalariform; pitted vessels were found only in Gentiana farreri and G. waltonii. The vessel diameters were generally small (mostly < 20 μm). The vessel morphology exhibited significant adaptive characteristics to plateau adversities such as low temperature, drought, and intense radiation. Based on the analysis of vessel type, end wall features, and lateral wall characteristics, the vessels of G. farreri and G. waltonii exhibited the highest proportions of morphologically specialized types (pitted and scalariform vessels) according to the classical Bailey-Frost sequence, whereas those of G. aristata and G. hexaphylla were dominated by the more generalized annular and spiral types. This research provides new anatomical evidence for understanding the structural adaptation mechanisms of Gentianaceae plants in high-altitude environments and offers a theoretical basis for the conservation and utilization of these plant resources.
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8. Interfacial Microstructure and Mechanical Performance of Stainless Steel-Low Alloy Steel Bimetallic Structures Fabricated by Wire Arc Additive Manufacturing.
PMID:日期:2026-09-02Bimetallic structures (BMSs) are being used more frequently in industries like automotive, aerospace, and shipbuilding because they combine different material properties in a single component. Traditional methods like welding and brazing have their limits, but additive manufacturing (AM) offers a more flexible and cost-effective way to create complex multimaterial parts. This study focuses on the microstructure and mechanical properties of BMSs made by depositing stainless steel (SS) on high-strength low-alloy (LA) steel using the Wire Arc Additive Manufacturing (WAAM) process with Surface Tension Transfer (STT). The results show that defect-free BMSs can be successfully fabricated, improving design possibilities. Microstructural analysis revealed a clear Ferrite-Austenite (FA) boundary at the SS-LA interface. Key findings include an 81.81% increase in hardness at the interface relative to the LA steel side, along with an interface tensile strength of 621 ± 7 MPa and elongation of 19.28% ± 1.76%. Electron backscatter diffraction (EBSD) showed fine, randomly oriented grains in the SS region and elongated, directionally aligned grains in the LA region, with no dominant texture, indicating near-isotropic behavior. The presence of both low-angle and high-angle grain boundaries confirmed dynamic recrystallization during deposition. Fracture analysis showed strong mechanical bonding, with failure mainly occurring on the LA side. This demonstrates that WAAM with STT is a viable method for creating reliable, high-performance multimaterial components.
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9. Rapid Identification of Multiple Types of Adulterated Ophiocordyceps sinensis by SEM-EDS Based on Micromorphology and Elemental Analysis.
PMID:日期:2026-09-02As a rare and precious traditional Chinese medicine (TCM), Ophiocordyceps sinensis (O. sinensis) is plagued by various adulterants in the market due to factors such as resource scarcity, which seriously affects medication safety and clinical efficacy. Existing identification methods generally have problems including strong subjectivity, high sample destructiveness, long detection cycle, and complex operation, and it is difficult to identify different types of adulterants using the same method. Innovatively, this study adopted scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDS) technology to establish a rapid, accurate, and widely applicable method for identifying adulterated O. sinensis. Authentic O. sinensis samples from major producing areas such as Xizang, Qinghai, Sichuan, and Gansu, as well as different types of adulterated samples, were collected. Using SEM-EDS technology, combined with micromorphology and elemental composition, the differences between authentic and adulterated samples were systematically revealed through indicators including morphological differences, mass fraction of characteristic elements, and surface area coverage (SAC) of characteristic elements in surface scanning. This study confirmed that SEM-EDS technology could simultaneously obtain the micromorphological and elemental distribution information of samples, realizing rapid and accurate identification of multiple types of adulterated O. sinensis. The proposed method provided new technical support for the quality control and market supervision of O. sinensis, which could be extended to the adulteration inspection of other precious and delicate TCM.
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10. A Robust Method for Microscopic 3D Shape Restoration via Shape-From-Focus.
PMID:日期:2026-08-21Shape-from-focus (SFF) is essential for microscopic three-dimensional morphology analysis due to its non-contact nature and high axial resolution. However, in practical microscopy environments, ambient vibration and multi-source noise often cause unstable sampling and distorted focal volume, significantly limiting the technique's applicability in high-precision measurement. To address this, we propose a novel anti-vibration, high-fidelity microscopic SFF framework. First, a temporal redundancy strategy based on video streaming is employed to generate spatially consistent image sequences prior to reconstruction. Subsequently, an adaptive dual-mode peak localization algorithm is introduced, which intelligently selects depth estimation strategies according to the texture characteristics of the micro-surface, achieving sub-pixel accuracy while maintaining computational efficiency. Furthermore, by transforming one-dimensional focus signals into two-dimensional images and applying an intelligent classifier for filtering, the method successfully separates noise from valid texture features. This framework requires no prior knowledge of vibration frequency and adaptively suppresses random environmental disturbances. Experiments on both synthetic and real microscopic samples show that the proposed method outperforms existing approaches in shape recovery accuracy, preservation of fine texture details, and noise suppression. The technique offers a reliable high-precision 3D measurement solution for materials science, industrial inspection, and biomedical microscopy in vibration-prone environments.