多酚对粘接强度和牙本质酶解的影响

摘要(中文译文)

多酚因其与金属阳离子的相互作用、抗氧化活性及胶原交联能力，已被研究应用于牙科材料和修复技术中，这使其在增强牙体基底和改善材料粘接方面具有前景。然而，在其用于临床操作之前，还需要更多研究来支持。本研究评估了槲皮素和白藜芦醇对粘接强度及牙本质胶原纤维抗酶降解保护作用的影响。将脱矿人牙本质标本分别浸泡于槲皮素或白藜芦醇溶液（100、250和500 µg/mL）、2%氯己定（CHX）或0.9%生理盐水（对照）中1分钟。随后将标本在37°C下于胶原酶中孵育24小时，并测量干质量变化。对于微拉伸粘接强度测试，将槲皮素、白藜芦醇、CHX或蒸馏水（DW）应用于磷酸酸蚀后的牙本质，随后涂布Single Bond 2（3 M/ESPE）粘接剂和Z350XT树脂（3 M/ESPE）。在24小时后及蒸馏水中储存1年后测量粘接强度。数据采用Kruskal-Wallis检验和Dunn检验，或ANOVA后接Tukey检验进行分析（p < 0.05）。与CHX相比，槲皮素处理标本显示重量显著增加（p < 0.05）。槲皮素和白藜芦醇处理组均表现出与CHX相似的抗胶原酶能力（p > 0.05）。1年后，仅DW组显示粘接强度显著下降（p = 0.001）。使用槲皮素或白藜芦醇处理牙本质可有效保持粘接强度并保护胶原免受酶降解，表明它们有望作为改善粘接修复体长期耐久性的 agent。

Abstract

Polyphenols have been investigated in dental materials and restorative techniques due to their interactions with metal cations, antioxidant activity, and collagen crosslinking ability, which make them a promise for reinforcing the dental substrate and improving material adhesion. However, more studies are necessary before to support their use in clinical procedures. This study evaluated the effects of quercetin and resveratrol on bond strength and the protection of dentin collagen fibrils against enzymatic degradation. Demineralized human dentin specimens were immersed for 1 min in solutions of quercetin or resveratrol (100, 250, and 500 µg/mL), 2% chlorhexidine (CHX), or 0.9% saline (control). The specimens were then incubated in collagenase for 24 h at 37°C, and changes in dry mass were measured. For microtensile bond strength testing, quercetin, resveratrol, CHX, or distilled water (DW) were applied to phosphoric acid-conditioned dentin, followed by application of Single Bond 2 (3 M/ESPE) adhesive and Z350XT resin (3 M/ESPE). Adhesive strength was measured after 24 h and after 1 year in distilled water. Data were analyzed using the Kruskal-Wallis and Dunn tests or ANOVA followed by Tukey's test (p < 0.05). Quercetin-treated specimens showed a significant weight increase compared with CHX (p < 0.05). Both quercetin- and resveratrol-treated groups exhibited resistance to collagenase similar to CHX (p > 0.05). After 1 year, only the DW group showed a significant decrease in bond strength (p = 0.001). Dentin treatment with quercetin or resveratrol effectively preserved adhesive strength and protected collagen against enzymatic degradation, suggesting their potential as agents to improve the longevity of adhesive restorations.

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