用于疾病诊断和机制启发发现的细胞外囊泡脂质组学
Extracellular vesicle lipidomics for disease diagnostics and mechanism-informed discovery.
摘要(中文译文)
细胞外囊泡（EVs）是细胞释放的纳米颗粒，其脂质膜包裹分子货物，并有助于囊泡的稳定性、摄取和生物活性。EV的组成编码并传递生物学信息，反映细胞来源、膜重塑、代谢以及疾病相关应激。这使得EV脂质组学在诊断、治疗、机制驱动的发现和新兴生物技术方面具有高度相关性。近期在分离、表征和脂质组学方法方面的进展，使EV脂质谱日益具有可解释性。与此同时，样本生物量低、异质性、共分离颗粒、提取偏倚以及脂质检测、注释和定量置信度不一，仍是重要的设计考量因素。在本综述中，我们讨论近期EV脂质组学研究如何超越非靶向生物标志物发现，转向关于膜适应、细胞来源、细胞间通讯和功能的机制性问题。随后，我们审视其在癌症和神经退行性疾病中的应用，近期工作在这些领域展示了EV脂质组的生物学和生物技术潜力。我们认为，EV脂质组学的下一阶段将需要更强地整合EV表征、质量控制、匹配生物流体比较和功能测定。通过严格的分析设计，EV脂质组学正发展成为机制发现、诊断开发和治疗递送的有力平台。
Abstract
Extracellular vesicles (EVs) are cell-released nanoparticles whose lipid membranes enclose molecular cargo and contribute to vesicle stability, uptake, and biological activity. EV composition encodes and transmits biological information, reflecting cellular origin, membrane remodeling, metabolism, and disease-associated stress. This makes EV lipidomics highly relevant for diagnostics, therapeutics, mechanism-informed discovery, and emerging biotechnology. Recent advances in isolation, characterization, and lipidomics approaches are making EV lipid profiles increasingly interpretable. At the same time, low sample biomass, heterogeneity, co-isolated particles, extraction bias, and variable confidence in lipid detection, annotation, and quantification remain important design considerations. In this review, we discuss how recent EV lipidomics studies are moving beyond untargeted biomarker discovery toward mechanistic questions about membrane adaptation, cellular origin, intercellular communication, and function. We then examine applications in cancer and neurodegeneration, where recent work illustrates the biological and biotechnological potential of EV lipidomes. We argue that the next phase of EV lipidomics will require stronger integration of EV characterization, quality controls, matched biofluid comparisons, and functional assays. With rigorous analytical design, EV lipidomics is evolving into a powerful platform for mechanistic discovery, diagnostic development, and therapeutic delivery.
如何引用
AMA
Danielle Maundy, Fernanda Sousa Monteiro, Adriana Zardini Buzatto. Extracellular vesicle lipidomics for disease diagnostics and mechanism-informed discovery.. Current opinion in biotechnology. 2026; doi:10.1016/j.copbio.2026.103570.
APA
Danielle Maundy, Fernanda Sousa Monteiro, Adriana Zardini Buzatto (2026). Extracellular vesicle lipidomics for disease diagnostics and mechanism-informed discovery.. Current opinion in biotechnology. https://doi.org/10.1016/j.copbio.2026.103570
GB/T 7714
Danielle Maundy, Fernanda Sousa Monteiro, Adriana Zardini Buzatto. Extracellular vesicle lipidomics for disease diagnostics and mechanism-informed discovery.[J]. Current opinion in biotechnology, 2026 doi:10.1016/j.copbio.2026.103570.
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