一种免洗直接SERS平台：通过多重尿液生物标志物检测实现无创肾脏健康监测
A wash-free and direct SERS platform for non-invasive kidney health monitoring via multiplexed urinary biomarker detection.
摘要(中文译文)
尿液生物标志物的常规监测对于慢性肾脏病(CKD)的早期诊断至关重要,然而传统的表面增强拉曼散射(SERS)方法通常需要繁琐的洗涤步骤以去除未结合的报道分子。在此,我们报道了一种免洗涤、多重检测的SERS芯片,用于对白蛋白、尿酸(UA)、肌酐和尿素进行空间分辨分析。该芯片采用微结构碗状PDMS基底组装银纳米颗粒(AgNPs),提供高密度热点以实现灵敏检测。为实现多重尿液分析,我们开发了一种针对分析物特性定制的混合传感策略。对于大分子(白蛋白)和特定代谢物(UA),标记有拉曼报道分子的发夹适配体在结合后发生构象转换,使报道分子远离银表面,从而无需洗涤即可产生定量的“信号关闭”响应。同时,高丰度小分子(尿素和肌酐)通过其特征性的无标记振动指纹进行定量。该“蘸取即读”平台在临床相关范围内展现出优异的线性,并成功分析了模拟患者样本。通过将适配体调控与固有信号检测相结合,本工作为个人肾脏健康管理提供了一种快速、低成本且非侵入性的工具。
Abstract
Routine monitoring of urinary biomarkers is critical for the early diagnosis of chronic kidney disease (CKD), yet conventional surface-enhanced Raman scattering (SERS) methods often require tedious washing steps to remove unbound reporters. Herein, we report a wash-free, multiplexed SERS chip designed for the spatially resolved profiling of albumin, uric acid (UA), creatinine, and urea. The chip features a micro-structured bowl-shaped PDMS substrate assembled with silver nanoparticles (AgNPs), providing high-density hotspots for sensitive detection. To achieve multiplexed urinalysis, we developed a hybrid sensing strategy tailored to the analytes' properties. For macromolecules (albumin) and specific metabolites (UA), hairpin aptamers labeled with Raman reporters undergo a conformational switch upon binding, separating the reporter from the Ag surface and resulting in a quantitative "signal-off" response without washing. Simultaneously, high-abundance small molecules (urea and creatinine) are quantified via their characteristic label-free vibrational fingerprints. This "dip-and-read" platform demonstrates excellent linearity across clinically relevant ranges and successfully analyzes simulated patient samples. By integrating aptamer modulation with intrinsic signal detection, this work provides a rapid, low-cost, and non-invasive tool for personal kidney health management.
如何引用
AMA
Zuyao Wang, Youjiang Zhao, Tingyu Wang, Mengsu Hu, Lei Wu, Shenfei Zong, et al. A wash-free and direct SERS platform for non-invasive kidney health monitoring via multiplexed urinary biomarker detection.. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2027; doi:10.1016/j.saa.2026.128584.
APA
Zuyao Wang, Youjiang Zhao, Tingyu Wang, Mengsu Hu, Lei Wu, Shenfei Zong, et al (2027). A wash-free and direct SERS platform for non-invasive kidney health monitoring via multiplexed urinary biomarker detection.. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. https://doi.org/10.1016/j.saa.2026.128584
GB/T 7714
Zuyao Wang, Youjiang Zhao, Tingyu Wang, Mengsu Hu, Lei Wu, Shenfei Zong, et al. A wash-free and direct SERS platform for non-invasive kidney health monitoring via multiplexed urinary biomarker detection.[J]. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 2027 doi:10.1016/j.saa.2026.128584.
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