基于单倍型的基因组区域验证用于培育新的北欧苹果

摘要(中文译文)

在培育适应北方高纬度地区的苹果品种时，早熟和晚花特性对于应对短生长季及持续的气候变化具有重要价值。根据预期用途的不同，多酚类化合物含量的增加可能被视为有利（果汁和苹果酒用）或不利（鲜食用）。因此，我们在瑞典、挪威和芬兰三个北欧地点，分别对111个、189个和88个苹果基因型的采收期、开花期以及果皮中原花青素B2（PRB2）浓度进行了表型鉴定。利用20K苹果Infinium SNP芯片获得的分型标记数据，我们对先前报道的数量性状位点（QTL）区间及连锁群（LG）3（采收期）、LG 9和12（开花期）以及LG16（PRB2）上的基因组区域进行了基于单倍型的验证。我们评估了这些性状与历史气候适应性之间的相关性，并鉴定了与三个性状增效和减效相关的单倍型。这些结果对北欧育种项目中的标记辅助亲本选择具有重要价值，因为它们支持设计杂交组合以满足当前和未来气候条件的需求，并针对果实品质进行新鲜食型品种或果汁及苹果酒加工型品种的选育。

Abstract

When breeding apple cultivars for northern latitudes, early harvest and late flowering are desirable to manage the short growing season and ongoing climatic changes. Depending on the intended use, increased content of polyphenolic compound might be considered desirable (juice and cider) or undesirable (dessert). Thus, we phenotyped a total of 111, 189, and 88 apple genotypes for harvest date, flowering date, and peel concentration of procyanidin B2 (PRB2), respectively, across three Nordic locations in Sweden, Norway, and Finland. Using phased marker data from the 20 K apple Infinium SNP array we performed haplotype-based validation of previously described quantitative trait loci (QTL) intervals and genomic regions on Linkage Group (LG) 3 (harvest date), LG 9 and 12 (flowering), and LG16 (PRB2). We assessed the correlation between the traits and historical climate adaptation and identified haplotypes associated with increasing and decreasing effects for the three traits. These results are valuable for Marker Assisted Parent Selection in the Nordic breeding programs, as they support designing crosses to meet the demands of current and future climatic conditions, and fruit quality towards breeding of new dessert cultivars, or cultivars for juice or cider production.

如何引用

Hereditas 的其他文献

• LncRNA HOXA11-AS通过海绵吸附miR-148a-3p并调控SMAD2促进特发性肺纤维化进展
• {“_”：“确定FOXK1和SEMA7A是与糖尿病视网膜病变中mA相关程序性细胞死亡相关的关键基因。”，“sup”：[“6”]}