高强度间歇训练改善了健康大鼠在联合使用哌甲酯和乙醇后心脏组织的氧化应激和自噬

摘要(中文译文)

哌甲酯（MPD）与乙醇的合并使用在年轻人群及被诊断为注意缺陷多动障碍（ADHD）的患者中较为普遍。本研究探讨了高强度间歇训练（HIIT）对MPD与乙醇（ET）联合使用所致可能的心脏并发症的影响，尤其关注氧化应激以及线粒体分裂、融合和线粒体自噬。本研究以雄性Wistar大鼠为对象，分别给予生理盐水（CTL）、HIIT、MPD、ET、MPD+ET，或HIIT与MPD、ET或MPD+ET联合干预。采用苏木精-伊红染色和Masson三色染色评估心脏组织损伤和纤维化。采用实时PCR定量mRNA表达，并以比色法测定超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）活性及丙二醛（MDA）水平。与CTL组相比，ET与MPD联合使用可升高MDA水平、降低SOD和GPX活性，并增加DRP1、PINK和Parkin的mRNA表达。在乙醇和MPD处理组中，HIIT与氧化应激及线粒体分裂和线粒体自噬参数的显著降低相关。HIIT减轻了MDA+ET组的组织病理学改变，以及接受ET和MDA与ET联合干预组的纤维化。本研究结果表明，HIIT干预可能与显著减轻MPD和乙醇合并使用对健康雄性大鼠心脏氧化应激、线粒体分裂和线粒体自噬及损伤的不良影响相关。

Abstract

The concurrent consumption of methylphenidate (MPD) and ethanol are prevalent among young individuals and those diagnosed with attention deficit hyperactivity disorder (ADHD). This study investigated the effects of high-intensity interval training (HIIT) on probable cardiac complications caused by the combined use of MPD and ethanol (ET), with a particular focus on oxidative stress and mitochondrial fission, fusion and mitophagy. This study was carried on male Wistar rats that received saline (CTL), HIIT, MPD, ET, MPD+ET, or a combination of HIIT with MPD, ET, or MPD+ET. Cardiac tissue damage and fibrosis were assessed using hematoxylin and eosin and Masson's trichrome staining. The expression of mRNAs was quantified using Real-time PCR, and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) and malondialdehyde (MDA) levels were measured, calorimetrically. The combination of ET and MPD increased the levels of MDA, decreased the activity of SOD and GPX, and increased mRNA expression of DRP1, PINK, and Parkin compared to the CTL group. HIIT was associated with a significant reduction in oxidative stress and mitochondrial fission and mitophagy parameters in ethanol and MPD treated groups. HIIT reduced histopathological changes in MDA+ET group and fibrosis in groups receiving ET and combination of MDA and ET. The findings of this study showed that HIIT intervention could be associated with significant reduction in adverse effects of concurrent consumption of MPD and ethanol on oxidative stress, mitochondrial fission and mitophagy, and injury in heart of healthy male rats.

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