环境水样中PFASs测定的HPLC-UV方法开发：基质效应的深入探讨
Development of an HPLC-UV method for PFASs determination in environmental water samples: Insights into matrix effects.
摘要(中文译文)
全氟和多氟烷基物质（PFASs）是一类存在于环境水体基质中的高毒性化合物。开发一种能够分析和区分PFASs的分析方法，对于支持新兴PFASs降解技术的发展至关重要。大多数分析检测方法依赖于高效液相色谱-质谱联用技术（HPLC-MS），其成本较高且不易普及。其他技术则侧重于带有预浓缩步骤的高效液相色谱-紫外检测法（HPLC-UV），然而不确定性增加，重现性降低。在本研究中，开发了一种无需预处理的HPLC-UV方法，用于定量5种全氟烷基羧酸（PFCAs）：全氟丙酸（PFPrA）、全氟戊酸（PFPeA）、全氟己酸（PFHxA）、全氟庚酸（PFHpA）和全氟辛酸（PFOA）。该定量分析在超纯水、自来水、坑水和饮用水处理厂基质中进行，并分别对单一物质和混合物进行了评估。本工作的主要创新点在于跨真实水基质的方法学验证，依据IUPAC指南对其适用性进行了全面评估，并突出了这些基质如何以化合物依赖的方式影响分析性能。PFPeA、PFHxA、PFHpA和PFOA在任何水基质中的定量限均低于5 mg/L，在混合物中分析时低于10 mg/L。PFPrA由于其短烷基链而最难定量，其校准曲线仅在超纯水中得到验证。进行了ANOVA检验，对于PFHxA、PFHpA和PFOA，未记录到基质间显著的统计学差异。
Abstract
Per- and polyfluoroalkyl substances (PFASs) are a group of highly toxic compounds present in environmental water matrices. The development of an analytical approach capable of analysing and distinguishing PFASs is crucial to support the development of emerging PFASs degradation technologies. Most analytical detection methods rely on High-Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC-MS), which entails considerable costs and is not easily accessible. Other technologies are focused on HPLC with ultraviolet detection (HPLC-UV), with a pre-concentration step; nevertheless, the uncertainty increases, reducing reproducibility. In the present study, an HPLC-UV method without pre-treatment was developed to quantify 5 perfluoroalkylcarboxylic acids (PFCAs): perfluoropropionic acid (PFPrA), perfluoropentanoic acid (PFPeA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA) and perfluorooctanoic acid (PFOA). This quantification was performed in ultrapure, tap, pit and drinking water treatment plant matrices, and they were assessed both individually and in mixture. The main novelty of this work was the methodical validation across real water matrices, which offers a thorough evaluation of its applicability, using IUPAC guidelines, and highlighting how these matrices affect analytical performance in a compound-dependent way. The limit of quantification for PFPeA, PFHxA, PFHpA and PFOA was less than 5 mg L in any water matrix and less than 10 mg L when analysed in mixture. PFPrA was the most difficult to quantify due to its short alkyl chain, and the calibration curve was only validated in ultrapure water. ANOVA tests were conducted and for PFHxA, PFHpA and PFOA significant statistical differences between matrices were not registered.
如何引用
AMA
Catarina Lopes, A Sofia G G Santos, C A Orge, M F R Pereira, O S G P Soares. Development of an HPLC-UV method for PFASs determination in environmental water samples: Insights into matrix effects.. Talanta. 2027; doi:10.1016/j.talanta.2026.130326.
APA
Catarina Lopes, A Sofia G G Santos, C A Orge, M F R Pereira, O S G P Soares (2027). Development of an HPLC-UV method for PFASs determination in environmental water samples: Insights into matrix effects.. Talanta. https://doi.org/10.1016/j.talanta.2026.130326
GB/T 7714
Catarina Lopes, A Sofia G G Santos, C A Orge, M F R Pereira, O S G P Soares. Development of an HPLC-UV method for PFASs determination in environmental water samples: Insights into matrix effects.[J]. Talanta, 2027 doi:10.1016/j.talanta.2026.130326.
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