对大韩民国产肠毒素金黄色葡萄球菌ST1-t321引起的与节日有关的食源性疫情的基因组分析
Genomic insight into a festival-linked foodborne outbreak caused by enterotoxigenic Staphylococcus aureus ST1-t321 in the Republic of Korea.
摘要(中文译文)
本研究调查了韩国某地方节庆期间由金黄色葡萄球菌引起的大规模食源性暴发，并利用全基因组测序（WGS）进行了基因组学和分子流行病学分析。采用临床、食品和环境样本进行微生物学鉴定。分子特征分析包括脉冲场凝胶电泳（PFGE）、多位点序列分型和spa分型。进行WGS以评估克隆相关性、毒力因子和抗菌药物耐药基因。共确定141例疑似病例和18例实验室确诊病例，分离出24株金黄色葡萄球菌（18株来自患者，3株来自食品样本，3株来自环境样本）。纳入基因组分析的17株分离株均携带sea和sec基因，属于ST1-t321，两两差异为8-33个SNP，支持克隆起源。这些分离株在韩国表现出此前未报道的PFGE图谱（STAS16.170）。毒力谱分析鉴定出溶血素基因（hlgA、hlgB、hlgC）、杀白细胞素基因（lukD、lukE）和免疫逃逸基因（sak、scn）。此外，所有分离株均为甲氧西林敏感，且缺乏mecA和mecC。此次大规模食源性暴发表明，单一污染食品来源可在大型集会期间导致广泛发病。基因组分析阐明了暴发菌株的克隆相关性，并凸显了基因组监测对金黄色葡萄球菌暴发早期发现和预防的价值。
Abstract
This study investigated a large-scale foodborne outbreak caused by Staphylococcus aureus during a local festival in the Republic of Korea and performed genomic and molecular epidemiological analyses using whole-genome sequencing (WGS). Microbiological identification was performed using clinical, food, and environmental samples. Molecular characterization included pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, and spa typing. WGS was conducted to evaluate clonal relatedness, virulence factors, and antimicrobial resistance genes. A total of 141 suspected cases and 18 laboratory-confirmed cases were identified, and 24 S. aureus isolates were recovered (18 from patients, 3 from food samples, and 3 from environmental samples). All 17 isolates included in the genomic analysis carried the sea and sec genes and belonged to ST1-t321, with pairwise differences of 8-33 SNPs, supporting a clonal origin. These isolates exhibited a previously unreported PFGE pattern (STAS16.170) in the Republic of Korea. Virulence profiling identified hemolysin genes (hlgA, hlgB, hlgC), leukocidin genes (lukD, lukE), and immune evasion genes (sak, scn). In addition, all isolates were methicillin-susceptible and lacked mecA and mecC. This large-scale foodborne outbreak demonstrated that a single contaminated food source can result in widespread illness during mass gatherings. Genomic analysis clarified the clonal relatedness of the outbreak strains and highlighted the value of genomic surveillance for the early detection and prevention of S. aureus outbreaks.
如何引用
AMA
Yu-Jin Park, Joohyun Han, Wooju Kang, Yeongeun Seo, Mooneui Hong, Soo Young Lee, et al. Genomic insight into a festival-linked foodborne outbreak caused by enterotoxigenic Staphylococcus aureus ST1-t321 in the Republic of Korea.. Osong public health and research perspectives. 2026; doi:10.24171/j.phrp.2026.0022.
APA
Yu-Jin Park, Joohyun Han, Wooju Kang, Yeongeun Seo, Mooneui Hong, Soo Young Lee, et al (2026). Genomic insight into a festival-linked foodborne outbreak caused by enterotoxigenic Staphylococcus aureus ST1-t321 in the Republic of Korea.. Osong public health and research perspectives. https://doi.org/10.24171/j.phrp.2026.0022
GB/T 7714
Yu-Jin Park, Joohyun Han, Wooju Kang, Yeongeun Seo, Mooneui Hong, Soo Young Lee, et al. Genomic insight into a festival-linked foodborne outbreak caused by enterotoxigenic Staphylococcus aureus ST1-t321 in the Republic of Korea.[J]. Osong public health and research perspectives, 2026 doi:10.24171/j.phrp.2026.0022.
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