自适应光学表面等离子体共振全息显微镜的无背景定量相位成像

摘要(中文译文)

近场定量相位成像（QPI）是可视化低维材料、介电混合物和生物细胞中纳米级结构的有力工具。尽管近场QPI具有极高的灵敏度，但光学系统的相位像差可能带来严重限制。为克服这些问题，我们引入了一种自适应光学方法，该方法利用数字全息显微术（DHM）测得的复振幅。通过使用空间光调制器作为光束整形器件，我们的方法能够以Zernike模式量化波前畸变，并利用空间光调制器对其进行预补偿，从而实现原位、准确、快速且灵活的像差校正。为验证该方法，我们在微结构测试样品和活细胞上展示了采用自适应光学表面等离子体共振全息显微术（AO-SPRHM）的近场相位成像。总校正时间低于1秒，使得长达数小时的无背景延时成像成为可能。该方法可轻松迁移至其他相位成像技术，包括透射、反射和全内反射DHM及相关模态。

Abstract

Quantitative phase imaging (QPI) in the near field is a powerful tool for visualizing nanoscale structures in low-dimensional materials, dielectric mixtures and biological cells. Although near-field QPI offers extremely high sensitivity, phase aberrations of the optical system can pose serious limitations. Overcoming these problems, we introduce an adaptive optics approach that takes advantage of the complex amplitude measured by digital holographic microscopy (DHM). By using a spatial light modulator as a beam shaping device, our method allows for in-situ, accurate, fast and flexible aberration correction by quantifying wavefront distortions in terms of Zernike modes, and pre-compensating them with a spatial light modulator. For validation, we demonstrate near-field phase imaging with adaptive-optics surface plasmon resonance holographic microscopy (AO-SPRHM) on microstructured test samples and live cells. With a total correction time below 1 s, background-free time-lapse imaging over many hours becomes feasible. The approach can be easily transferred to other phase imaging techniques, including transmission, reflection and total internal reflection DHM as well as related modalities.

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• 光之人：王占山教授