爱尔兰科克市感染性角膜炎（IK）微生物学特征的12年分析
12-year analysis of the microbiological profile of infectious keratitis (IK) in Cork, Ireland.
摘要(中文译文)
探讨爱尔兰科克大学医院感染性角膜炎（IK）的发生频率及致病病原体。对2010年至2022年间接受角膜刮片/拭子检查的IK病例进行回顾性分析。标本通过实验室信息系统识别。采用培养和/或聚合酶链反应（PCR）检测对棘阿米巴、单纯疱疹病毒（HSV）和水痘-带状疱疹病毒（VZV）进行微生物学特征分析。共分析来自288例患者的319份标本（平均年龄52.0±25.6岁）。总体而言，189份标本（60%）培养阳性。48例（15%）鉴定出多种微生物生长。在198株分离株中，最常鉴定的微生物为凝固酶阴性葡萄球菌（33%）、金黄色葡萄球菌（17%）、铜绿假单胞菌（12%）、肺炎链球菌（8%）和革兰阴性杆菌（8%）。真菌病原体占分离株的4%，混合皮肤菌群占8%。对105份样本进行了革兰染色，22份结果阳性——其中68%与培养结果一致。对48份样本进行了病毒PCR检测，5例（10%）检出HSV-1，2例（4%）检出VZV。对来自319例患者的387份标本进行了棘阿米巴检测，33例（10%）检出棘阿米巴属。这是描述爱尔兰IK微生物学特征的最大规模研究。观察到较高的培养阳性率（60%）及较高的多种微生物分离率（15%），可能受皮肤共生污染菌如凝固酶阴性葡萄球菌的影响。铜绿假单胞菌的比例（12%）低于预期。未发现显著的抗菌药物耐药分离株，支持当前的经验性抗菌治疗策略。
Abstract
To determine the frequency and causative pathogens of infectious keratitis (IK) at Cork University Hospital, Ireland. A retrospective review was performed of IK cases undergoing corneal scraping / swabs between 2010-2022. Specimens were identified from the laboratory information system. Microbiological profiles were analysed using culture and/or polymerase chain reaction (PCR) testing for Acanthamoeba, herpes simplex virus (HSV), and varicella zoster virus (VZV). A total of 319 specimens from 288 patients were analysed (mean age 52.0 ± 25.6 years). Overall, 189 specimens (60%) were culture positive. Polymicrobial growth was identified in 48 cases (15%). Of 198 isolates, the most frequently identified organisms were coagulase-negative staphylococci (33%), Staphylococcus aureus (17%), Pseudomonas aeruginosa (12%), Streptococcus pneumoniae (8%), and Gram-negative bacilli (8%). Fungal pathogens accounted for 4% of isolates, while mixed skin flora was reported in 8%. Gram staining was performed on 105 samples, with 22 positive results-68% were concordant with culture. Viral PCR testing was performed on 48 samples, identifying HSV-1 in 5 cases (10%) and VZV in 2 cases (4%). Acanthamoeba testing was performed on 387 specimens from 319 patients, with Acanthamoeba spp. detected in 33 cases (10%). This is the largest study describing the microbiological profile of IK in Ireland. A high culture-positive rate (60%) with a high rate of isolation of multiple organisms (15%) were observed, likely influenced by skin commensal contaminants such as coagulase-negative Staphylococci. The proportion of Pseudomonas aeruginosa (12%) was lower than expected. No significant antimicrobial resistant isolates were identified, supporting current empiric antimicrobial treatment strategies.
如何引用
AMA
Kealan McElhinney, James Donnelly, Emily Glynn, Anne-Marie Lanigan, Eddie McCullagh, Michael Prentice, et al. 12-year analysis of the microbiological profile of infectious keratitis (IK) in Cork, Ireland.. Irish journal of medical science. 2026; doi:10.1007/s11845-026-04540-7.
APA
Kealan McElhinney, James Donnelly, Emily Glynn, Anne-Marie Lanigan, Eddie McCullagh, Michael Prentice, et al (2026). 12-year analysis of the microbiological profile of infectious keratitis (IK) in Cork, Ireland.. Irish journal of medical science. https://doi.org/10.1007/s11845-026-04540-7
GB/T 7714
Kealan McElhinney, James Donnelly, Emily Glynn, Anne-Marie Lanigan, Eddie McCullagh, Michael Prentice, et al. 12-year analysis of the microbiological profile of infectious keratitis (IK) in Cork, Ireland.[J]. Irish journal of medical science, 2026 doi:10.1007/s11845-026-04540-7.
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