从牛肠道分离的植物乳植杆菌和乳酸片球菌菌株显示出益生菌潜力
Lactiplantibacillus plantarum and Pediococcus acidilactici strains isolated from cattle gut show probiotic potential.
摘要(中文译文)
乳酸菌（LAB），特别是植物乳植杆菌（Lactiplantibacillus plantarum）和乳酸片球菌（Pediococcus acidilactici），是特征明确的益生菌，因其在促进动物肠道健康方面的有益作用而闻名。本研究从牛胃肠道（粪便和唾液）中获得了48株推定乳酸菌。其中，两株菌株（CS-23和BC-14）表现出显著的益生特性，包括耐酸性（67.67-96.33%）、耐胆盐性（49.33-83.60%）、自聚集性（74.24-76.21%）、共聚集性（10.35-23.93%）、细胞表面疏水性（56.10-69.35%）、抗氧化性（DPPH清除率：27.15%和34.66%）以及抗菌活性（抑菌圈：8.3-14.0 mm）。两株菌株对关键抗生素类别（大环内酯类和β-内酰胺类）均敏感，且均未表现出明胶酶活性或溶血活性，表明其使用安全性。基于16S rRNA基因序列分析，两株菌株被鉴定为植物乳植杆菌CS-23和乳酸片球菌BC-14，与动物源本土益生菌株具有密切的系统发育亲缘关系。本土菌株植物乳植杆菌CS-23和乳酸片球菌BC-14展现出良好的益生菌潜力，可作为改善牲畜健康的有效候选菌株。
Abstract
Lactic acid bacteria (LAB), particularly Lactiplantibacillus plantarum and Pediococcus acidilactici, are well-characterized probiotics, known for their beneficial role in promoting gut health in animals. In this study, 48 presumptive LAB were obtained from the cattle gastrointestinal tract (feces and saliva). Among these, two strains (CS-23 and BC-14) exhibited significant probiotic properties, including acid tolerance (67.67-96.33%), bile salt tolerance (49.33-83.60%) auto-aggregation (74.24-76.21%), co-aggregation (10.35-23.93%), cell surface hydrophobicity (56.10-69.35%), antioxidant (DPPH scavenging: 27.15 and 34.66%), and antimicrobial activity (inhibition zone: 8.3-14.0 mm). Both strains were susceptible to key antibiotic classes (macrolides and beta-lactams) and exhibited neither gelatinase nor hemolytic activities, indicating their safety for use. Based on 16 S rRNA gene sequence analysis, the strains were identified as Lactiplantibacillus plantarum CS-23 and Pediococcus acidilactici BC-14, exhibiting close phylogenetic relatedness to indigenous probiotic strains of animal origin. The indigenous strains Lactiplantibacillus plantarum CS-23 and Pediococcus acidilactici BC-14 exhibit promising probiotic potential and could be used as effective candidates for improving livestock health.
如何引用
AMA
Asiya Batool, Humaira Yasmin, Mohammad Abul Farah, Naeem Khan, Muhammad Nadeem Hassan. Lactiplantibacillus plantarum and Pediococcus acidilactici strains isolated from cattle gut show probiotic potential.. BMC microbiology. 2026; doi:10.1186/s12866-026-05266-3.
APA
Asiya Batool, Humaira Yasmin, Mohammad Abul Farah, Naeem Khan, Muhammad Nadeem Hassan (2026). Lactiplantibacillus plantarum and Pediococcus acidilactici strains isolated from cattle gut show probiotic potential.. BMC microbiology. https://doi.org/10.1186/s12866-026-05266-3
GB/T 7714
Asiya Batool, Humaira Yasmin, Mohammad Abul Farah, Naeem Khan, Muhammad Nadeem Hassan. Lactiplantibacillus plantarum and Pediococcus acidilactici strains isolated from cattle gut show probiotic potential.[J]. BMC microbiology, 2026 doi:10.1186/s12866-026-05266-3.
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