{"_":"物种中rDNA和新型串联重复序列的荧光原位杂交定位。","i":["柑橘属"]}

摘要(中文译文)

对林奈1753年所描述物种的细胞遗传学分析因其染色体体积小且形态相似而变得复杂。本研究采用荧光原位杂交（FISH）方法，使用rDNA探针以及基于柑橘基因组中先前未表征的串联重复序列新开发的寡核苷酸探针。四个串联重复序列（CL1、CL14、CL262和CL122）被定位到五个物种的染色体上：林奈1753年描述种、（Christmann，1777）Swingle 1913、（林奈，1753）Osbeck 1765、Rafinesque 1838以及Tanaka 1951。这些重复序列呈现出明显且部分具有物种特异性的分布模式：CL1在所有物种中均被检测到，而CL262、CL122和CL14的定位则更为局限。对其染色体分布与45S和5S rDNA位点关系的比较分析揭示了核糖体DNA保守区域与更为活跃的串联重复序列之间的差异。所鉴定的重复序列可作为有用的细胞遗传学标记，用于该属内的染色体鉴定、核型分析及比较分析。

Abstract

Cytogenetic analysis of Linnaeus, 1753 species is complicated by the small size and morphological similarity of their chromosomes. In this study, we applied the fluorescence hybridization (FISH) method using rDNA probes, as well as newly developed oligonucleotide probes based on previously uncharacterized tandem repeats identified in citrus genomes. Four tandem repeats (CL1, CL14, CL262, and CL122) were mapped to the chromosomes of five species: Linnaeus, 1753, (Christmann, 1777) Swingle, 1913, (Linnaeus, 1753) Osbeck, 1765, Rafinesque, 1838, and Tanaka, 1951. The repeats exhibited distinct and partially species-specific distribution patterns: CL1 was detected in all species, whereas CL262, CL122, and CL14 exhibited more restricted localization. A comparative analysis of their chromosomal distribution in relation to 45S- and 5S-rDNA loci revealed differences between the conserved regions of ribosomal DNA and the more dynamic tandem repeats. The identified repeats represent useful cytogenetic markers for chromosome identification, karyotyping, and comparative analysis within the genus .

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