循环miR-29a和miR-142-5p作为糖尿病肾病生物标志物的潜力：一项横断面研究
Potential of circulating miR-29a and miR-142-5p as biomarkers for diabetic nephropathy: a cross-sectional study.
摘要(中文译文)
糖尿病肾病（DN）是2型糖尿病（T2DM）的主要微血管并发症，也是终末期肾病的主要原因。由于传统生物标志物敏感性有限，早期诊断仍具有挑战性。循环microRNA，包括miR-29a和miR-142-5p，近年来在多种疾病中成为有前景的诊断候选标志物。本研究旨在比较伴有和不伴有DN的T2DM患者血清miR-29a和miR-142-5p水平，并评估其潜在诊断价值。本横断面、单中心观察性研究纳入164例T2DM患者和164名健康对照者。T2DM参与者根据尿白蛋白与肌酐比值（UACR）分为三个亚组。采用定量逆转录PCR测定血清miR-29a和miR-142-5p表达水平。对临床参数进行统计学分析，并进行逻辑回归和受试者工作特征（ROC）曲线分析以评估诊断性能。DN患者血清miR-29a和miR-142-5p水平显著升高，且随疾病严重程度逐渐升高。两种microRNA均被确定为DN的独立危险因素。包含miR-29a、miR-142-5p和胱抑素C的联合模型提高了诊断准确性，曲线下面积（AUC）为0.939。血清miR-29a和miR-142-5p水平升高与DN进展密切相关，支持其作为T2DM患者诊断和风险分层的无创生物标志物的潜力。
Abstract
Diabetic nephropathy (DN), a major microvascular complication of type 2 diabetes mellitus (T2DM), is a leading cause of end-stage renal disease. Early diagnosis remains challenging because conventional biomarkers have limited sensitivity. Circulating microRNAs, including miR-29a and miR-142-5p, have recently emerged as promising diagnostic candidates in several diseases. This study aimed to compare serum levels of miR-29a and miR-142-5p between T2DM patients with and without DN and to evaluate their potential diagnostic utility. This cross-sectional, single-center observational study enrolled 164 patients with T2DM and 164 healthy controls. Participants with T2DM were stratified into three subgroups according to urinary albumin-to-creatinine ratio (UACR). Serum miR-29a and miR-142-5p expression levels were measured using quantitative reverse transcription PCR. Clinical parameters were statistically analyzed, and logistic regression and receiver operating characteristic (ROC) curve analyses were performed to assess diagnostic performance. Serum miR-29a and miR-142-5p levels were significantly elevated in patients with DN and increased progressively with disease severity. Both microRNAs were identified as independent risk factors for DN. A combined model incorporating miR-29a, miR-142-5p, and cystatin C improved diagnostic accuracy, with an area under the curve (AUC) of 0.939. Elevated serum miR-29a and miR-142-5p levels are strongly associated with DN progression, supporting their potential as non-invasive biomarkers for the diagnosis and risk stratification of patients with T2DM.
如何引用
AMA
Qian Liu, Xuanxuan Jiao, Tongdao Xu, Chuanmei He, Fang Yang, Fumeng Yang. Potential of circulating miR-29a and miR-142-5p as biomarkers for diabetic nephropathy: a cross-sectional study.. Frontiers in medicine. 2026; doi:10.3389/fmed.2026.1804671.
APA
Qian Liu, Xuanxuan Jiao, Tongdao Xu, Chuanmei He, Fang Yang, Fumeng Yang (2026). Potential of circulating miR-29a and miR-142-5p as biomarkers for diabetic nephropathy: a cross-sectional study.. Frontiers in medicine. https://doi.org/10.3389/fmed.2026.1804671
GB/T 7714
Qian Liu, Xuanxuan Jiao, Tongdao Xu, Chuanmei He, Fang Yang, Fumeng Yang. Potential of circulating miR-29a and miR-142-5p as biomarkers for diabetic nephropathy: a cross-sectional study.[J]. Frontiers in medicine, 2026 doi:10.3389/fmed.2026.1804671.
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