CD44、CDH6、ITGAV和SERPINE1是癌症的上皮-间充质转移标记物
CD44, CDH6, ITGAV, and SERPINE1 are Epithelial‒Mesenchymal Transition Markers in Gastric Cancer.
摘要(中文译文)
上皮-间质转化（EMT）与胃癌（GC）患者的不良预后相关。传统的EMT标志物被用于量化识别激活的过程。然而，这些分子并非普遍存在。因此，识别更具代表性的EMT标志物对于更好地理解EMT过程、转移及GC进展具有重要意义。本研究采用单因素Cox分析识别与GC预后相关的EMT相关基因，进一步应用Lasso Cox分析筛选候选基因并构建预测GC患者总生存期的特征模型。随后，在GC细胞系中建立EMT组和非EMT组，比较候选基因的表达水平。最后，利用免疫组织化学（IHC）和UALCAN数据库探讨候选基因表达水平与TNM分期之间的关联。从总共200个EMT相关基因中，识别出47个与GC预后相关的EMT相关基因。随后，筛选出12个EMT相关基因作为预测GC患者总生存期的候选基因。该12基因特征模型表现出较强的预测性能。此外，体外分析显示，CD44、CDH6、ITGAV和SERPINE1在EMT组中表达水平上调。同时，CD44、CDH6、ITGAV和SERPINE1的表达水平与TNM分期相关。该12基因特征模型被验证为GC的独立预后因素，凸显了其潜在的预测能力，且这些基因更可能是GC中的EMT标志物。未来需要在更大的人群样本中进一步验证该12基因特征模型的预测性能。CD44、CDH6、ITGAV和SERPINE1在体外模型中被鉴定为EMT标志物，有助于全面理解EMT。然而，这些发现应在后续研究的体内模型中加以验证。CD44、CDH6、ITGAV和SERPINE1与GC预后密切相关，可能是GC中有用的EMT标志物。
Abstract
The Epithelial‒Mesenchymal Transition (EMT) is correlated with poor prognosis in patients with Gastric Cancer (GC). Traditional EMT markers are quantified to identify the activated processes. However, these molecules are not universally present. Thus, identifying more representative EMT markers is important for a better understanding of the EMT process, metastasis, and the progression of GC. Univariate Cox analysis was performed to identify the EMT-related genes associated with GC prognosis. Lasso Cox analysis was further applied to identify candidate genes and generate a signature to predict the overall survival of GC patients. Then, EMT and non-EMT groups were established in GC cell lines to compare candidate gene expression levels. Finally, the associations between the expression levels of the candidates and TNM stage were explored by using immunohistochemistry (IHC) and the UALCAN database. From a total of 200 EMT-related genes, 47 EMT-related genes associated with GC prognosis were identified. Subsequently, 12 EMT-related genes were selected as candidates to predict the overall survival of patients with GC. The 12-gene signature demonstrated strong predictive performance. Furthermore, in vitro analysis revealed that the expression levels of CD44, CDH6, ITGAV, and SERPINE1 were upregulated in the EMT group. In addition, the expression levels of CD44, CDH6, ITGAV, and SERPINE1 were associated with TNM stage. The 12-gene signature was validated to be an independent prognostic factor for GC, highlighting its potential predictive ability, and these genes are more likely to be EMT markers in GC. Future studies are warranted to confirm the predictive performance of the 12-gene signature in larger population samples. CD44, CDH6, ITGAV, and SERPINE1 were identified as EMT markers in an in vitro model, which is conducive to a comprehensive understanding of EMT. However, these findings should be validated in an in vivo model in subsequent studies. CD44, CDH6, ITGAV, and SERPINE1 are strongly related to GC prognosis and may be useful EMT markers in GC.
如何引用
AMA
Xu Han, Lishuang Sun, Wentong Wang, Wei Li, Yunhe Jia, Yangyang Niu, et al. CD44, CDH6, ITGAV, and SERPINE1 are Epithelial‒Mesenchymal Transition Markers in Gastric Cancer.. Current medicinal chemistry. 2026; doi:10.2174/0109298673414770260318052133.
APA
Xu Han, Lishuang Sun, Wentong Wang, Wei Li, Yunhe Jia, Yangyang Niu, et al (2026). CD44, CDH6, ITGAV, and SERPINE1 are Epithelial‒Mesenchymal Transition Markers in Gastric Cancer.. Current medicinal chemistry. https://doi.org/10.2174/0109298673414770260318052133
GB/T 7714
Xu Han, Lishuang Sun, Wentong Wang, Wei Li, Yunhe Jia, Yangyang Niu, et al. CD44, CDH6, ITGAV, and SERPINE1 are Epithelial‒Mesenchymal Transition Markers in Gastric Cancer.[J]. Current medicinal chemistry, 2026 doi:10.2174/0109298673414770260318052133.
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