P38 MAPK通路抑制对炎症期间牙周膜成纤维细胞代谢的影响
Effects of P38 MAPK Pathway Inhibition on the Metabolism of Periodontal Ligament Fibroblasts During Inflammation.
摘要(中文译文)
在牙周炎的发展过程中，破骨细胞的功能被MAPK通路激活。MMPs能够参与交叉激活和自激活级联反应，从而调节破骨细胞分化的基因表达。在MAPK家族中，p38家族对牙周组织慢性炎症的发展有特别显著的影响。p38 MAPK信号传导的激活直接或间接介导炎性细胞因子的表达，从而协同刺激MMP的产生。本实验旨在了解p38 MAPK如何影响用牙龈假单胞菌治疗的炎症性hPDLFs。与对照组相比，经牙龈卟啉单胞菌LPS处理的hPDLFs的MMP-2、-1和-3的分泌显著增加，并且与牙龈卟啉单菌LPS的浓度密切相关。细胞划痕和CCK-8实验表明，MMP-1、-2和-3抑制细胞增殖和运动。为了探索参与p38 MAPK调控的信号通路，hPDLFs单独用牙龈卟啉单胞菌LPS或与p38 MAPK激酶抑制剂联合治疗。结果表明，MMP-1、-2和-3可以作为慢性炎症性疾病牙周炎的唾液生物标志物，并通过p38 MAP激酶途径调节炎症。
Abstract
During the development of periodontitis, osteoclast function is activated by the MAPK pathway. MMPs are able to participate in cross-activation and self-activation cascades, thereby modulating gene expression for osteoclast differentiation. Among the MAPK family, the p38 family has a particularly significant impact on the development of chronic inflammation in periodontal tissue. Activation of p38 MAPK signalling directly or indirectly mediates the expression of inflammatory cytokines, thereby synergistically stimulating MMP production. This experiment aims to understand how p38 MAPK affects inflammatory hPDLFs treated with Pseudomonas gingivalis. Compared with the control group, secretion of MMP-2, -1, and -3 of hPDLFs treated with P. gingivalis-LPS was significantly increased and was closely related to the concentration of P. gingivalis-LPS. Cell scratching and CCK-8 experiments revealed that MMP-1, -2, and -3 inhibited cell proliferation and motility. To explore the signalling pathways involved in p38 MAPK regulation, hPDLFs were treated with P. gingivalis-LPS alone or in combination with a p38 MAPK kinase inhibitor. The results suggest that MMP-1, -2, and -3 can serve as salivary biomarkers for the chronic inflammatory disease periodontitis and regulate inflammation through the p38 MAP kinase pathway.
如何引用
AMA
Cheng Qian, Yifan Gu, Feiyan Gao, Zenan Xu, Jiahui Li, Shuyu Liu, et al. Effects of P38 MAPK Pathway Inhibition on the Metabolism of Periodontal Ligament Fibroblasts During Inflammation.. Journal of cellular and molecular medicine. 2026; doi:10.1111/jcmm.71223.
APA
Cheng Qian, Yifan Gu, Feiyan Gao, Zenan Xu, Jiahui Li, Shuyu Liu, et al (2026). Effects of P38 MAPK Pathway Inhibition on the Metabolism of Periodontal Ligament Fibroblasts During Inflammation.. Journal of cellular and molecular medicine. https://doi.org/10.1111/jcmm.71223
GB/T 7714
Cheng Qian, Yifan Gu, Feiyan Gao, Zenan Xu, Jiahui Li, Shuyu Liu, et al. Effects of P38 MAPK Pathway Inhibition on the Metabolism of Periodontal Ligament Fibroblasts During Inflammation.[J]. Journal of cellular and molecular medicine, 2026 doi:10.1111/jcmm.71223.
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