从桃花中分离的扁桃酰胺可减轻人皮肤细胞中TNF-α驱动的氧化和炎症反应
{"_":"Mandelamide Isolated from Flowers Attenuates TNF-α-Driven Oxidative and Inflammatory Responses in Human Skin Cells.","i":["Prunus persica"]}
摘要(中文译文)
皮肤老化受内在和外在因素共同驱动，包括紫外线（UV）辐射和环境应激因素。肿瘤坏死因子-α（TNF-α）是一种关键的促衰老细胞因子，可促进活性氧（ROS）的产生，导致皮肤细胞中胶原蛋白降解和炎症反应。本研究探讨了某花提取物及其主要成分曼德酰胺（mandelamide）对TNF-α诱导的人真皮成纤维细胞（HDFs）和人表皮角质形成细胞（HEKs）氧化及炎症反应的保护作用。在HDFs中，该提取物及分离化合物显著抑制了TNF-α诱导的ROS生成和基质金属蛋白酶-1（MMP-1）分泌，同时增强了胶原蛋白合成。值得注意的是，曼德酰胺显著降低了MMP-1分泌（从7.53 ± 0.28降至2.97 ± 0.12，P < 0.001），并恢复了胶原蛋白水平（从3.3 ± 0.03升至19.1 ± 0.58，P < 0.001）。在HEKs中，曼德酰胺减弱了TNF-α刺激下炎症介质的产生，并进一步抑制了MMP表达，同时在TNF-α/干扰素-γ（IFN-γ）共刺激下恢复了透明质酸合酶基因的mRNA表达。重要的是，曼德酰胺在炎症条件下表现出选择性活性，而不影响基础细胞状态。综上所述，这些研究结果表明，曼德酰胺是某花的关键生物活性成分，通过调节氧化应激和细胞外基质稳态，对炎症相关皮肤老化发挥保护作用。
Abstract
Skin aging is driven by both intrinsic and extrinsic factors, including ultraviolet (UV) radiation and environmental stressors. Tumor necrosis factor-alpha (TNF-α) is a key pro-aging cytokine that promotes reactive oxygen species (ROS) production, leading to collagen degradation and inflammatory responses in skin cells. In this study, we investigated the protective effects of flower extract and its major constituents (-) against TNF-α-induced oxidative and inflammatory responses in human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs). In HDFs, the extract and isolated compounds significantly suppressed TNF-α-induced ROS generation and matrix metalloproteinase-1 (MMP-1) secretion while enhancing collagen synthesis. Notably, mandelamide () markedly reduced MMP-1 secretion (from 7.53 ± 0.28 to 2.97 ± 0.12, < 0.001) and restored collagen levels (from 3.3 ± 0.03 to 19.1 ± 0.58, < 0.001). In HEKs, mandelamide attenuated the production of inflammatory mediators under TNF-α stimulation and further suppressed MMP expression while restoring the mRNA expression of hyaluronan synthase genes under TNF-α/ interferon-γ (IFN-γ) co-stimulation. Importantly, mandelamide exhibited selective activity under inflammatory conditions without affecting basal cellular states. Collectively, these findings demonstrate that mandelamide is a key bioactive constituent of () flowers and exerts protective effects against inflammation-associated skin aging through the modulation of oxidative stress and extracellular matrix homeostasis.
如何引用
AMA
Yea Jung Choi, Hee Woon Ann, So-Ri Son, Dae Sik Jang, Sullim Lee. {"_":"Mandelamide Isolated from Flowers Attenuates TNF-α-Driven Oxidative and Inflammatory Responses in Human Skin Cells.","i":["Prunus persica"]}. Biomolecules. 2026; doi:10.3390/biom16050672.
APA
Yea Jung Choi, Hee Woon Ann, So-Ri Son, Dae Sik Jang, Sullim Lee (2026). {"_":"Mandelamide Isolated from Flowers Attenuates TNF-α-Driven Oxidative and Inflammatory Responses in Human Skin Cells.","i":["Prunus persica"]}. Biomolecules. https://doi.org/10.3390/biom16050672
GB/T 7714
Yea Jung Choi, Hee Woon Ann, So-Ri Son, Dae Sik Jang, Sullim Lee. {"_":"Mandelamide Isolated from Flowers Attenuates TNF-α-Driven Oxidative and Inflammatory Responses in Human Skin Cells.","i":["Prunus persica"]}[J]. Biomolecules, 2026 doi:10.3390/biom16050672.
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