类风湿关节炎患者外周血中CDYL表达升高
Elevated CDYL expression in the peripheral blood of patients with rheumatoid arthritis.
摘要(中文译文)
染色质结构域Y样蛋白（CDYL）是CDY相关基因家族的一员，通过染色质重塑和干扰正常转录调控，在神经疾病、生殖系统和肿瘤中发挥关键作用。然而，CDYL在类风湿关节炎（RA）中的表达及生物学功能仍不清楚。本研究旨在探讨CDYL在RA发病机制中的作用。研究纳入43例RA患者和36例健康受试者。采用实时定量PCR检测CDYL、白细胞介素-17A（IL-17A）和IL-22的转录水平。采用流式细胞术和Western blot分析CDYL与Th17细胞之间的关系。下载GEO数据库中的GSE100191数据集，对CDYL的潜在功能进行全面的生物信息学分析。采用Western blot检测CDYL与EZH2的相互作用以及EZH2拮抗剂对Th17细胞的影响。CDYL在RA患者外周血单个核细胞中高表达，与DAS28评分（r=0.36，p<0.0177）和血清抗环瓜氨酸肽抗体滴度（r=0.4255，p=0.004）呈正相关。此外，在RA患者和RA数据集中，CDYL转录水平与升高的IL-17A水平（r=0.5140，p<0.001）和IL-22水平（r=0.4540，p=0.002）呈正相关。CDYL在Th17细胞中表达显著上调，而RA患者中Th17细胞比例升高。在机制上，敲低CDYL可显著抑制体外Th17分化，提示CDYL与Th17细胞分化相关并在功能上促进其分化。我们的研究结果表明，CDYL表达与RA患者Th17细胞分化相关并促进其分化，可能参与RA的发病过程。
Abstract
Chromodomain Y-like protein (CDYL), a member of the CDY-related gene family, plays a pivotal role in nervous diseases, reproductive systems and tumors by chromatin remodeling and disrupting normal transcriptional regulation. However, the expression and biological functions of CDYL in rheumatoid arthritis (RA) remain unclear. This study aims to investigated the role of CDYL in the pathogenesis of RA. A cohort of 43 RA patients and 36 healthy subjects were included in the study. The transcript levels of CDYL, interleukin-17 A (IL-17 A) and IL-22 were detected by quantitative real-time PCR. Flow cytometry and Western blot were used to analyze the relationship between CDYL and Th17 cells. The GSE100191 dataset from the GEO database was downloaded to conduct a comprehensive bioinformatics analysis of the potential functions of CDYL. The interaction of CDYL and EZH2 and the effects of the EZH2 antagonist on Th17 cells were measured by Western blot. The CDYL was highly expressed in the PBMCs derived from RA patients, exhibiting positive correlations with DAS28 score (r = 0.36, p < 0.0177) and serum anti-cyclic citrullinated peptide antibody titers (r = 0.4255, p = 0.004). Moreover, the transcript levels of CDYL were positively correlated with elevated IL-17 A levels (r = 0.5140, p < 0.001) and IL-22 (r = 0.4540, p = 0.002) levels in RA patients and the RA dataset. CDYL expression is markedly upregulated in Th17 cells, while the proportion of Th17 cells is increased in RA patients. Mechanistically, CDYL knockdown significantly inhibited Th17 differentiation in vitro, which suggests that CDYL is associated with and functionally contributes to the differentiation of Th17 cells. Our findings indicate that CDYL expression is associated with and promotes the differentiation of Th17 cells in RA patients and may be involved in the process of RA.
如何引用
AMA
Mengxue Xu, Xueli Xia, Jun Yang, Lin Zhao, Juan Xu, Shengjun Wang, et al. Elevated CDYL expression in the peripheral blood of patients with rheumatoid arthritis.. BMC immunology. 2026; doi:10.1186/s12865-026-00847-3.
APA
Mengxue Xu, Xueli Xia, Jun Yang, Lin Zhao, Juan Xu, Shengjun Wang, et al (2026). Elevated CDYL expression in the peripheral blood of patients with rheumatoid arthritis.. BMC immunology. https://doi.org/10.1186/s12865-026-00847-3
GB/T 7714
Mengxue Xu, Xueli Xia, Jun Yang, Lin Zhao, Juan Xu, Shengjun Wang, et al. Elevated CDYL expression in the peripheral blood of patients with rheumatoid arthritis.[J]. BMC immunology, 2026 doi:10.1186/s12865-026-00847-3.
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