两种补骨脂多糖的结构特征和体内外抗骨质疏松潜力
Structural characterization and in vivo and in vitro anti-osteoporosis potential of two Psoralea corylifolia L. polysaccharides.
摘要(中文译文)
补骨脂（Psoralea corylifolia L.，PCL）用于改善骨质疏松已有数千年历史，然而其多糖的抗骨质疏松活性尚未见系统报道。本研究首次证明，PCLP80粗多糖对糖皮质激素诱导的骨质疏松（GIOP）小鼠的股骨骨密度、骨小梁及肌肉功能表现（包括握力和力竭游泳时间）具有显著改善作用（p < 0.05）。随后，从PCLP80组分中纯化得到一种新型酸性多糖（PCLP80-1）和一种中性多糖（PCLP80-2），分子量分别为20.07和53.39 kDa。推测PCLP80-1主要由→3,6)-β-D-Galp-(1→、→6)-β-D-Galp-(1→、β-D-Galp-(1→、→4)-α-D-GalpA-(1→、→3,4)-β-D-Glcp-(1→和α-L-Araf-(1→等片段组成。PCLP80-2主要以→4)-β-D-Manp-(1→、→4,6)-β-D-Manp-(1→、α-D-Manp-(1→、→4)-β-D-Glcp-(1→、α-D-Galp-(1→为特征，并含有少量→3,6)-β-D-Galp-(1→残基。对其高级结构进行了进一步表征。此外，PCLP80-1和PCLP80-2在GIOP小鼠中均表现出明显的抗骨质疏松活性和肌肉功能改善作用。其促进成骨细胞生成的机制主要涉及MG63细胞中的PI3K/AKT/mTOR通路以及MC3T3-E1细胞中的Wnt和Hippo通路。综上所述，本研究有助于从补骨脂中开发高效低毒的药物用于骨质疏松的预防和治疗。
Abstract
Psoralea corylifolia L. (PCL) has been widely used to improve osteoporosis for thousands of years, whereas the anti-osteoporosis activity of its polysaccharides has not been systematically reported. Here, we firstly proved that PCLP80 crude polysaccharides exhibited significant improvement effects (p < 0.05) about femur bone mineral density, bone trabecula, and muscle function performances including grip strengths, and forced swimming times in glucocorticoid-induced osteoporosis (GIOP) mice. Subsequently, a novel acidic (PCLP80-1) and a neutral (PCLP80-2) polysaccharides were purified from PCLP80 fraction, with molecular weights of 20.07 and 53.39 kDa, respectively. PCLP80-1 was proposed to mainly consist of →3,6)-β-D-Galp-(1→, →6)-β-D-Galp-(1→, β-D-Galp-(1→, →4)-α-D-GalpA-(1→, →3,4)-β-D-Glcp-(1→, and α-L-Araf-(1 → moieties. PCLP80-2 was mainly characterized by →4)-β-D-Manp-(1→, →4,6)-β-D-Manp-(1→, α-D-Manp-(1→, →4)-β-D-Glcp-(1→, α-D-Galp-(1 → together with a small amount of →3,6)-β-D-Galp-(1 → residues. Their advanced structures were further characterized. Moreover, PCLP80-1 and PCLP80-2 both showed obvious anti-osteoporosis activity and improvement of muscle function in GIOP mice. The mechanism about increasing osteoblastogenesis mainly involved in PI3K/AKT/mTOR pathway in MG63 cells, Wnt and Hippo pathways in MC3T3-E1 cells. Taken together, this would benefit to develop high efficacy and low toxicity agents from PCL for the prevention and treatment of osteoporosis.
如何引用
AMA
Jing Yang, Ziling Tang, Chanjuan Chen, Pei Lin, Ye He, Xinsheng Yao, et al. Structural characterization and in vivo and in vitro anti-osteoporosis potential of two Psoralea corylifolia L. polysaccharides.. Carbohydrate polymers. 2026; doi:10.1016/j.carbpol.2026.125369.
APA
Jing Yang, Ziling Tang, Chanjuan Chen, Pei Lin, Ye He, Xinsheng Yao, et al (2026). Structural characterization and in vivo and in vitro anti-osteoporosis potential of two Psoralea corylifolia L. polysaccharides.. Carbohydrate polymers. https://doi.org/10.1016/j.carbpol.2026.125369
GB/T 7714
Jing Yang, Ziling Tang, Chanjuan Chen, Pei Lin, Ye He, Xinsheng Yao, et al. Structural characterization and in vivo and in vitro anti-osteoporosis potential of two Psoralea corylifolia L. polysaccharides.[J]. Carbohydrate polymers, 2026 doi:10.1016/j.carbpol.2026.125369.
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