CCCH型锌指基因家族的全基因组鉴定和PbrC3H45在梨木质素生物合成中的功能特征
Genome-wide identification of the CCCH-type zinc finger gene family and functional characterization of PbrC3H45 in lignin biosynthesis of pear.
摘要(中文译文)
梨是世界上最重要的果树作物之一，其果实品质受石细胞含量的强烈影响。CCCH型锌指蛋白在植物生长发育和胁迫响应中发挥核心作用；然而，其在梨石细胞发育过程中的组织特异性表达模式和调控功能仍知之甚少。本研究对梨CCCH型锌指基因家族进行了全基因组分析，鉴定出93个成员（PbrC3H1-PbrC3H93），并基于基因结构和系统发育关系将其分为12个亚家族。基因复制分析表明，该家族的扩张主要由全基因组复制驱动。‘砀山酥梨’多个组织的转录组分析揭示了7个在果实发育过程中高表达的PbrC3H基因。利用206份砂梨种质在石细胞形成关键时期果肉的转录组数据进行共表达网络分析，结果显示PbrC3H45与木质素生物合成相关基因强烈共表达。通过同源和异源遗传转化系统进行的功能验证证实，PbrC3H45正向调控木质素生物合成。这些结果为理解梨CCCH基因家族的进化和组织特异性表达提供了全面的框架。值得注意的是，PbrC3H45被鉴定为石细胞木质化的关键调控因子，为梨果实品质提供了机制性见解，并为遗传改良奠定了基础。
Abstract
Pear is among the world's most important fruit crops, and its fruit quality is strongly influenced by stone cell content. CCCH-type zinc finger proteins play central roles in plant growth, development, and stress responses; however, their tissue-specific expression patterns and regulatory functions during pear stone cell development remain poorly understood. Here, we conducted a genome-wide analysis of the CCCH-type zinc finger gene family in pear and identified 93 members (PbrC3H1-PbrC3H93), which were classified into 12 subfamilies based on gene structure and phylogenetic relationships. Gene duplication analysis indicated that family expansion was mainly driven by whole-genome duplication. Transcriptome profiling across multiple tissues of 'Dangshansuli' revealed seven PbrC3H genes with high expression during fruit development. Co-expression network analysis using transcriptome data from the flesh of 206 sand pear accessions at the critical stage of stone cell formation, showed that PbrC3H45 was strongly co-expressed with lignin biosynthesis-related genes. Functional validation using homologous and heterologous genetic transformation systems confirmed that PbrC3H45 positively regulates lignin biosynthesis. These results provide a comprehensive framework for understanding the evolution and tissue-specific expression of the CCCH gene family in pear. Notably, PbrC3H45 was identified as a key regulator of stone cell lignification, offering mechanistic insights into pear fruit quality and a foundation for genetic improvement.
如何引用
AMA
Chong Pan, Wei Wei, Xianwei Su, Shulin Chen, Kaidi Gu, Pengfei Zheng, et al. Genome-wide identification of the CCCH-type zinc finger gene family and functional characterization of PbrC3H45 in lignin biosynthesis of pear.. Gene. 2026; doi:10.1016/j.gene.2026.150223.
APA
Chong Pan, Wei Wei, Xianwei Su, Shulin Chen, Kaidi Gu, Pengfei Zheng, et al (2026). Genome-wide identification of the CCCH-type zinc finger gene family and functional characterization of PbrC3H45 in lignin biosynthesis of pear.. Gene. https://doi.org/10.1016/j.gene.2026.150223
GB/T 7714
Chong Pan, Wei Wei, Xianwei Su, Shulin Chen, Kaidi Gu, Pengfei Zheng, et al. Genome-wide identification of the CCCH-type zinc finger gene family and functional characterization of PbrC3H45 in lignin biosynthesis of pear.[J]. Gene, 2026 doi:10.1016/j.gene.2026.150223.
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