基于MALDI-MSI的空间代谢组学揭示了三个香榧品种的组织特异性代谢特征
MALDI-MSI-based spatial metabolomics reveals tissue-specific metabolic signatures across three Torreya grandis cultivars.
摘要(中文译文)
香榧（Torreya grandis）是中国南方一种经济上重要的坚果作物，因其丰富的营养成分和生物活性化合物而备受重视，然而其原位代谢物分布至今仍缺乏系统表征。本研究建立了香榧果实的原位代谢图谱，并揭示了三项关键进展。通过MALDI-MSI共鉴定出253种经MS/MS验证的代谢物，包括氨基酸及其衍生物、脂质、生物碱和黄酮类化合物。香榧‘西榧’表现出最高的代谢物丰度，而‘早圆榧’则富含脂质。保守代谢物具有相似的分布位置，但差异性代谢物表现出品种特异性模式。大多数脂质积累在树脂道的中心，生物碱形成外围鞘层，黄酮类化合物富集于假种皮和种仁外层，构成了脂质-生物碱防御模块。分割分析揭示了品种特异性肽段（Arg-Ile-Val-Leu-Val）的定位，提示其可作为潜在的鉴定标记。这些发现为品种鉴定、废弃组织的副产物增值利用以及生物活性化合物的靶向利用提供了空间分辨的基础。
Abstract
Torreya grandis (T. grandis), an economically important nut crop in southern China, is valued for its rich nutrients and bioactive compounds, yet its in situ metabolite distribution remains poorly characterized. This study establishes in situ metabolic atlas of T. grandis fruits and reveals three key advances. A total of 253 MS/MS-validated metabolites were identified by MALDI-MSI, including amino acids and derivatives, lipids, alkaloids, and flavonoids. T. grandis 'Xifei' showed highest metabolite abundance, while 'Zaoyuanfei' was lipid-rich. Conserved metabolites shared similar locations, but distinct metabolites exhibited cultivar-specific patterns. Most lipids accumulate at the center of the resin canals, alkaloids form a peripheral sheath, and flavonoids are enriched in the aril and outer kernel, constituting a lipid-alkaloid defense module. Segmentation analysis revealed cultivar-specific peptide (Arg-Ile-Val-Leu-Val) localization, suggesting a potential identification marker. These findings provide a spatially resolved foundation for cultivar authentication, byproduct valorization of discarded tissues, and targeted utilization of bioactive compounds.
如何引用
AMA
Han Tao, Miaomiao Chen, Yihang Huang, Kexin Liu, Liang Wei, Zuying Zhang, et al. MALDI-MSI-based spatial metabolomics reveals tissue-specific metabolic signatures across three Torreya grandis cultivars.. Food chemistry. 2026; doi:10.1016/j.foodchem.2026.149622.
APA
Han Tao, Miaomiao Chen, Yihang Huang, Kexin Liu, Liang Wei, Zuying Zhang, et al (2026). MALDI-MSI-based spatial metabolomics reveals tissue-specific metabolic signatures across three Torreya grandis cultivars.. Food chemistry. https://doi.org/10.1016/j.foodchem.2026.149622
GB/T 7714
Han Tao, Miaomiao Chen, Yihang Huang, Kexin Liu, Liang Wei, Zuying Zhang, et al. MALDI-MSI-based spatial metabolomics reveals tissue-specific metabolic signatures across three Torreya grandis cultivars.[J]. Food chemistry, 2026 doi:10.1016/j.foodchem.2026.149622.
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