多组学整合绘制了水稻中热、干旱和盐胁迫下CHH甲基化和基因调控网络
Multi-omics integration maps CHH methylation and gene regulatory networks across heat, drought, and salt stress in rice.
摘要(中文译文)
植物表型源于遗传、表观遗传和环境因素之间复杂的相互作用。解析导致表型变异的分子机制需要整合多种基因组、转录组和环境数据集的多组学方法。在此，我们对水稻亚种栽培品种（Nip和93-11）进行了高通量表型分析，涵盖热、干旱和盐胁迫下的关键生长阶段。利用EfficientNet框架，我们生成了用于品种分类和胁迫类型鉴定的图像嵌入。全基因组重亚硫酸盐测序（WGBS）鉴定出在热和干旱胁迫下CHH甲基化水平升高，但在成熟期盐胁迫下降低。此外，我们构建了多组学网络并揭示了热响应子网络。其中，OsCam3在Nip和93-11中均作为热胁迫响应的关键调控因子出现。此外，OsCam3在粳稻和籼稻亚群中经历了不同的选择。总之，我们的研究突出了表观遗传、转录和表型因素在塑造胁迫响应中的相互作用，为未来气候适应性水稻育种提供了宝贵的遗传资源。
Abstract
Plant phenotypes arise from complex interactions among genetic, epigenetic, and environmental factors. Deciphering the molecular mechanisms responsible for phenotypic variation requires comprehensive multi-omics approaches that integrate diverse genomic, transcriptomic, and environmental datasets. Here, we perform high-throughput phenotyping of rice subspecies cultivars (Nip and 93-11), covering key growth stages under heat, drought, and salt stress. Using the EfficientNet framework, we generate image embeddings for cultivar classification and stress-type identification. Whole-genome bisulfite sequencing (WGBS) identifies elevated CHH methylation under heat and drought stresses but reduced levels under salt stress at ripening. Furthermore, we construct a multi-omics network and uncover heat-responsive subnetworks. Within this, OsCam3 emerges as a key regulator of heat stress responses in both Nip and 93-11. Additionally, OsCam3 underwent different selection in the japonica and indica subpopulations. Altogether, our study highlights the interplay among epigenetic, transcriptional, and phenotypic factors in shaping stress responses, providing valuable genetic resources for future climate-resilient rice breeding.
如何引用
AMA
Weijun Guo, Ruihong Qu, Dongwei Li, Shang Xie, Hanlin Liu, Yichao Mao, et al. Multi-omics integration maps CHH methylation and gene regulatory networks across heat, drought, and salt stress in rice.. Cell reports. 2026; doi:10.1016/j.celrep.2026.117352.
APA
Weijun Guo, Ruihong Qu, Dongwei Li, Shang Xie, Hanlin Liu, Yichao Mao, et al (2026). Multi-omics integration maps CHH methylation and gene regulatory networks across heat, drought, and salt stress in rice.. Cell reports. https://doi.org/10.1016/j.celrep.2026.117352
GB/T 7714
Weijun Guo, Ruihong Qu, Dongwei Li, Shang Xie, Hanlin Liu, Yichao Mao, et al. Multi-omics integration maps CHH methylation and gene regulatory networks across heat, drought, and salt stress in rice.[J]. Cell reports, 2026 doi:10.1016/j.celrep.2026.117352.
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