RBPL-1通过其在秀丽隐杆线虫中保留的DWNN结构域促进减数分裂同源配对
RBPL-1 Promotes Meiotic Homolog Pairing Through Its Conserved DWNN Domain in Caenorhabditis elegans.
摘要(中文译文)
减数分裂过程中染色体的准确分离依赖于同源染色体的精确配对。这一过程由一系列特化蛋白驱动，这些蛋白将染色体与细胞骨架马达连接，并协调染色体运动以实现同源染色体的识别与排列。在此，我们鉴定出RBPL-1——秀丽隐杆线虫（Caenorhabditis elegans）中RBBP6的同源蛋白——是一种生殖系表达的调控因子，对同源染色体正确配对及相关的核重组至关重要。RBPL-1的缺失损害了LINC复合体和CHK-2激酶在核周边的簇集形成。此外，我们证明RBPL-1调控ZIM/HIM-8家族蛋白和PLK-2激酶的蛋白丰度，这两者是同源染色体配对的关键介导因子。值得注意的是，RBPL-1在同源染色体配对中的作用不依赖于RING指结构域和Zn指基序，这两个结构域分别被认为介导泛素化和选择性多聚腺苷酸化（APA）/mRNA加工。相反，我们揭示进化上保守但功能尚不明确的DWNN结构域对RBPL-1在同源染色体配对中的功能至关重要。总之，我们的研究结果表明，RBPL-1通过其DWNN结构域，经由介导核重组和控制关键配对因子的丰度，参与减数分裂同源染色体配对。
Abstract
Accurate chromosome segregation during meiosis depends on precise homolog pairing. This process is driven by a series of specialized proteins that link chromosomes to cytoskeletal motors and coordinate chromosome movement for homolog recognition and alignment. Here, we identified RBPL-1, the Caenorhabditis elegans homolog of RBBP6, as a germline-expressed regulator essential for proper homolog pairing and associated nuclear reorganization. Depletion of RBPL-1 impaired the formation of clusters of the LINC complex and CHK-2 kinase within the nuclear periphery. Furthermore, we showed that RBPL-1 regulates the protein abundance of ZIM/HIM-8-family proteins and PLK-2 kinase, two critical mediators of homolog pairing. Notably, RBPL-1's role in homolog pairing is independent of the RING finger domain and Zn knuckle motif, which are proposed to mediate ubiquitination and alternative polyadenylation (APA)/mRNA processing respectively. Instead, we reveal that the evolutionarily conserved yet functionally enigmatic DWNN domain is essential for RBPL-1's function in homolog pairing. In summary, our findings demonstrate that RBPL-1 contributes to meiotic homolog pairing through its DWNN domain, by mediating nuclear reorganization and controlling the abundance of essential pairing factors.
如何引用
AMA
Wencong Nan, Panfeng Li, Guoteng Liu, Lei He, Meiyu Zhang, Hui Gao, et al. RBPL-1 Promotes Meiotic Homolog Pairing Through Its Conserved DWNN Domain in Caenorhabditis elegans.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2026; doi:10.1096/fj.202600516R.
APA
Wencong Nan, Panfeng Li, Guoteng Liu, Lei He, Meiyu Zhang, Hui Gao, et al (2026). RBPL-1 Promotes Meiotic Homolog Pairing Through Its Conserved DWNN Domain in Caenorhabditis elegans.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. https://doi.org/10.1096/fj.202600516R
GB/T 7714
Wencong Nan, Panfeng Li, Guoteng Liu, Lei He, Meiyu Zhang, Hui Gao, et al. RBPL-1 Promotes Meiotic Homolog Pairing Through Its Conserved DWNN Domain in Caenorhabditis elegans.[J]. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2026 doi:10.1096/fj.202600516R.
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