重组木聚糖酶Xyn1m对肉鸡生长性能、肠道形态和细菌群落结构的影响
Effects of recombinant Xylanase Xyn1m on growth performance, Intestinal morphology, and bacterial community structure in broilers.
摘要(中文译文)
本研究评估了来源于琥珀酸丝状杆菌并在毕赤酵母中表达的重组木聚糖酶Xyn1m对肉鸡生长性能、肠道形态及肠道微生物特征的影响。共选取144只1日龄肉鸡，随机分为3个处理组，每组12个重复（每重复4只鸡）：对照组（基础饲粮）、低剂量Xyn1m组（6 800 IU/kg）和高剂量Xyn1m组（13 600 IU/kg）。与对照组相比，高剂量Xyn1m添加显著提高了49日龄体重及29~49日龄生长阶段的平均日增重（P<0.01），而采食量和饲料转化率未受影响。高剂量Xyn1m降低了十二指肠隐窝深度（P<0.05），而回肠形态未见变化。在十二指肠中，Xyn1m添加提高了微生物群落的Shannon指数并降低了Simpson指数（P<0.05），但优势菌群的相对丰度未检测到显著差异。Beta多样性分析表明，高剂量Xyn1m改变了十二指肠微生物群落结构。相比之下，回肠中除不动杆菌属相对丰度呈线性降低外（P<0.05），微生物多样性及群落组成均未见显著差异。基于PICRUSt2的功能预测表明，高剂量组十二指肠微生物群中与营养转运、碳水化合物代谢和能量产生相关的通路得到富集，而回肠中预测的功能变化有限。上述结果表明，饲粮中添加13 600 IU/kg重组木聚糖酶Xyn1m可改善肉鸡生长中期的生长性能，并增强十二指肠微生物多样性和肠道形态，而对远端回肠微生物功能的影响极小。
Abstract
This study evaluated the effects of recombinant xylanase Xyn1m, derived from Fibrobacter succinogenes and expressed in Pichia pastoris, on growth performance, intestinal morphology, and gut microbial characteristics in broilers. A total of 144 one-day-old broilers were randomly allocated to three treatments with 12 replicates per treatment (4 birds per replicate): a control group (basal diet), a low-dose Xyn1m group (6,800 IU/kg), and a high-dose Xyn1m group (13,600 IU/kg). Supplementation with high-dose Xyn1m significantly increased body weight at 49 d and average daily gain during the 29-49 d growth phase compared with the control group (P< 0.01), whereas feed intake and feed conversion ratio were not affected. High-dose Xyn1m reduced duodenal crypt depth (P< 0.05), while ileal morphology remained unchanged. In the duodenum, Xyn1m supplementation increased the Shannon index and reduced the Simpson index of the microbial community (P< 0.05), although no significant differences were detected in the relative abundance of dominant bacterial taxa. Beta-diversity analysis showed that high-dose Xyn1m altered the duodenal microbial community structure. In contrast, no significant differences were observed in microbial diversity or community composition in the ileum, except for a linear reduction in the relative abundance of Acinetobacter (P< 0.05). Functional prediction using PICRUSt2 indicated enrichment of pathways associated with nutrient transport, carbohydrate metabolism, and energy production in the duodenal microbiota of the high-dose group, whereas predicted functional changes in the ileum were limited. These results indicate that dietary supplementation with 13,600 IU/kg recombinant xylanase Xyn1m improved growth performance during the mid-growth stage and enhanced duodenal microbial diversity and intestinal morphology, while exerting minimal effects on distal ileal microbial functions.
如何引用
AMA
Mengjian Liu, Tingting Fu, Shaohua Zhai, Yong Chen. Effects of recombinant Xylanase Xyn1m on growth performance, Intestinal morphology, and bacterial community structure in broilers.. Poultry science. 2026; doi:10.1016/j.psj.2026.106961.
APA
Mengjian Liu, Tingting Fu, Shaohua Zhai, Yong Chen (2026). Effects of recombinant Xylanase Xyn1m on growth performance, Intestinal morphology, and bacterial community structure in broilers.. Poultry science. https://doi.org/10.1016/j.psj.2026.106961
GB/T 7714
Mengjian Liu, Tingting Fu, Shaohua Zhai, Yong Chen. Effects of recombinant Xylanase Xyn1m on growth performance, Intestinal morphology, and bacterial community structure in broilers.[J]. Poultry science, 2026 doi:10.1016/j.psj.2026.106961.
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