癌症预后的分子标志物：使用干细胞相关lncRNA和EMX2OS功能分析的预后模型
Molecular markers for cervical cancer prognosis: a prognostic model using stemness-related lncRNAs and functional analysis of EMX2OS.
摘要(中文译文)
背景：癌症干性相关长链非编码RNA（lncRNAs）在肿瘤发生和进展中发挥关键作用。本研究旨在鉴定宫颈癌（CESC）中与干性相关的lncRNAs，并评估其预后意义、临床相关性及生物学功能。材料与方法：利用癌症基因组图谱的转录组数据构建癌症干性相关基因与CESC特异性lncRNAs的共表达网络，重点关注宫颈鳞状细胞癌和宫颈内膜腺癌。将鉴定出的干性相关lncRNAs通过单因素、LASSO和多因素Cox回归分析建立预后模型。采用实时定量PCR和原位杂交检测正常宫颈组织和癌组织中EMX2OS的表达。应用统计学检验分析EMX2OS表达与临床病理特征之间的关联。通过Cell Counting Kit-8（CCK-8）实验、5-乙炔基-2‘-脱氧尿苷（EdU）实验、平板克隆实验、伤口愈合实验和Transwell实验研究EMX2OS过表达对细胞增殖、迁移和侵袭的影响。结果：建立了一个由五个lncRNAs（FOXD3-AS1、KCNMB2-AS1、EMX2OS、LINC02446、SOCS2-AS1）组成的预后模型，显示出稳健的预后性能。与正常组织相比，EMX2OS在CESC组织中表达显著下调。原位杂交显示EMX2OS表达与肿瘤大小（P = 0.005）、间质浸润深度（P = 0.009）和5年生存状态（P = 0.003）之间存在显著关联。低表达组的5年生存率显著较低（P = 0.006）。EMX2OS过表达显著抑制细胞增殖、迁移和侵袭。结论：基于5个反映CESC干细胞干性的lncRNAs构建的预后模型能够可靠地预测CESC患者的预后。此外，EMX2OS在CESC中是一个重要的肿瘤抑制标志物。
Abstract
BACKGROUND: Cancer stemness-related long non-coding RNAs (lncRNAs) play a crucial role in tumor initiation and progression. This study aimed to identify stemness-related lncRNAs in cervical cancer (CESC) and evaluate the prognostic significance, clinical relevance, and biological functions. MATERIALS & METHODS: Transcriptome data from the Cancer Genome Atlas were used to construct a co-expression network of cancer stemness-related genes and CESC-specific lncRNAs, focusing on cervical squamous cell carcinoma and endocervical adenocarcinoma. Identified stemness-related lncRNAs were used to develop a prognostic model through univariate, LASSO, and multivariate Cox regression analyses. Quantitative real-time PCR and in situ hybridization were performed to measure EMX2OS expression in normal and cancerous cervical tissues. Statistical tests were applied to analyze the association between EMX2OS expression and clinicopathological features. The effects of EMX2OS overexpression on cell proliferation, migration, and invasion were investigated using using the Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2’-deoxyuridine (EdU) assay, plate cloning assay, wound healing assay, and transwell assays. RESULTS: A prognostic model comprising five lncRNAs (FOXD3-AS1, KCNMB2-AS1, EMX2OS, LINC02446, SOCS2-AS1) was developed, demonstrating robust prognostic performance. EMX2OS expression was significantly down-regulated in CESC tissues compared to normal tissues. In situ hybridization showed a marked association between EMX2OS expression and tumor size (P = 0.005), depth of stromal invasion (P = 0.009), and 5-year survival status (P = 0.003). The 5-year survival rate was significantly lower in the low expression group (P = 0.006). Overexpression of EMX2OS significantly suppressed cell proliferation, migration, and invasion. CONCLUSIONS: The prognostic model based on 5 lncRNAs reflecting the stemness of CESC stem cells can reliably predict the prognosis of patients with CESC. Additionally, EMX2OS serves as a significant tumor suppressor marker in CESC.
如何引用
AMA
Xiaochen Liu, Yuandong Liao, Yunyun Liu, Hua Huang, Chunyu Zhang, Shiying Huang, et al. Molecular markers for cervical cancer prognosis: a prognostic model using stemness-related lncRNAs and functional analysis of EMX2OS.. BMC cancer. 2026; doi:10.1186/s12885-026-16022-1.
APA
Xiaochen Liu, Yuandong Liao, Yunyun Liu, Hua Huang, Chunyu Zhang, Shiying Huang, et al (2026). Molecular markers for cervical cancer prognosis: a prognostic model using stemness-related lncRNAs and functional analysis of EMX2OS.. BMC cancer. https://doi.org/10.1186/s12885-026-16022-1
GB/T 7714
Xiaochen Liu, Yuandong Liao, Yunyun Liu, Hua Huang, Chunyu Zhang, Shiying Huang, et al. Molecular markers for cervical cancer prognosis: a prognostic model using stemness-related lncRNAs and functional analysis of EMX2OS.[J]. BMC cancer, 2026 doi:10.1186/s12885-026-16022-1.
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