重组人源化III型胶原蛋白冷凝胶的结构表征及其对HaCaT细胞生物学功能的影响
Structural characterization of recombinant humanized type III collagen cold gel and its effects on the biological functions of HaCaT cells.
摘要(中文译文)
重组人源化胶原蛋白已在生物医学领域得到广泛应用。本研究聚焦于一种新型重组人源化III型胶原蛋白冷凝胶（C3Gel）的表征。为验证C3Gel的结构完整性并探究其细胞相容性及潜在生物学应用，采用涵盖组成、结构、热学性质和形态学的多技术手段进行综合结构分析。此外，通过基于细胞的实验进行了生物学功能评估。结果表明，C3Gel的分子量和结构序列与理论预期一致。在低温条件下，C3Gel自交联形成多孔网络结构，并表现出良好的细胞相容性。它改善了细胞外基质（ECM）的微环境，为HaCaT细胞提供了充分的结构支撑，进而促进了细胞的黏附、增殖和迁移。这些发现表明，C3Gel有望成为一种新型功能性生物医学材料，为该重组胶原蛋白的进一步研究和应用提供了科学依据。
Abstract
Recombinant humanized collagen has been widely used in the biomedical field. This study focuses on the characterization of a novel recombinant humanized type III collagen cold gel (C3Gel). To verify the structural integrity of C3Gel and explore its cytocompatibility and potential biological applications, a multi-technique approach covering composition, structure, thermal properties, and morphology was employed to perform comprehensive structural analysis. In addition, biological functional assessments were conducted using cell-based assays. The results demonstrated that the molecular weight and structural sequence of C3Gel were consistent with theoretical expectations. Under low-temperature conditions, C3Gel self-crosslinked into a porous network structure and exhibited favorable cytocompatibility. It enhanced the microenvironment of the extracellular matrix (ECM) and offered substantial structural support to HaCaT cells, which in turn facilitated their adhesion, proliferation, and migration. These findings suggest that C3Gel holds promise as a novel functional biomedical material, providing a scientific basis for further research and application of this recombinant collagen.
如何引用
AMA
Yizhe Wang, Sibin Kuang, Yuhui Fan, Danfeng Li, Ling Li, Jian Wang, et al. Structural characterization of recombinant humanized type III collagen cold gel and its effects on the biological functions of HaCaT cells.. Frontiers in medicine. 2026; doi:10.3389/fmed.2026.1749460.
APA
Yizhe Wang, Sibin Kuang, Yuhui Fan, Danfeng Li, Ling Li, Jian Wang, et al (2026). Structural characterization of recombinant humanized type III collagen cold gel and its effects on the biological functions of HaCaT cells.. Frontiers in medicine. https://doi.org/10.3389/fmed.2026.1749460
GB/T 7714
Yizhe Wang, Sibin Kuang, Yuhui Fan, Danfeng Li, Ling Li, Jian Wang, et al. Structural characterization of recombinant humanized type III collagen cold gel and its effects on the biological functions of HaCaT cells.[J]. Frontiers in medicine, 2026 doi:10.3389/fmed.2026.1749460.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出