基于人工智能的十二指肠空肠吻合术和袖状胃切除术对Zucker糖尿病脂肪大鼠胰腺形态影响的比较
Artificial intelligence-based comparison of the effects of duodenojejunostomy and sleeve gastrectomy on pancreatic morphology in Zucker diabetic fatty rats.
摘要(中文译文)
本研究旨在比较袖状胃切除术（SG）、十二指肠空肠吻合术（DJOS）及其联合术式（DJOS + SG）对Zucker糖尿病肥胖（ZDF）大鼠血糖调控及胰腺组织形态与功能的影响，并采用人工智能（AI）辅助的组织分析评估形态学改变。45只雄性ZDF大鼠随机分为三个手术组（SG、DJOS、DJOS + SG）。术后1、3和6个月评估口服葡萄糖耐量试验（OGTT）及胰岛素水平。对胰腺组织进行组织学和免疫组织化学分析，检测β细胞质量、PCNA和PDX-1表达。QuPath软件实现了基于AI的腺泡组织、脂肪组织和纤维化组织的定量分析。与SG相比，DJOS和DJOS + SG改善了葡萄糖耐量并提高了空腹胰岛素水平。两种转流术式组均表现出更高的β细胞质量和成簇分布、PCNA和PDX-1表达升高以及更多的腺泡组织。SG与β细胞减少和胰腺脂肪增多相关。吸收不良型（DJOS）或联合减重手术（DJOS + SG）在大鼠模型中显著增强了血糖控制。这些效应伴随β细胞数量增加和成簇分布，以及β细胞增殖和分化的增强。此外，转流手术后腺泡腺体组织增加，而胰腺脂肪沉积减少。
Abstract
This study aims to compare the effects of sleeve gastrectomy (SG), duodenojejunostomy (DJOS), and their combination (DJOS + SG) on glucose regulation and pancreatic histomorphology and function in Zucker diabetic fatty (ZDF) rats, using artificial intelligence (AI)-assisted tissue analysis to assess morphological alterations. Forty-five male ZDF rats were randomized into three surgical groups (SG, DJOS, DJOS + SG). Oral glucose tolerance tests (OGTT) and insulin levels were assessed at 1, 3 and 6 months post-surgery. Pancreatic tissue was analyzed histologically and immunohistochemically for -cell mass, PCNA and PDX-1 expression. QuPath software enabled AI-based quantification of acinar, adipose, and fibrotic tissue. DJOS and DJOS + SG improved glucose tolerance and increased fasting insulin compared to SG. Both bypass groups demonstrated greater -cell mass and clustering, elevated PCNA and PDX-1 expression, and more acinar tissue. SG was associated with reduced -cell presence and increased pancreatic adiposity. Malabsorptive (DJOS) or combination bariatric procedures (DJOS + SG) significantly enhance glycemic control in the rat model. These effects are accompanied by increased -cell numbers and clustering, as well as enhanced -cell proliferation and differentiation. Furthermore, acinar glandular tissue is increased, while pancreatic adiposity is reduced following bypass surgery.
如何引用
AMA
Luisa Schäfer, Ambrus Gabor Mályi, Jodok Fink, Gabriel Seifert, Mira Fink, Stephan Herrmann, et al. Artificial intelligence-based comparison of the effects of duodenojejunostomy and sleeve gastrectomy on pancreatic morphology in Zucker diabetic fatty rats.. Frontiers in surgery. 2026; doi:10.3389/fsurg.2026.1755398.
APA
Luisa Schäfer, Ambrus Gabor Mályi, Jodok Fink, Gabriel Seifert, Mira Fink, Stephan Herrmann, et al (2026). Artificial intelligence-based comparison of the effects of duodenojejunostomy and sleeve gastrectomy on pancreatic morphology in Zucker diabetic fatty rats.. Frontiers in surgery. https://doi.org/10.3389/fsurg.2026.1755398
GB/T 7714
Luisa Schäfer, Ambrus Gabor Mályi, Jodok Fink, Gabriel Seifert, Mira Fink, Stephan Herrmann, et al. Artificial intelligence-based comparison of the effects of duodenojejunostomy and sleeve gastrectomy on pancreatic morphology in Zucker diabetic fatty rats.[J]. Frontiers in surgery, 2026 doi:10.3389/fsurg.2026.1755398.
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