超声辅助提取、纯化、结构表征及粗茎鳞毛蕨根茎多糖抗糖脂代谢紊乱（抗GLMD）活性与机制研究

摘要(中文译文)

鳞毛蕨根茎，俗称“绵马关中”的中药材，在植物学上起源于鳞毛蕨的干燥根茎，具有广泛的药理活性。为了进一步阐述其药用潜力，从鳞毛蕨的干燥根茎中超声辅助提取和纯化了一种均质多糖，命名为DCP-1-2。随后，使用包括HPLC、SERS、甲基化分析和NMR光谱在内的先进分析技术对DCP-1-2进行了全面的结构阐明。值得注意的是，进行了系统的体外评估，以评估DCP-1-2的两个关键生物活性：其抗氧化能力及其对葡萄糖和脂质代谢相关关键酶的抑制作用。为此，作为葡萄糖和脂质代谢途径之间的关键交联，AGEs被用于建立高脂肪细胞模型。然后利用该细胞模型研究DCP-1-2的抗GLMD活性，并破译其潜在的分子机制。总的来说，这些发现为鳞毛蕨多糖在预防和管理GLMD中的潜在应用奠定了坚实的实验基础。

Abstract

Dryopteridis crassirhizomatis Rhizoma, the traditional Chinese medicinal herb commonly known as "Mianma Guanzhong", botanically originating from the dried rhizome of Dryopteris crassirhizoma Nakai, exhibits extensive pharmacological activities. To further elaborate on its medicinal potential, a homogeneous polysaccharide, designated as DCP-1-2, was ultrasonic-assisted extracted and purified from the dried rhizomes of Dryopteris crassirhizoma. Subsequently, comprehensive structural elucidation of DCP-1-2 was conducted using a combination of advanced analytical techniques, including HPLC, SERS, methylation analysis, and NMR spectroscopy. Notably, systematic in vitro assessments were performed to evaluate two key biological activities of DCP-1-2: its antioxidant capacity and its inhibitory effects on key enzymes implicated in glucose and lipid metabolism. To this end, as a critical crosslink between glucose and lipid metabolic pathways, AGEs were employed to establish a high-fat cell model. This cell model was then utilized to investigate the anti-GLMD activity of DCP-1-2 and to decipher the underlying molecular mechanisms. Collectively, these findings lay a solid experimental foundation for the potential application of Dryopteris crassirhizoma-derived polysaccharides in the prevention and management of GLMD.

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