Kisspeptin通过调节PI3K/AKT/GLUT4信号通路改善PCOS患者的局部卵巢胰岛素抵抗
Kisspeptin improves local ovarian insulin resistance in PCOS by modulating the PI3K/AKT/GLUT4 signaling pathway.
摘要(中文译文)
胰岛素抵抗（IR）常见于多囊卵巢综合征（PCOS）患者，影响44%至70%的患者。Kisspeptin是能量平衡和生殖的关键调节因子，它可能通过改善胰岛素抵抗来缓解PCOS相关症状。在这项研究中，使用脱氢表雄酮（DHEA）和高脂肪饮食建立了PCOS-IR小鼠模型。通过免疫荧光染色、定量PCR和Western印迹测量kisspeptin、PI3K、磷酸化PI3K（p-PI3K）、AKT、磷酸化AKT（p-AKT）和葡萄糖转运蛋白4（GLUT4）的表达。流式细胞术用于评估线粒体膜电位（MMP）和活性氧（ROS）水平。在PCOS-IR小鼠的颗粒细胞中，kisspeptin通过激活PI3K/AKT信号通路上调GLUT4的表达。体外实验表明，kisspeptin显著降低了ROS水平，增强了MMP，改善了线粒体功能。Kisspeptin通过PI3K/AKT/GLUT4信号通路改善胰岛素抵抗，并在体外颗粒细胞中发挥作用。通过保护线粒体功能。本研究为PCOS-IR的治疗提供了潜在的生物标志物和治疗靶点。
Abstract
Insulin resistance (IR) is commonly observed in patients with polycystic ovary syndrome (PCOS), affecting 44% to 70% of these individuals. Kisspeptin is a key regulatory factor in energy balance and reproduction, and it may alleviate PCOS-related symptoms by improving insulin resistance. In this study, a PCOS-IR mouse model was established using dehydroepiandrosterone (DHEA) and a high-fat diet. The expression of kisspeptin, PI3K, phosphorylated PI3K (p-PI3K), AKT, phosphorylated AKT (p-AKT), and glucose transporter 4 (GLUT4) was measured by immunofluorescence staining, quantitative PCR, and Western blotting. Flow cytometry was used to evaluate mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) levels. In granulosa cells from PCOS-IR mice, kisspeptin upregulated GLUT4 expression by activating the PI3K/AKT signaling pathway. In vitro experiments showed that kisspeptin significantly reduced ROS levels, enhanced MMP, and improved mitochondrial function. Kisspeptin improves insulin resistance through the PI3K/AKT/GLUT4 signaling pathway and exerts its effects in vitro in granulosa cells. by protecting mitochondrial function. This study provides potential biomarkers and therapeutic targets for the treatment of PCOS-IR.
如何引用
AMA
Na Sun, Pingping Sun, Lilan Sun, Ping Sun, Dandan Qi, Huagang Ma. Kisspeptin improves local ovarian insulin resistance in PCOS by modulating the PI3K/AKT/GLUT4 signaling pathway.. PloS one. 2026; doi:10.1371/journal.pone.0342158.
APA
Na Sun, Pingping Sun, Lilan Sun, Ping Sun, Dandan Qi, Huagang Ma (2026). Kisspeptin improves local ovarian insulin resistance in PCOS by modulating the PI3K/AKT/GLUT4 signaling pathway.. PloS one. https://doi.org/10.1371/journal.pone.0342158
GB/T 7714
Na Sun, Pingping Sun, Lilan Sun, Ping Sun, Dandan Qi, Huagang Ma. Kisspeptin improves local ovarian insulin resistance in PCOS by modulating the PI3K/AKT/GLUT4 signaling pathway.[J]. PloS one, 2026 doi:10.1371/journal.pone.0342158.
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