人参皂苷Rg1调控游离脂肪酸诱导的HepG2细胞肝脂肪变性的作用及机制

摘要(中文译文)

人参皂苷Rg1（G-Rg1）是一种具有生物活性的植物化学物，已被发现对包括非酒精性脂肪性肝病（NAFLD）在内的多种疾病的治疗有益。但目前缺乏关于G-Rg1对NAFLD脂质代谢平衡影响的文献报道。我们研究了G-Rg1对脂质代谢的影响及其机制。我们发现G-Rg1降低了TG、TC和MDA水平，并提高了SOD活性。RT-PCR和western blotting结果显示，在游离脂肪酸诱导后，补充G-Rg1下调了PPAR γ、FABP1、FATP2/5、CD36、SREBP1c和FASN的表达，而上调了PPAR α、CPT1、ACOX1、MTTP和ApoB100的表达。综上所述，我们得出结论：G-Rg1通过调控PPAR α和PPAR γ的表达，抑制脂质合成和脂质摄取，并增强脂质氧化和脂质输出，从而减轻HepG2细胞的肝脂肪变性。

Abstract

Ginsenoside Rg1 (G-Rg1) is a bioactive phytochemical that has been found to be beneficial for the treatment of several diseases including nonalcoholic fatty liver disease (NAFLD). But there is a lack of literature reporting the effect of G-Rg1 on lipid metabolism balance in NAFLD. We investigated the effect and mechanism of G-Rg1 on lipid metabolism . We found that G-Rg1 decreased the levels of TG, TC, and MDA, and increased activity of SOD. Results of RT-PCR and western blotting showed that supplementation with G-Rg1 downregulated the expression of PPAR γ, FABP1, FATP2/5, CD36, SREBP1 c, and FASN, while the expression of PPAR ɑ, CPT1, ACOX1, MTTP, and ApoB100 was upregulated, after induction by a free fatty acid. Taken together, we conclude that G-Rg1 inhibits lipid synthesis and lipid uptake, and enhances lipid oxidation and lipid export to reduce hepatic steatosis of HepG2 cells by regulating PPAR ɑ and PPAR γ expression.

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